Rvd1 and rvd2

Cytokine and chemokine levels were measured from the cell-free BALF of the first PBS wash using murine-specific ELISAs. We examined the levels of TNF-α, IL-6, CXCL1, AREG, GM-CSF, MPO, MCP-1, and MIF using commercially available kits (Duoset ELISA development kits, R&D Systems) as well as RvD1 and RvD2 (Cayman Chemical) according to the manufacturer’s instructions.

Recombinant human or mouse IL-17A was purchased from R&D Systems. The D series resolvins, RvD1 and RvD2, and MK2206 (Akt inhibitor) were from Cayman Chemical. SB202190 (p38 MAP kinase inhibitor), SB216763 (GSK-3β inhibitor), SN50 (NF-κB inhibitor), SP600125 (JNK inhibitor) were obtained from EMD-Millipore. LY294002 (PI3K inhibitor) and LY30351 (inactive analogue of LY294002) were purchased from Sigma-Aldrich. Rabbit polyclonal antibody to Del-1 was from ProteinTech. Rabbit monoclonal antibody (mAb) to β-actin (13E5), rabbit IgG antibodies to total Akt (phosphorylation state independent), phospho-Akt (Ser473), total GSK-3β and phospho-GSK-3β (Ser9), as well as rabbit anti-phospho-C/EBPβ (Thr-188) and anti-phospho-threonine IgG antibodies were purchased from Cell Signaling Technology. Rabbit IgG antibodies to GPR32 and to ALX/FPR2 were from Abcam and Abnova, respectively. FITC- or PE-conjugated goat anti-rabbit IgG and rabbit IgG antibody to C/EBPβ were purchased from Santa Cruz Biotechnology. All reagents (including those mentioned in the description of assays below) were used at doses based on our previously published work or on experiments in this study (see Supplementary Figs 1 and 4).