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Medium molecular weight chitosan powder

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Medium molecular weight chitosan powder is a natural biopolymer derived from the exoskeletons of crustaceans. It is a versatile material used in various applications, including pharmaceutical, biomedical, and industrial sectors.

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Lab products found in correlation

Ldh kit, by Roche (1 mentions) Non essential amino acid (neaa), by Thermo Fisher Scientific (1 mentions) Glacial acetic acid, by Merck Group (1 mentions) Cucl2 h2o, by Merck Group (1 mentions) Kh2po4, by Merck Group (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions) Sodium hydroxide (naoh), by Merck Group (1 mentions) Gluconic acid δ lactone, by Merck Group (1 mentions) Sodium tetraborate decahydrate, by Merck Group (1 mentions) Silver nitrate and sodiumthiosulfate, by Merck Group (1 mentions) Safranin o staining kit, by ScienCell (1 mentions) Bs 10423r, by Bioss Antibodies (1 mentions) Bs 0709r, by Bioss Antibodies (1 mentions) Alizarin red s staining kit, by Abcam (1 mentions) Masson s trichrome, by Bio-Optica (1 mentions) Penicillin streptomycin, by Lonza (1 mentions) L glutamine, by Lonza (1 mentions) Alcian blue, by ScienCell (1 mentions) Glycerol, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Medium molecular weight chitosan Chitosan powder Chitosan

5 protocols using medium molecular weight chitosan powder

1

Antibacterial Polymer Nanocomposite Synthesis

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WS2 powder, having an average nanoparticle size of 80 to 100 nm, was purchased from Lower Friction Company (Mississauga, ON, Canada). P(3HB-co-12 mol% HHx) powder (350,000 Da) was obtained from KANEKA Corporation, Osaka, Japan. Medium molecular weight chitosan powder was purchased from Sigma-Aldrich, St. Louis, MO, USA. Glacial acetic acid (99.8%) was obtained from Sigma-Aldrich, Petaling Jaya, Malaysia. Bacterial cultures of E. coli K1 and MRSA were grown and incubated for up to 16 h prior to antibacterial analysis. LDH kit was purchased from Roche Diagnostics, Indianapolis, IN, USA. Non-essential amino acid was purchased from Life Technologies, Carlsbad, CA, USA. In this experiment, analytical-grade reagents were used.
Mukheem A., Shahabuddin S., Akbar N., Ahmad I., Sudesh K, & Sridewi N. (2022). Development of Biocompatible Polyhydroxyalkanoate/Chitosan-Tungsten Disulphide Nanocomposite for Antibacterial and Biological Applications. Polymers, 14(11), 2224.
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2

Chitosan Crosslinking for Biomaterials

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Medium molecular weight chitosan powder (~250,000 g/mol) with a DD of 75–85% was obtained from Sigma-Aldrich (St. Louis, MO, USA). A glutaraldehyde (GA) solution (25%) was purchased from SHOW (Tokyo, Japan) and used as a crosslinking agent. HCl, NaOH, CuCl∙2H2O, and KH2PO4 were purchased from Sigma-Aldrich (St. Louis, MO, USA). All chemicals were used as received without further purification.
Kim T., Shin J, & An B. (2023). Adsorption Characteristics for Cu(II) and Phosphate in Chitosan Beads under Single and Mixed Conditions. Polymers, 15(2), 421.
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3

Chitosan-Gluconic Acid Hydrogel Synthesis

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Medium-molecular-weight chitosan powder was
purchased from Sigma-Aldrich. Gluconic acid δ-lactone (GDL)
and sodium tetraborate decahydrate (borax) were supplied from Sigma-Aldrich
(Italy). All solutions were prepared using deionized Milli-Q water
(resistivity 18.2 MΩ.cm).
Vaughan E., Santillo C., Imbrogno A., Gentile G., Quinn A.J., Kaciulis S., Lavorgna M, & Iacopino D. (2023). Direct Laser Writing of Chitosan–Borax Composites: Toward Sustainable Electrochemical Sensors. ACS Sustainable Chemistry & Engineering, 11(37), 13574-13583.
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4

Chitosan-Helix aspersa Scaffold Fabrication

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Medium-molecular-weight chitosan powder (Sigma-Aldrich), Helix aspersa mucus extract (Medical Grade, Xi’an
SR Bio-Engineering Co., Ltd.), and Helix aspersa slime
extract (Pharmaceutical Grade) (Xi’an Nate Biological Technology
Co., Ltd.) were used for scaffold fabrication. Eagle’s MEM
(Capricorn Scientific), penicillin/streptomycin, and l-glutamine
(Lonza) were used in cell culture studies. The WST-1 assay (BioVision
Inc.), Enzyline PAL Optimise ALP kit (Biomerieux, France), Human Osteocalcin
and Human Cartilage Oligomeric Matrix Protein (COMP) Sandwich-ELISA
Kits (Elabscience), and proteoglycan assay (Amsbio, AMS Biotechnology)
were used for in vitro bioactivity studies. Hematoxylin
& eosin (Mayer’s), Masson’s Trichrome (Bio-Optica,
04-010802), Alcian Blue (ScienCell 8378), Alizarin Red S Staining
kit (Abcam ab146374), Safranin O Staining kit (ScienCell 8348), type
I collagen (bs-10423R, Bioss), and type II collagen (bs-0709R, Bioss)
were used for histology staining protocols. Silver nitrate and sodium
thiosulfate (Sigma-Aldrich) were used for the biomineralization study.
Tamburaci S., Perpelek M., Aydemir S., Baykara B., Havitcioglu H, & Tihminlioglu F. (2023). Fabrication of Helix aspersa Extract Loaded Gradient Scaffold with an Integrated Architecture for Osteochondral Tissue Regeneration: Morphology, Structure, and In Vitro Bioactivity. ACS Applied Bio Materials, 6(4), 1504-1514.
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5

Chitosan Coatings with Essential Oils

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Chitosan coatings were prepared according to the method of Pabast et al. [22 (link)], with some modifications. The chitosan solution (2%) was made by dissolving medium molecular weight chitosan powder (Sigma-Aldrich, St. Louis, MO, USA) in 1% acetic acid (>99.8%, Centrohem, Stara Pazova, Serbia) by stirring with a magnetic stirrer (SCILOGEX SCI280-Pro, Rocky Hill, CT, USA) for 2 h at 95 °C. After filtering the resulting solutions, 0.25 mL/g of glycerol (98+%, Fisher Scientific, Loughborough, UK) was added to the chitosan solution. Stirring was continued for 30 min at room temperature. Thereafter, a surfactant, Tween 60 (Fisher Scientific, Loughborough, UK), at a concentration of 0.1% was added and stirring was continued at 60 °C for 30 min. After cooling to room temperature, Satureja montana L. essential oil (SMEO) or nanoparticles in concentrations of 0.25%, 0.5% and 1% was added to the chitosan coating.
Đorđević N., Karabegović I., Cvetković D., Šojić B., Savić D, & Danilović B. (2022). Assessment of Chitosan Coating Enriched with Free and Nanoencapsulated Satureja montana L. Essential Oil as a Novel Tool for Beef Preservation. Foods, 11(18), 2733.
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