Protease max solution

Manufactured by Promega

Sourced in Sweden

Protease MAX solution is a concentrated proteolytic enzyme formulation designed for use in protein extraction and purification workflows. It is a versatile tool that can efficiently degrade a wide range of protein substrates.

Automatically generated - may contain errors

Lab products found in correlation

Saccharomyces cerevisiae enolase digest, by Waters Corporation (3 mentions) Sequencing grade trypsin, by Promega (3 mentions) Glu1 fibrinopeptide b glu1 fib, by Waters Corporation (2 mentions) Iodoacetamide, by Bio-Rad (2 mentions) Resuspension buffer, by Promega (2 mentions) Pbs solution, by Thermo Fisher Scientific (2 mentions) Dithiolthreitol dtt, by Bio-Rad (2 mentions) Protease inhibitors cocktail, by Roche (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Iodoacetamide, by Merck Group (1 mentions) Sodium dodecyl sulfate (sds), by Merck Group (1 mentions) Dithiothreitol (dtt), by Merck Group (1 mentions) Lys c, by Promega (1 mentions) Trypsin and lys c, by Promega (1 mentions) Trypsin, by Promega (1 mentions)

4 protocols using protease max solution

Protein Extraction and Digestion

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lysis buffer (7M urea, 2M thiourea, 4% CHAPS, 70mM DTT and protease inhibitor cocktail), dithiothreitol (DTT), SDS, iodoacetamide, ammonium bicarbonate were obtained from Sigma (St Louis, MO). Sequencing-grade trypsin and protease MAX solution were purchased from Promega (Madison, WI). Protease inhibitors cocktails were purchased from Roche (Mannheim, Germany). Coomassie (Bradford) protein assay kit was obtained from Pierce (Rockford, IL). [Glu¹]-fibrinopeptide B ([Glu¹]-Fib) and Saccharomyces cerevisiae enolase digest were obtained from Waters (Milford, MA). All solutions were prepared using MS-grade water from J.T Baker (Center Valley, PA).

Huang L., Wickramasekara S.I., Akinyeke T., Stewart B.S., Jiang Y., Raber J, & Maier C.S. (2016). Ion mobility-enhanced MSE-based Label-free Analysis Reveals Effects of Low-dose Radiation Post Contextual Fear Conditioning Training on the Mouse Hippocampal Proteome. Journal of proteomics, 140, 24-36.

+ Open protocol

+ Expand

Efficient Protein Digestion Protocol

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Proteins were digested by trypsin and LysC (Promega). trypsin hydrolyzes the ester bonds on the carboxyl side of Arg, while trypsin and LysC hydrolyze the ester bonds on the carboxyl side of Lys. The combination of trypsin and LysC enhances the efficiency of digestion. Therefore, trypsin/LysC digestion was carried out as described previously [27 (link)]. To the supernatant obtained from the above steps, 90 μL of 6 M urea was added and shaken for about 10 min at RT using a tube mixer. Then, 360 μL of 0.1 M Tris-HCl (pH 8.5) was added, and ultrasonic treatment and standing on ice were repeated twice for 30 s using an ultrasonic washer (Branson 2510, Danbury, CT, USA) to resuspend the protein precipitate. Next, 10 μL of 0.5 mg mL⁻¹ LysC solution and 25 μL of 1% Protease Max solution (Promega, Nacka, Sweden) were added and mixed by tapping, and the samples were incubated at 25 °C for 3 h. Finally, 10 μL of 0.5 mg mL⁻¹ trypsin solution was added and mixed by tapping, and the samples were incubated at 37 °C for 16 h.

Toyoshima M., Tokumaru Y., Matsuda F, & Shimizu H. (2020). Assessment of Protein Content and Phosphorylation Level in Synechocystis sp. PCC 6803 under Various Growth Conditions Using Quantitative Phosphoproteomic Analysis. Molecules, 25(16), 3582.

+ Open protocol

+ Expand

Mass Spectrometry Protein Identification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dithiolthreitol (DTT) and iodoacetamide were purchased from Bio-Rad (Hercules, CA). MS-grade water, acetonitrile and formic acid (99%) were obtained from EMD Chemicals (Gibbstown, NJ). Sequencing-grade trypsin, resuspension buffer and protease MAX solution were purchased from Promega (Madison, WI). [Glu^{1 (link)}]-fibrinopeptide B ([Glu^{1 (link)}]-Fib) and Saccharomyces cerevisiae enolase digest were obtained from Waters (Milford, MA). PBS solution was purchased from Fisher (Fair Lawn, NJ).

Motorykin I., Traber M.G., Tanguay R.L, & Maier C.S. (2014). Proteome-Driven Elucidation of Adaptive Responses to Combined Vitamin E and C Deficiency in Zebrafish. Journal of Proteome Research, 13(3), 1647-1656.

+ Open protocol

+ Expand

Peptide Identification Mass Spectrometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dithiolthreitol (DTT) and iodoacetamide were
purchased from Bio-Rad (Hercules, CA). MS-grade water, acetonitrile,
and formic acid (99%) were obtained from EMD Chemicals (Gibbstown,
NJ). Sequencing-grade trypsin, resuspension buffer, and protease MAX
solution were purchased from Promega (Madison, WI). [Glu¹]-fibrinopeptide B ([Glu¹]-Fib) and Saccharomyces
cerevisiae enolase digest were obtained from Waters (Milford,
MA). PBS solution was purchased from Fisher (Fair Lawn, NJ).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!