Anti fas igm antibody

The anti-Fas (IgM) antibody, anti-phospho-LAT-Tyr226, and rabbit polyclonal anti-human LAT were from Merck-Millipore; anti-PLC-γ1 mAb, anti-Lck, and anti-Erk were from Santa Cruz Biotechnology (Santa Cruz, CA, USA); antibodies binding phospho-Erk, phospho-PLC-γ1-Tyr783, phospho-ZAP70-Tyr319, phospho-MEK-Ser221, phospho-LAT-Tyr191, and anti-MEK were from Cell Signaling Technology; anti-6His-HRP, anti-Grb2, and anti-phospho-LAT-Tyr132 antibodies were from Abcam (Cambridge, MA, USA); and anti-CD69-APC (allophycocyanin) and anti-β-actin monoclonal antibodies were from Biolegend. The protein synthesis inhibitor cicloheximide was purchased from Merck-Millipore. Stimulations were performed with anti-human CD3 (OKT3; eBioscience) or anti-human CD28 (CD28.2; BD Pharmingen) monoclonal antibodies.

The anti-Fas (IgM) antibody was from Merck-Millipore; anti-LAT LAT-01 mAb was from EXBIO (Praha, Czech Republic); anti-LAT 11B.12, anti-PLC- γ, anti-PTP1B, and anti-caspase-3 monoclonal antibodies were from Santa Cruz Biotechnology (Heidelberg, Germany); antibodies binding phospho-Erk, β-actin, phospho-PLC-γ1-Tyr783 and phospho-LAT-Tyr171 were from Cell Signaling Technology; anti-6His-HRP antibody and anti-phospho-LAT-Tyr132 were from Abcam (Cambridge, United Kingdom). The protein synthesis inhibitor cycloheximide was purchased from Merck-Millipore. Stimulations were performed with the anti-human CD3 OKT3 monoclonal antibody (eBioscience).