Skno 1

Kasumi-1, SKNO-1 (JCRB Cell Bank, Osaka, Japan), HMC-1.1 (Merck Millipore, Darmstadt, Germany), HMC-1.2 and pt18 cells were cultured at 37 °C in RPMI1640 medium supplemented with 10% FCS, penicillin, streptomycin, glutamine (Pen/Strep/Gln), and reducing agents (0.5 mM monothioglycerol or 50 μM 2-mercaptoethanol). For expansion of SKNO-1, 10 ng/mL granulocyte macrophage colony-stimulating factor (GM-CSF, Peprotech, Rocky Hill, NJ) was used. GIST-T1 cells (Cosmo Bio, Tokyo, Japan) were cultured at 37 °C in DMEM supplemented with 10% FCS and Pen/Strep/Gln. All human cell lines were authenticated by Short Tandem Repeat analysis at JCRB Cell Bank (Osaka, Japan) and tested for Mycoplasma contamination with a MycoAlert Mycoplasma Detection Kit (Lonza, Basel, Switzerland).

The human AML cell lines (THP1, NOMO1, Kasumi-1 and SKNO-1) were obtained from State Key Laboratory of Experimental Hematology. THP1, NOMO1 and Kasumi-1 cells were cultured at 1-2 × 10⁶ cells/mL in RPMI-1640 (C11875500BT, GIBCO, USA) containing 10% fetal bovine serum (FBS, C04001, Vivacell, China), 1% penicillin/streptomycin (SV30010, HyClone, USA). In addition to the above components, cell culture medium for SKNO-1 was supplemented with 10 ng/ml GM-CSF (300-03, PeproTech, USA). Cells were maintained at 37°C in a humidified incubator (ThermoFisher, USA).