Log In Sign up
The largest database of trusted experimental protocols

Biotinylated 8 ohdg antibody

Manufactured by StressMarq Biosciences
Visit Supplier

The Biotinylated 8-OHdG antibody is a laboratory reagent used for the detection and quantification of 8-hydroxy-2'-deoxyguanosine (8-OHdG), a biomarker of oxidative stress. The antibody is conjugated with biotin, which allows for easy detection and signal amplification in various analytical techniques.

Automatically generated - may contain errors

Lab products found in correlation

Lsm 780, by Zeiss (4 mentions) Ab186734, by Abcam (2 mentions) Poly lysine 0.01, by Merck Group (2 mentions) Epr15581, by Abcam (2 mentions) Alexa fluor 555 and alexa 488 donkey anti mouse and rabbit conjugated antibodies, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

8-OHdG) antibody 8-OHdG

2 protocols using biotinylated 8 ohdg antibody

1

Neutrophil Oxidative DNA Damage Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Neutrophils seeded over coverslip chambers (LabTek) pre-coated with poly-lysine 0.01% (Sigma) were fixed in 4% paraformaldehyde overnight and permeabilized in 0.2% Triton for 10 minutes. Oxidized DNA was detected by biotinylated 8-OHdG antibody (StressMarq Biosciences) used at 1 µg/mL and mitochondria (TOM20) was detected using rabbit-anti-human mAb EPR15581 (1:250, ab186734, Abcam), followed by incubation with Alexa Fluor 555 and Alexa 488 donkey anti-mouse and rabbit-conjugated antibodies at 1:250 (Invitrogen) and Hoechst 1:1000. Cells were resuspended in Prolong and visualized with a Zeiss LSM780 laser confocal fluorescence microscope. Images were acquired using the microscope’s associated software.
Lood C., Blanco L.P., Purmalek M.M., Carmona-Rivera C., De Ravin S.S., Smith C.K., Malech H.L., Ledbetter J.A., Elkon K.B, & Kaplan M.J. (2016). Neutrophil extracellular traps enriched in oxidized mitochondrial DNA are interferogenic and contribute to lupus-like disease. Nature medicine, 22(2), 146-153.
+ Open protocol
+ Expand
2

Neutrophil Oxidative DNA Damage Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Neutrophils seeded over coverslip chambers (LabTek) pre-coated with poly-lysine 0.01% (Sigma) were fixed in 4% paraformaldehyde overnight and permeabilized in 0.2% Triton for 10 minutes. Oxidized DNA was detected by biotinylated 8-OHdG antibody (StressMarq Biosciences) used at 1 µg/mL and mitochondria (TOM20) was detected using rabbit-anti-human mAb EPR15581 (1:250, ab186734, Abcam), followed by incubation with Alexa Fluor 555 and Alexa 488 donkey anti-mouse and rabbit-conjugated antibodies at 1:250 (Invitrogen) and Hoechst 1:1000. Cells were resuspended in Prolong and visualized with a Zeiss LSM780 laser confocal fluorescence microscope. Images were acquired using the microscope’s associated software.
Lood C., Blanco L.P., Purmalek M.M., Carmona-Rivera C., De Ravin S.S., Smith C.K., Malech H.L., Ledbetter J.A., Elkon K.B, & Kaplan M.J. (2016). Neutrophil extracellular traps enriched in oxidized mitochondrial DNA are interferogenic and contribute to lupus-like disease. Nature medicine, 22(2), 146-153.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!