Western blotting was conducted as previously described.19 (link),20 (link) Briefly, the total proteins were extracted from the cells with RIPA lysis buffer, separated in 8–12% SDS-PAGE gels, and transferred onto PVDF (polyvinylidene fluoride) membranes (Millipore, Billerica, MA, USA). After blocking with 5% nonfat milk at room temperature for 1 hour, the membranes were incubated with the relevant primary antibodies followed by an appropriate secondary antibody. The primary antibodies in the current study included anti-UBE2O (1:800, Abcam), anti-E-cadherin, anti-Vimentin, anti-N-cadherin, anti-Snail, anti-Zeb1 and anti-Zeb2 (1:800, Cell Signaling Technology, Danvers, MA, USA). Anti-β-Actin (1:1000, Beyotime, Shanghai, China) was used as a loading control. The blots were visualized using ECL reagents (Pierce, Rockford, IL, USA) and a chemiluminescence imaging system (Bio-Rad, Richmond, CA, USA).
Anti ube2o
Manufactured by Abcam
Sourced in United States
Anti-UBE2O is a laboratory reagent that specifically binds and detects the UBE2O protein. UBE2O is an enzyme involved in protein ubiquitination, a process that tags proteins for degradation. Anti-UBE2O can be used to study the expression and localization of UBE2O in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti zeb2, by Cell Signaling Technology (1 mentions)
Anti zeb1, by Cell Signaling Technology (1 mentions)
Polyvinylidene fluoride (pvdf), by Merck Group (1 mentions)
Anti n cadherin, by Cell Signaling Technology (1 mentions)
Anti β actin, by Beyotime (1 mentions)
Anti snail, by Cell Signaling Technology (1 mentions)
Anti vimentin, by Cell Signaling Technology (1 mentions)
Anti e cadherin, by Abcam (1 mentions)
Chemiluminescence imaging system, by Bio-Rad (1 mentions)
Anti cyclin d1, by Abcam (1 mentions)
Anti calnexin, by Abcam (1 mentions)
Anti tsg101, by Abcam (1 mentions)
Anti cd81, by Abcam (1 mentions)
2 protocols using anti ube2o
1
Western Blotting of EMT Markers
2
Western Blot Analysis of Salivary Exosomes
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Total protein was extracted from saliva, saliva-Exos and cells. Then 40 μg proteins of each sample was separated by 10% SDS-PAGE, and transferred onto PVDF membranes. The membranes were incubated with primary antibodies at 4 °C for overnight, and with horseradish-peroxidase-conjugated secondary antibodies at 37 °C for 1 h. The following antibodies were used: anti-CD81 (1:1000, Abcam, MA, USA, #ab109201), anti-TSG101 (1:1000, Abcam, MA, USA, #ab125011), anti-Calnexin, (1:1000, Abcam, MA, USA, #ab125011), anti-Cyclin D1 (1:1000, Abcam, MA, USA, #ab40754), anti-Cyclin D3 (1:1000, CST, USA, #2936), anti-UBE2O (1:500, Abcam, MA, USA, #ab254592), anti-SMAD6 (1:1000, Abcam, MA, USA, #ab80049), anti-BMP2 (1:1000, Abcam, MA, USA, #ab14933) and anti-GADPH (1:10,000, Abcam, USA, #ab37168).
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