No content assay kit

Manufactured by Beyotime

Sourced in China

The NO content assay kit is a laboratory product designed to quantify the levels of nitric oxide (NO) in various samples. It provides a reliable and accurate method for measuring NO concentrations.

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Caspase activity assay kit, by Beyotime (1 mentions)

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NO kits (3 citations)

2 protocols using no content assay kit

Quantifying Chondrocyte Inflammatory Markers

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To detect the levels of NO and PGE2, chondrocytes were exposed to IL-1β (10 ng/ml) with or without different concentrations of Dio. Then, the culture supernatant was collected for subsequent measurements. The NO level was determined by estimating the nitrite concentration based on Griess reaction with a NO content assay kit (Beyotime, Nanjing, China). The PGE2 level was detected with an ELISA kit (R&D Systems, MN, USA). All procedures followed the instructions of the manufacturer and all assays were performed in triplicate.

Ding Q., Zhang R., Sheng G., Wang T., Jing S., Ma T., Wang S., Zhao H., Wu H, & Li W. (2023). Dioscin alleviates the progression of osteoarthritis: an in vitro and in vivo study. Journal of Inflammation (London, England), 20, 14.

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Assessing Oxidative Stress and Apoptosis

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The cells and muscle tissue were collected for measuring the production of NO and assaying the activities of caspase-3 and caspase-9 by using NO Content Assay Kit (No. S0021, Beyotime Biotechnology, Shanghai, China) and Caspase Activity Assay Kits (No. C1115, and No. C1157, Beyotime Biotechnology, Shanghai, China) according to the manufacturer’s instructions, respectively. The samples of muscle tissue from all groups were placed in 4% paraformaldehyde. All of the tissue sections were made and imaged by the company Pinuofei Biotechnology (Wuhan, China).

Liang B, & Su J. (2019). Inducible Nitric Oxide Synthase (iNOS) Mediates Vascular Endothelial Cell Apoptosis in Grass Carp Reovirus (GCRV)-Induced Hemorrhage. International Journal of Molecular Sciences, 20(24), 6335.

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