Q500 ultrasonic processor

Manufactured by Qsonica

Sourced in United States

The Q500 ultrasonic processor is a laboratory instrument designed for sample preparation, cell disruption, and homogenization. It utilizes high-frequency sound waves to disrupt and disperse materials in liquid samples.

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Lab products found in correlation

Nexion 350d, by PerkinElmer (1 mentions) Ripa buffer, by Merck Group (1 mentions) Nanodrop 2000c spectrophotometer, by Thermo Fisher Scientific (1 mentions) Protease and phosphatase inhibitor, by Thermo Fisher Scientific (1 mentions)

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Ultrasonic processor (9 citations)

4 protocols using q500 ultrasonic processor

Electro-precipitation and Emulsion Analysis

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The electro-precipitation method was
performed using a WaveDriver 200 and WaveVortex (Pine Instruments,
Durham, NC) with the chronoamperometry technique (induction at 0 mV
for 3 s, step potential at −1.5 V for 300 s, and relaxation
at 0 mV for 1 s). The emulsion was prepared using a Q500 ultrasonic
processor (Qsonica, Newtown, CT) with a 1/4 in. microtip probe. A
Thermo Evolution 350 UV–vis spectrometer (Thermo Fisher Scientific,
Waltham, MA) was used to quantify the metal salt leakage from the
aqueous emulsion phase to the continuous DCE phase. Scanning electron
microscopy (SEM) images and EDX spectra were acquired using a Nanoscience
Phenom Pro instrument (Nanoscience, Phoenix, AZ). ICP–MS data
were collected using a Nexion 350D (PerkinElmer, Waltham, MA).

Laber C.H., Scircle A.R., Mouton Z.P., Thornell T., Antony A., Jurss J.W, & Glasscott M.W. (2023). Tunable High Entropy Lanthanide Oxide Microspheres via Confined Electroprecipitation in Emulsion Droplet Scaffolds. ACS Materials Au, 4(2), 179-184.

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Graphite Oxidation via Modified Hummers Method

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Oxidation of graphite was carried out according to the modified Hummers method.^{3 (link)} Briefly, graphite powder (0.25 g) was suspended in sulfuric acid (H₂SO₄, 15 mL) and was kept in an ice bath for 25 min with stirring. Subsequently, sodium nitrate (NaNO₃, 0.25 g) was added to the suspension and the mixture was stirred for 60 min. Then, potassium permanganate (1.2 g) was slowly added, maintaining a temperature of 35 °C for 60 min. Later, 50 mL of water was added, and then hydrogen peroxide (5 mL, 30 vol%) was slowly added to the reaction mixture with stirring for 30 min. In this step, an exothermic effect was produced with the mixture reaching a temperature ∼73 °C, which was then cooled in an ice bath. Finally, the samples were washed using water and several centrifugation cycles were applied (2422 × g, 10 min at 25 °C).
For the complete exfoliation of GO, the resultant suspension (20 mL) was sonicated in an ice bath (Q500 ultrasonic processor, 500 watts, QSONICA, 30 pulses, 35% amplitude dial setting) for 15 min. Then, the suspension was centrifuged (2422 × g, 10 min at 25 °C), and the precipitate was dehydrated at 60 °C for 7 days.

Diaz-Galvez K.R., Teran-Saavedra N.G., Burgara-Estrella A.J., Fernandez-Quiroz D., Silva-Campa E., Acosta-Elias M., Sarabia-Sainz H.M., Pedroza-Montero M.R, & Sarabia-Sainz J.A. (2019). Specific capture of glycosylated graphene oxide by an asialoglycoprotein receptor: a strategic approach for liver-targeting. RSC Advances, 9(18), 9899-9906.

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Cellulose-based Hydrogel Fabrication

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Hydrogels were fabricated based on cerium-initiated graft copolymerization by employing ceric ions to initiate polymerization of acrylamide grafted from the cellulose particle backbone. CNC:CNF mixture suspensions were prepared by mixing CNC and CNF suspensions (3 wt% each) at 1:3, 1:1, and 3:1 (wt/wt) CNC to CNF and homogenized by sonication using a Q500 ultrasonic processor (Qsonica, Newtown, CT, USA) at 80% amplitude 1 s on/1 s off pulse regime for a total of 30 min. The CNC:CNF solutions were then diluted to 1.6 wt% with AM (0.07 mol) and BAM (0.03 mmol) to a total volume of 20 mL, as is suggested by the protocol described by Weng et al. [47 (link)]. Next, an initiator solution was prepared by dissolving CAN (0.036 mmol) in 5 mL double distilled water and adjusting the pH to 1 using 1 M HCl. The initiator solution was added dropwise to the cellulose-acrylamide mixture by stirring and poured into molds. Polymerization occurred at 50 °C for 12 h. Fabricated hydrogels were carefully removed for further testing. The resulting series of compositions were labeled according to their CNC to CNF content ratio, and categorized, namely C1F3 (1:3), C1F1 (1:1), C3F1(3:1), C1F0 (1:0), and C0F1 (0:1), where C and F represent CNC and CNF, respectively.

Rudich A., Sapru S, & Shoseyov O. (2023). Biocompatible, Resilient, and Tough Nanocellulose Tunable Hydrogels. Nanomaterials, 13(5), 853.

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Isolation and Quantification of Soluble Proteins

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To isolate soluble protein, cell monolayers were scraped into radio immunoprecipitation assay or RIPA buffer (Sigma Aldrich) with protease and phosphatase inhibitors (Thermo Scientific), and then sonicated using a Q500 ultrasonic processor (QSonica). Lysates were then rocked at 4°C for 15 minutes, and centrifuged at 12,500 × g for 15 minutes at 4°C to remove insoluble protein. Total soluble protein isolated in the supernatant was quantified using a bicinchoninic acid assay and a Nanodrop 2000c spectrophotometer (both from Thermo Scientific).

Mangold C.A., Yao P.J., Du M., Freeman W.M., Benkovic S.J, & Szpara M.L. (2018). Expression of the purine biosynthetic enzyme phosphoribosyl formylglycinamidine synthase (FGAMS) in neurons. Journal of neurochemistry, 144(6), 723-735.

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