The TAC concentration levels in the plasma were assessed using the ELISA assay kits (ZellBio GmbH, Germany) in accordance with the manufacturer's instructions. The TAC concentration assays can be used to determine the TAC concentration within a range of 0.125-2 mM with 0.1 mM sensitivity, intraassay CV 3.4%, and interassay CV 4.2%. MDA assessment in uterine and ovarian tissues was performed using ELISA assay kit (ZellBio GmbH, Germany). The assay detects the MDA level calorimetrically in a range of 0.78-50 µM with 0.1 µM sensitivity, intraassay CV 5.8%, and interassay CV7.6%.
Elisa assay kit
Manufactured by ZellBio
Sourced in Germany
The ELISA assay kit is a laboratory equipment used for enzyme-linked immunosorbent assay (ELISA), a widely used analytical technique for the detection and quantification of various biomolecules, such as proteins, antibodies, hormones, and cytokines, in a sample. The kit contains the necessary components, including microplates, reagents, and protocols, to perform ELISA experiments.
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Lab products found in correlation
3 protocols using elisa assay kit
1
Total Antioxidant Capacity and MDA Quantification
2
Hormonal and Antioxidant Assessment in Study
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The plasma levels of insulin, FSH, LH, and testosterone levels were measured by ELISA kits (DRG Instruments, Marburg, Germany). The hormone detection sensitivity was 1.27 mIU/mL for FSH, 0.856 mIU/mL for LH, and 0.287 nmol/L for testosterone. Glucose assessment was performed using biochemical assay kits (Pars Azmoon, Tehran, Iran), with a detection limit of 0.182 μIU/mL in each test tube. SOD (Randox Laboratories, Crumlin, UK) and MDA testing were performed using an ELISA assay kit (ZellBio, Lonsee, Germany). The assay detects the MDA level calorimetrically in a range of 0.78–50 µM with 0.1 µM sensitivity.
3
Metabolic Biomarkers in Surgical Patients
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Weight and height were measured with the precision of 0.1 kg and 0.1 cm, respectively. BMI was calculated as the weight in kilograms divided by the square of the height into meters (kg/m 2 ). Waist circumference was measured at the level of the umbilicus over light clothing using unstretched tape with no pressure to the body's surface; measurements were recorded to the nearest 0.1 cm. Systolic and diastolic blood pressures were measured twice in the sitting position after at least 10 min of rest, and the average was considered.
Before the surgery, 5 mL of blood (after 10-12 h fasting) was obtained from all participants. To measure fasting plasma glucose (FPG), the glucose oxidase method was applied. Enzymatic methods measured TG and total cholesterol (TC). Intra-and inter-assay coefficients of variation were <1% and <2.1%, respectively. Commercial kits, Pars Azmoon Co. (Tehran, Iran), were used to measure FPG, TG, and TC. Furthermore, the ELISA method determined the insulin level using Mercodia kits (Uppsala, Sweden). The ELISA assay kit determined the Apelin plasma levels (ZellBio, Ulm, Germany). Inter-and intra-assay CVs were 1.7-2.3 and 1.9% for both, respectively.
Before the surgery, 5 mL of blood (after 10-12 h fasting) was obtained from all participants. To measure fasting plasma glucose (FPG), the glucose oxidase method was applied. Enzymatic methods measured TG and total cholesterol (TC). Intra-and inter-assay coefficients of variation were <1% and <2.1%, respectively. Commercial kits, Pars Azmoon Co. (Tehran, Iran), were used to measure FPG, TG, and TC. Furthermore, the ELISA method determined the insulin level using Mercodia kits (Uppsala, Sweden). The ELISA assay kit determined the Apelin plasma levels (ZellBio, Ulm, Germany). Inter-and intra-assay CVs were 1.7-2.3 and 1.9% for both, respectively.
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