Gp91 ds tat

Manufactured by Eurogentec

Sourced in United States

Gp91 ds-tat is a laboratory equipment product manufactured by Eurogentec. It is a protein that serves as a core function in various research applications. The detailed description of its intended use and capabilities is not available in an unbiased and factual manner while maintaining conciseness.

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Lab products found in correlation

Angptl4, by Thermo Fisher Scientific (1 mentions) Z vad fmk, by Merck Group (1 mentions) Ferrostatin 1, by Merck Group (1 mentions) Liproxstatin 1, by Merck Group (1 mentions) Tazs89a, by Addgene (1 mentions) Vinculin, by Santa Cruz Biotechnology (1 mentions) Yap taz, by Cell Signaling Technology (1 mentions) V5 tag, by Cell Signaling Technology (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Tempol, by Merck Group (1 mentions) Verapamil, by Merck Group (1 mentions) Acetylcholine, by Merck Group (1 mentions) Phenylephrine hydrochloride, by Merck Group (1 mentions)

3 protocols using gp91 ds tat

Molecular Mechanisms of Ferroptosis Regulation

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Erastin was obtained from the Duke University Small Molecule Synthesis Facility. The following antibodies, their catalog numbers, sources and diltuionswere indicated below: YAP/TAZ (#8418, Cell Signaling Technology, 1:1000), βa-tubulin (#86298, Cell Signaling Technology, 1:2000), vinculin (sc-73614, Santa Cruz, 1:2000), V5 tag (#13202, Cell Signaling Technology, 1:2000), H3 (#4499, Cell Signaling Technology, 1:2000), GAPDH (sc-25778, Santa Cruz, 1:2000), ANGPTL4 (#40–9800, ThermoFisher Scientific, 1:1000), NOX2 (sc-130543, Santa Cruz, 1:1000), anti-rabbit IgG, horseradish peroxidase (HRP)-linked antibody (#7074, Cell Signaling Technology, 1:2000–1:4000) and anti-mouse IgG, HRP-linked Antibody (#7072, Cell Signaling Technology, 1:2000–1:4000). Plasmids were obtained from Addgene (TAZS89A #52084; ANGPTL4-V5 #102446). The NOX2 inhibitor, gp91 ds-tat, was purchased from Eurogentec (cat #: AS-63818) and recombinant human ANGPTL4 protein was purchased from Novus Biologicals (4487-AN). VAS2870 (Calbiochem-492000), GKT136901 (Calbiochem-534032), Z-VAD-FMK (SML0583), ferrostatin-1 (SML0583) and liproxstatin-1 (SML1414) were purchased from Sigma.

Yang W.H., Huang Z., Wu J., Ding C.K., Murphy S.K, & Chi J.T. (2019). A TAZ-ANGPTL4-NOX2 axis regulates ferroptotic cell death and chemoresistance in epithelial ovarian cancer. Molecular cancer research : MCR, 18(1), 79-90.

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Canstatin-mediated Angiogenesis Inhibition

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The reagent sources were as follows: recombinant mouse canstatin (produced by Escherichia coli as described previously [15 (link)]), verapamil and tempol (Sigma-Aldrich, St. Louis, MO, USA), gp91 ds-tat (Eurogentec, Seraing, Belgium) and H₂O₂ (Kanto Chemical, Tokyo, Japan). PBS (NaCl 137 mM, KCl 2.7 mM, Na₂HPO₄ 10 mM, KH₂PO₄ 1.8 mM, pH 7.4) was used as a vehicle for recombinant canstatin.

Sugiyama A., Shimizu Y., Okada M., Otani K, & Yamawaki H. (2021). Preventive Effect of Canstatin against Ventricular Arrhythmia Induced by Ischemia/Reperfusion Injury: A Pilot Study. International Journal of Molecular Sciences, 22(3), 1004.

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Vasodilation and Platelet Activation

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Phenylephrine hydrochloride and acetylcholine was obtained from Sigma-Aldrich (St. Louis, MO). The gp91 ds-tat was obtained from Eurogentec (USA). Thromboxane analogue (U46619) was purchased from Calbiochem (USA).

Kassan M., Ait-Aissa K., Ali M., Trebak M, & Matrougui K. (2015). Augmented EGF receptor tyrosine kinase activity impairs vascular function by NADPH oxidase-dependent mechanism in type 2 diabetic mouse. Biochimica et biophysica acta, 1853(10 Pt A), 2404-2410.

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