Antibodies were commercially purchased including mouse monoclonal antibody anti-PGK1 (Santa Cruz Biotechnology, Cat. sc-130335; RRID: AB_627677), mouse monoclonal antibody anti-phosphate-tyrosine (anti-pY; Santa Cruz Biotechnology, Cat. sc-7020; RRID: AB_628123), rabbit polyclonal antibodies anti-acetylated lysine (anti-Ac; Cell Signaling Technology, Cat. 9441), rabbit monoclonal antibody anti-β-actin (ABclonal, Cat. AC026), mouse anti-GFP-Tag mAb (ABclonal, Cat. AE012), mouse anti-His-Tag mAb (ABclonal, Cat. AE003), mouse control IgG (ABclonal, Cat. AC011), and alkaline phosphatase-labeled goat anti-rabbit or horse anti-mouse IgG secondary antibodies (ZSGB-BIO, Cat. ZB-2308; ZB-2310). ATG8 and Caspase3 were detected with rabbit polyclonal antibodies against H. armigera ATG8 and Caspase3 that were prepared in our laboratory.
Mouse control igg
Manufactured by ABclonal
Sourced in United States
Mouse control IgG is a laboratory reagent used as a control in various immunoassays and experiments involving mouse-derived samples or antibodies. It serves as a reference to ensure the specificity and accuracy of the tested antibodies or assays.
Automatically generated - may contain errors
Lab products found in correlation
Anti pgk1, by Santa Cruz Biotechnology (1 mentions)
Mouse anti gfp tag mab, by ABclonal (1 mentions)
Mouse anti his tag mab, by ABclonal (1 mentions)
Veriblot for ip detection reagent hrp, by Abcam (1 mentions)
Nedd8, by Abcam (1 mentions)
Protein a g bead, by Santa Cruz Biotechnology (1 mentions)
Gapdh mouse mab, by Cell Signaling Technology (1 mentions)
Sp600125, by Cell Signaling Technology (1 mentions)
C jun rabbit mab, by Cell Signaling Technology (1 mentions)
Icrt14, by MedChemExpress (1 mentions)
β actin rabbit mab, by Cell Signaling Technology (1 mentions)
Alexa fluor 488 conjugated goat anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions)
P jnk thr183 tyr185 rabbit mab, by Cell Signaling Technology (1 mentions)
Lamina c mouse mab, by Santa Cruz Biotechnology (1 mentions)
β tubulin rabbit pab, by ABclonal (1 mentions)
Rabbit control igg, by ABclonal (1 mentions)
Goat anti rabbit igg, by Cell Signaling Technology (1 mentions)
Normal rabbit igg, by Cell Signaling Technology (1 mentions)
Classic magnetic protein a g ip co ip kit, by Ipsen (1 mentions)
4 protocols using mouse control igg
1
Antibody Detection for Protein Analysis
2
Immunoprecipitation of Coro1a Protein
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Cells were solubilized in Co‐IP lysis buffer containing the components listed above and 1% SDS (v/v) by rigorous scraping of the cells while on ice. For IP under denaturing conditions, the cell lysate was incubated at 100°C for 5 min. The lysate was then diluted 10 times with lysis buffer without SDS and IP with the indicated antibody mouse control IgG (Abclonal) and the anti‐Coro1a antibody (Santa Cruz) and the addition of protein A/G beads (Santa Cruz) or M2‐Flag beads (Sigma) alone overnight at 4°C. The immunocomplexes were then washed at least three times in lysis buffer, resolved by SDS‐PAGE, and analysed by WB with the indicated antibodies to Coro1a, NEDD8 (Abcam) and VeriBlot for IP Detection Reagent (HRP) (Abcam) or Flag (CST) and regular secondary antibodies.
3
Investigating JNK/c-Jun Signaling Pathway
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The following chemical reagents were used in this study: iCRT14 (MedChemExpress, cat# HY16665), sp600125 (Cell Signaling Technology, cat#8177). The following antibodies were used in this study: phospho(p)-c-Jun (Ser73) rabbit monoclonal antibody (Cell Signaling Technology, cat# 3270), c-Jun rabbit mAb (Cell Signaling Technology, cat# 9165), p-JNK (Thr183/Tyr185) rabbit mAb (Cell Signaling Technology, cat# 9251), JNK rabbit polyclonal antibody (pAb) (Cell Signaling Technology, cat# 9252). p-β-catenin (Ser552) rabbit mAb (Cell Signaling Technology, cat# 9566). β-catenin (Ser552) rabbit mAb (Abcam, cat# ab32572), mouse control IgG (ABclonal, cat# AC011), rabbit control IgG (ABclonal, cat# AC005), laminA/C mouse mAb (Santa Cruz Biotechnology, cat# sc-376248), β-Tubulin rabbit pAb (Abclonal, cat# AC015), GAPDH mouse mAb (Cell Signaling Technology, cat# 2118), β-actin rabbit mAb (Cell Signaling Technology, cat# 4970), Alexa Fluor 488®-conjugated goat anti-rabbit IgG (H + L) (Invitrogen, cat# A-11008), HRP- (horseradish peroxidase-) conjugated goat anti-mouse IgG (Cell Signaling Technology, cat# 7076), and goat anti-rabbit IgG (Cell Signaling Technology, cat# 7074). Goat anti-BoHV-1 serum was purchased from VMDR Inc (cat# 20PAB-IBR).
4
Analyzing Protein Interactions and Phosphorylation
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Physical interactions between proteins and the phosphorylation status of target proteins were analyzed by the Classic Magnetic Protein A/G IP/Co-IP Kit (YJ201, Epizyme, China) in conjunction with WB analysis. The inputs used for WB in co-IP assays were 10% whole-cell lysates. For the negative control, bait proteins were captured with Normal Rabbit IgG (2729, Cell Signalling Technology, USA) or Mouse Control IgG (AC011, ABclonal, China). Information on the primary antibodies used to capture bait proteins for the co-IP assays is available in Supplementary file 1—table 1 .
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