Mouse anti bax antibody clone 6a7

BAX activation was determined by immunoprecipitation using active conformation-specific antibodies. Briefly, cells were lysed in CHAPS lysis buffer (10 mmol/L HEPES, pH 7.4; 150 mmol/L NaCl; 1% CHAPS). 500 μg protein were incubated overnight at 4°C with 8 μg mouse anti-BAX antibody (clone 6A7; Sigma-Aldrich) and 10 μL panmouse IgG Dynabeads (Dako, Hamburg, Germany), washed with CHAPS lysis buffer and analyzed by Western blotting using rabbit anti-BAX NT antibody.

BAK and BAX activation was determined by immunoprecipitation of active conformation by specific antibodies. Briefly, cells were lysed in CHAPS lysis buffer (10 mmol/l HEPES, pH 7.4; 150 mmol/l NaCl; 1% CHAPS). 500 μg protein was incubated overnight at 4°C with 8 μg mouse anti-BAX antibody (clone 6A7; Sigma) or 0.5 μg mouse anti-BAK antibody (AB-1; Calbiochem, Darmstadt, Germany) and 10 μl pan mouse IgG Dynabeads (Dako, Hamburg, Germany), washed with CHAPS lysis buffer and analyzed by Western blotting using rabbit anti-BAX NT antibody (Millipore, Darmstadt, Germany) or rabbit anti-BAK antibody (BD Biosciences). Loss of MMP was assessed by JC-1 staining according to the manufacturer's instructions (ThermoFisher scientific).

BAK and BAX activation was determined by immunoprecipitation as previously described [21 (link)]. Briefly, cells were lysed in CHAPS lysis buffer (10 mmol/l HEPES, pH 7.4; 150 mmol/l NaCl; 1% CHAPS). 500 μg protein was incubated over night at 4°C with 0.5 μg mouse anti-BAK antibody (AB-1; Calbiochem) or 8 μg mouse anti-BAX antibody (clone 6A7; Sigma) and 10 μl pan-mouse IgG Dynabeads, washed with CHAPS lysis buffer, and analyzed by Western blotting using rabbit anti-BAK antibody or rabbit anti-BAX NT antibody.