Donkey antigoat igg h l alexa fluor 594

Manufactured by Thermo Fisher Scientific

Donkey antigoat IgG(H+L) Alexa Fluor 594 is a secondary antibody conjugated with the Alexa Fluor 594 fluorescent dye. It is designed to detect and label goat primary antibodies in immunoassays and other applications requiring fluorescent detection.

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Lab products found in correlation

Alexa fluor 488 donkey anti rabbit igg h l, by Thermo Fisher Scientific (2 mentions) Polyclonal goat anti iba 1, by Abcam (2 mentions) Donkey antirabbit igg h l alexa fluor 594, by Thermo Fisher Scientific (2 mentions) Donkey antimouse igg h l alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Donkey antigoat igg h l alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Polyclonal goat anti lcn2, by R&D Systems (1 mentions) Neuronal nuclei (neun), by Merck Group (1 mentions) Rabbit anti iba1, by Fujifilm (1 mentions) Polyclonal rabbit anti iba1, by Abcam (1 mentions) Mouse monoclonal anti neun, by Merck Group (1 mentions) Alexa fluor 594 donkey anti mouse igg h l, by Thermo Fisher Scientific (1 mentions) Brusatol, by Merck Group (1 mentions)

2 protocols using donkey antigoat igg h l alexa fluor 594

Immunofluorescent Detection of LCN2 and Receptor

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For immunofluorescent double labelling, sections were incubated with 15% normal donkey serum at room temperature for 30 min and then incubated overnight at 4°C with primary antibodies. After washing, sections were incubated with secondary antibodies for 2 h. The primary antibodies were polyclonal goat anti-LCN2 (1:200 dilution, R&D system), polyclonal rabbit anti-SLC22A17 (marker for LCN2 receptor, 1:50 dilution, Abcam), polyclonal mouse anti-GFAP (1:400 dilution, Millipore), monoclonal mouse anti-neuronal nuclei (NeuN; 1:100 dilution, Millipore) and polyclonal goat anti-Iba1 (1:500 dilution, Abcam) or rabbit anti-Iba-1 (1:500 dilution, Wako). The secondary antibodies were donkey antigoat IgG(H+L) Alexa Fluor 594 (1:500 dilution, Invitrogen), donkey antirabbit IgG(H+L) Alexa Fluor 594 (1:500 dilution, Invitrogen), donkey antimouse IgG(H+L) Alexa Fluor 488 (1:500 dilution, Invitrogen), donkey antirabbit IgG(H+L) Alexa Fluor 488 (1:500 dilution, Invitrogen) and donkey antigoat IgG(H+L) Alexa Fluor 488 (1:500 dilution, Invitrogen). The double labelling was analysed using a fluorescence microscope.

Shishido H., Toyota Y., Hua Y., Keep R.F, & Xi G. (2016). Role of lipocalin 2 in intraventricular haemoglobin-induced brain injury. Stroke and Vascular Neurology, 1(2), 37-43.

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Microglial Activation and Regulation Mechanisms

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The primary antibodies used in this study included polyclonal goat anti‐Iba1 (Abcam, 1:400), polyclonal rabbit anti‐Iba1 (Abcam, 1:400), polyclonal rabbit anti‐heme oxygenase‐1 (Enzo, 1:500), monoclonal mouse anti‐TLR9 (Abcam, 1:400), polyclonal rabbit anti‐TLR9 (GeneTex, 1:400), polyclonal goat anti‐GFAP (Abcam, 1:400), monoclonal rabbit anti‐Darpp‐32 (Cell Signaling, 1:100), polyclonal rabbit anti YM‐1 (Abcam, 1:200), monoclonal mouse anti‐NeuN (MERCK, 1:400), monoclonal mouse anti‐Arg1 (Santa Cruz, 1:200), polyclonal rabbit anti‐CD16 (Abcam, 1:200), polyclonal rabbit anti‐iNOS (Abcam, 1:200), polyclonal rabbit anti‐CD36 (GeenTex, 1:2000), monoclonal rabbit anti‐CD204 (Abcam, 1:2000), And polyclonal rabbit anti‐Nrf2 (Proteintech, 1:2000).
The secondary antibodies used in this article for immunofluorescence staining included donkey anti‐rabbit IgG (H + L) Alexa Fluor 488, donkey anti‐rabbit IgG (H + L) Alexa Fluor 594, donkey anti‐mouse IgG (H + L) Alexa Fluor 488, donkey anti‐mouse IgG (H + L) Alexa Fluor 594, donkey anti‐goat IgG (H + L) Alexa Fluor 488, and donkey anti‐goat IgG (H + L) Alexa Fluor 594, all obtained from Invitrogen.
The drugs used in this study included CpG ODN1826 and CpG ODN2138 (InvivoGen), clodronate liposomes, control liposomes (Liposoma Technology), and brusatol (Sigma‐Aldrich).

Wei J., Dai S., Pu C., Luo P., Yang Y., Jiang X., Li X., Lin W, & Fei Z. (2022). Protective role of TLR9‐induced macrophage/microglia phagocytosis after experimental intracerebral hemorrhage in mice. CNS Neuroscience & Therapeutics, 28(11), 1800-1813.

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