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Twist antibody

Manufactured by Santa Cruz Biotechnology
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The Twist antibody is a laboratory reagent designed for use in various immunoassay techniques. It functions as a tool for the detection and quantification of target proteins or antigens in biological samples.

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Lab products found in correlation

N myc, by Cell Signaling Technology (1 mentions) Nupage novex 4 12 bis tris gel, by Thermo Fisher Scientific (1 mentions) Anti β actin monoclonal antibody, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Horseradish peroxidase hrp conjugated secondary antibodies against mouse and rabbit igg, by Santa Cruz Biotechnology (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Cleaved parp, by Cell Signaling Technology (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Cell death detection elisaplus, by Roche (1 mentions) Seaplaque agarose, by Lonza (1 mentions) Opti mem reduced serum medium, by Thermo Fisher Scientific (1 mentions) Vimentin antibody, by Santa Cruz Biotechnology (1 mentions) Vegf antibody, by Santa Cruz Biotechnology (1 mentions) E cadherin antibody, by Santa Cruz Biotechnology (1 mentions) Thermoscript rt pcr system, by Thermo Fisher Scientific (1 mentions) Groα elisa kit, by R&D Systems (1 mentions) Pd98059, by Cell Signaling Technology (1 mentions) N cadherin antibody, by Santa Cruz Biotechnology (1 mentions) Slug antibody, by Abcam (1 mentions) Mouse β actin antibody, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Twist (57 citations) Anti-twist antibody (3 citations)

4 protocols using twist antibody

1

Antibody Characterization for Cell Signaling

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Antibody against CDC42 was purchased from Cytoskeleton, Inc. (Denver, CO) and Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Antibody against AKT2, AKT, survivin, N-myc, cleaved caspase-3, cleaved PARP and cell lysis buffer were obtained from Cell Signaling Technology (Beverly, MA). Twist antibody and horseradish peroxidase (HRP)-conjugated secondary antibodies against mouse and rabbit IgG were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Anti β-actin monoclonal antibody and fetal bovine serum (FBS) were from Sigma-Aldrich (St. Louis, MO). NuPAGE Novex 4–12% Bis–Tris Gel and Lipofectamine 2000 were purchased from Invitrogen (Carlsbad, CA). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies, Inc. (Santa Clara, CA). Agarose (SeaPlaque®) was from Lonza Inc. (Allendale, NJ). Cell Death Detection ELISAPlus was purchased from Roche Applied Science (Indianapolis, IN).
Lee S., Craig B.T., Romain C.V., Qiao J, & Chung D.H. (2014). Silencing of CDC42 inhibits neuroblastoma cell proliferation and transformation. Cancer letters, 355(2), 210-216.
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2

Regulation of EMT by GROα in Tumor Cells

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Materials were purchased from various manufacturers: recombinant GROα (R&D Systems), GROα siRNA (Santa Cruz Biotechnology), control siRNA (Santa Cruz Biotechnology), Lipofectamine ltx (Invitrogen), Opti-MEM Reduced Serum medium (Invitrogen), GROα ELISA kit (R&D Systems), GROα primers (IDT), ThermoScript™ RT-PCR systems (Invitrogen), SYBR Green (Qiagen), EMT marker primers (IDT), PD98059 (Cell Signaling), E-cadherin antibody (Santa Cruz Biotechnology), N-cadherin antibody (Santa Cruz Biotechnology), Snail antibody (Abcam), Slug antibody (Abcam), Twist antibody (Santa Cruz Biotechnology), Vimentin antibody (Santa Cruz Biotechnology), VEGF antibody (Santa Cruz Biotechnology), phospho-MAPK antibody (Cell Signaling), MAPK antibody (Cell Signaling), β-actin mouse antibody (Santa Cruz Biotechnology).
Bhat K., Sarkissyan M., Wu Y, & Vadgama J.V. (2017). GROα overexpression drives cell migration and invasion in triple negative breast cancer cells. Oncology Reports, 38(1), 21-30.
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3

Multivariate Immunohistochemical Analysis

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The antibodies against IDO1, cytokeratin 8 (CK8), cytokeratin 18 (CK18), fibronectin, and SV40 Large T antigen were from Abcam (Cambridge, UK). The β-actin antibody and the epithelial cell adhesion molecule (EpCAM) antibody were from Sigma–Aldrich (St. Louis, MI). The E-Cadherin and Vimentin antibodies were from Cell Signaling Technology (Danvers, MA). The twist antibody was from Santa Cruz Biotechnology (Santa Cruz, CA); The fluorescin-conjugated antibodies for detecting CD8 (FITC), IFNγ (PE), CD3 (APC), CD14 (FITC), and CD206 (APC) were from BD Biosciences (San Diego, CA).
Han X., Na T., Wu T, & Yuan B.Z. (2020). Human lung epithelial BEAS-2B cells exhibit characteristics of mesenchymal stem cells. PLoS ONE, 15(1), e0227174.
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4

Antibody Procurement for Protein Analysis

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MG132 (#C2211) was purchased from Sigma (St. Louis, MO, USA). Beta-actin antibody was purchased from Sungene Biotech (Tianjin, China) clone KM9001. Beta-catenin and GAPDH antibodies were purchased from Proteintech and Utibody, respectively (10366-1-AP and UM4002, respectively). TWIST antibody was purchased from Santa Cruz (sc-81417). Ubiquitin K48 antibody was purchased from ZENBIO (R24785).
Liu J., Shu G., Wu A., Zhang X., Zhou Z., Alvero A.B., Mor G, & Yin G. (2022). TWIST1 induces proteasomal degradation of β-catenin during the differentiation of ovarian cancer stem-like cells. Scientific Reports, 12, 15650.
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