The MSAP experiment comprised two digestion reactions, the first digestion reaction included 16 U of EcoRI (TaKaRa, Dalian, China) plus 10 U of MspI (TaKaRa), the second digestion reaction was the same as the first digestion except the HpaII (TaKaRa) instead of MspI. The MSAP pre-amplification and selective amplification reaction conditions and the method of electrophoresis were followed by Cao et al. [26] . The MSAP adapter and selective amplification primer sequences are listed in
Hpaii
HpaII is a type II restriction endonuclease that recognizes and cleaves the DNA sequence 5'-CCGG-3'. It is commonly used in molecular biology applications, such as DNA digestion and fragment analysis.
Lab products found in correlation
7 protocols using hpaii
Amplified Fragment Length Polymorphism and Methylation-Sensitive Amplification Polymorphism Protocols
The MSAP experiment comprised two digestion reactions, the first digestion reaction included 16 U of EcoRI (TaKaRa, Dalian, China) plus 10 U of MspI (TaKaRa), the second digestion reaction was the same as the first digestion except the HpaII (TaKaRa) instead of MspI. The MSAP pre-amplification and selective amplification reaction conditions and the method of electrophoresis were followed by Cao et al. [26] . The MSAP adapter and selective amplification primer sequences are listed in
X-Chromosome Inactivation Pattern Analysis
Histochemical Staining with DAB and DAPI
Methylation Analysis of AR Gene
Genomic DNA Extraction and Methylation Analysis
Immunochemical Detection of DNA Modifications
FRAXA Locus Methylation Assay for XCI Analysis
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