Tuna skin was supplied from Ningbo Today Food Co. Ltd. (Zhejiang, China). Glacial acetic acid, pepsin, sodium chloride, chitosan, glycerol, sodium bromide and LB medium were obtained from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Escherichia coli CICC 20658, Bacillus subtilis CICC 10732, Staphylococcus aureus CICC 10,201 and Listeria monocytogenes ATCC 19,111 were ordered from China Center of Industrial Culture Collection (Beijing, China). 1,1-diphenyl-2-picryl-hydrazyl (DPPH) was purchased from Sigma Company (St. Louis, MO, USA). All reagents were of analytical grade.
Pepsin
Manufactured by Sinopharm
Sourced in China
Pepsin is a proteolytic enzyme produced by the stomach that plays a key role in the digestion of proteins. It is an aspartic protease that functions optimally in the acidic environment of the stomach, where it helps break down and hydrolyze ingested proteins into smaller peptides and amino acids for further absorption.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin, by Sinopharm (6 mentions)
Sodium chloride (nacl), by Sinopharm (6 mentions)
Sodium hydroxide (naoh), by Sinopharm (6 mentions)
Pancreatin, by Sinopharm (5 mentions)
Hydrochloric acid (hcl), by Sinopharm (4 mentions)
Chitosan, by Sinopharm (3 mentions)
Escherichia coli cicc 20658, by China Center of Industrial Culture Collection (2 mentions)
Bacillus subtilis cicc 10732, by China Center of Industrial Culture Collection (2 mentions)
Staphylococcus aureus cicc 10201, by China Center of Industrial Culture Collection (2 mentions)
Glycerol, by Sinopharm (2 mentions)
Sodium bromide, by Sinopharm (2 mentions)
Alcalase, by Sinopharm (2 mentions)
Milli q system, by Merck Group (2 mentions)
Chitosan from shrimp shells, by Sinopharm (2 mentions)
Ferrous sulfate heptahydrate, by Sinopharm (2 mentions)
Ethanol, by Sinopharm (2 mentions)
Hplc grade acetonitrile, by Merck Group (2 mentions)
Acetic acid, by Sinopharm (2 mentions)
Lb medium, by Sinopharm (1 mentions)
1 1 diphenyl 2 picrylhydrazyl dpph, by Merck Group (1 mentions)
25 protocols using pepsin
1
Extraction and Characterization of Tuna Skin
2
Chicken Meat Protease Extraction Protocol
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Chicken meat was grinded by a high‐speed tissue homogenizer and kept in −18℃ freezer for further use. Chicken fat was obtained from Bewaga Foods Co., Ltd (China).
1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) was purchased from Sigma‐Aldrich (USA). Maltodextrin was obtained from Lihua Starch Co., Ltd (China). Papain (900,000 U/ g) and flavourzyme (120 U/g) were obtained from Guangzhou Huaqi Biotechnology Co., Ltd (China); neutral protease (1600 AU/g), exoprotease (500,000 HUT/g), and alkali protease (580,000 DU/g) were obtained from Genencor International Ltd. (USA); composite protease (1.5 AU/g) was obtained from NOVOZYMES (USA); pepsin (1200 U/g) was obtained from Shanghai Sinopharm Chemical Reagent Co., Ltd. (China); and trypsin (4000 U/g), bromelain (800,000 U/g), and acid protease (50,000 U/g) were obtained from Auspicious New Biological Pharmaceutical Co., Ltd. (China). All other chemicals and solvents used were of analytical grade.
1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) was purchased from Sigma‐Aldrich (USA). Maltodextrin was obtained from Lihua Starch Co., Ltd (China). Papain (900,000 U/ g) and flavourzyme (120 U/g) were obtained from Guangzhou Huaqi Biotechnology Co., Ltd (China); neutral protease (1600 AU/g), exoprotease (500,000 HUT/g), and alkali protease (580,000 DU/g) were obtained from Genencor International Ltd. (USA); composite protease (1.5 AU/g) was obtained from NOVOZYMES (USA); pepsin (1200 U/g) was obtained from Shanghai Sinopharm Chemical Reagent Co., Ltd. (China); and trypsin (4000 U/g), bromelain (800,000 U/g), and acid protease (50,000 U/g) were obtained from Auspicious New Biological Pharmaceutical Co., Ltd. (China). All other chemicals and solvents used were of analytical grade.
3
Trace Element Analysis of Guangxi Rice
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Rice from Guangxi Province, one of China’s major grain-producing areas, was selected as the object of our study.
Pepsin (catalogue No. 64007137) and pancreatin (catalogue No. 64006737) were purchased from Sinopharm Chemical Reagent Co., Ltd., China, and bile extract (porcine; catalogue No. B822609) was purchased from Macklin, Shanghai, China. All the organic acids used, including citric acid, malic acid, lactic acid and acetic acid, were of analytical purity.
Dietary supplements (vitamin B6 (VB6), vitamin C (VC), vitamin E (VE), folic acid (VB9), niacin (VB3), copper (Cu), methionine (Met) and coenzyme Q10 (Q10) and procyanidins (OPC) were of an excellent level of purity and were purchased from Macklin reagent company.
High-purity deionized water from a Milli-Q Plus system (Millipore, Bedford, MA, USA) was used for dilutions and washes. Element concentrations were determined by inductively coupled plasma optical emission spectrometry (ICP-MS) on an Elan DRC-e system (PerkinElmer, Massachusetts, USA), whose limits of detection (LODs) were 0.1–1 ng g−1 for Se and Zn and less than 0.1 ng g−1 for Cd.
Pepsin (catalogue No. 64007137) and pancreatin (catalogue No. 64006737) were purchased from Sinopharm Chemical Reagent Co., Ltd., China, and bile extract (porcine; catalogue No. B822609) was purchased from Macklin, Shanghai, China. All the organic acids used, including citric acid, malic acid, lactic acid and acetic acid, were of analytical purity.
Dietary supplements (vitamin B6 (VB6), vitamin C (VC), vitamin E (VE), folic acid (VB9), niacin (VB3), copper (Cu), methionine (Met) and coenzyme Q10 (Q10) and procyanidins (OPC) were of an excellent level of purity and were purchased from Macklin reagent company.
High-purity deionized water from a Milli-Q Plus system (Millipore, Bedford, MA, USA) was used for dilutions and washes. Element concentrations were determined by inductively coupled plasma optical emission spectrometry (ICP-MS) on an Elan DRC-e system (PerkinElmer, Massachusetts, USA), whose limits of detection (LODs) were 0.1–1 ng g−1 for Se and Zn and less than 0.1 ng g−1 for Cd.
4
Enzymatic Assays for Oxidative Stress
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Fluorescein diacetate (FDA), propionyl iodide (PI), sodium dodecyl sulfate, acrylamide, and methylenebisacrylamide were provided by Suo Lai Bao Biotechnology Co., Ltd. Pepsin, papain, trypsin, and amylase were provided by Sinopharm Group Chemical Reagent Co., Ltd., Malondialdehyde (MDA), superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) reagent test kits were provided by Nanjing Jiancheng Biological Engineering Research Institute.
5
Silica-Based Hemoglobin Immobilization
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Silica clay was obtained from Ningbo Create New Materials Co., (Ningbo, China). Pepsin was purchased from Sinopharm Reagent Co. (Shanghai, China). 3-Aminopropyltriethoxysilane (APTES), glutaraldehyde, and hemoglobin were obtained from Aladdin Biochemical Technology Co (Shanghai, China). All other reagents were analytical grade reagents and used without further purification.
6
Protein Content Analysis of False Abalone
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Fresh false abalone were purchased from the local market in Dalian, China. The water content and protein content of the false abalone samples were 77.53 ± 2.13% and 18.24 ± 1.03% using methods GB/T5009.3-2016 and GB/T5009.5-2016, respectively. Five proteases including pepsin (≥400 units/mg protein), alcalase (≥200 units/mg protein), trypsin (≥2500 USP units/mg protein), neutrase (≥600 units/mg protein), and flavourzyme (≥300 units/mg protein) were purchased from Sinopharm Chemical Reagent Co., Ltd., Shanghai, China. Other chemicals and reagents were of analytical degree.
7
In Vitro Evaluation of Proton Pump Inhibitors
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Esomeprazole magnesium enteric coated tablets (Nexium®) were purchased from AstraZeneca AB (Wuxi, China, batch number: 2006122). Omeprazole magnesium enteric coated tablets (Losec® multiple unit pellet system) were purchased from AstraZeneca AB (SE-151 85, Södertälje Swedish, batch number: 1912139). Pepsin was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Glass microspheres (GM, 30 mesh, with a diameter of 600 μm) were obtained from Ningbo Xinou Sandblasting Machinery Co., Ltd. (Ningbo, China). Precise pH test paper was obtained from Shanghai Macklin Biotechnology Co., Ltd. (Shanghai, China). Sprague–Dawley rats (180–220 g, IACUC Application No. 2018-05-ZJW-18) were supplied from Shanghai Laboratory Animal Center, Chinese Academy of Sciences (Shanghai, China). Hydrochloric acid, sodium dihydrogen phosphate, sodium hydrogen phosphate, sodium hydroxide, ethanol, methanol and acetonitrile of analytical grade were obtained from Sinopharm Chemical Reagent Co., Ltd. (China). Water was purified by a reverse osmosis using Milli-Qs system (Millipore, Bedford, MA, USA).
8
Fuji Apple Preservation with Antimicrobials
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Plate Count Agar (PCA), Rose Bengal Agar (RBA), Man Rogosa Sharpe (MRS) broth, pepsin, bile extract, and dextran (DX) of 20 kDa molecular weight were purchased from Sinopharm Chemical Reagent Co., ltd. (Shanghai, China). Whey protein isolate (WPI) was purchased from Hilmar Ingredients (California, USA). Eugenol was purchased from Aladdin (Shanghai, China). Fresh mature ‘Fuji’ apples (harvested in the fall of 2021) were purchased from a supermarket in Shaanxi province.
9
Insulin Delivery Platform Development
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Insulin was purchased from Wanbang Jinqiao Pharmaceutical Co., Ltd. (Xuzhou, Jiangsu, China). β-CD was obtained from Anhui Sunhere pharmaceutical excipients Co., Ltd. (Anhui, China). SDS was purchased from Sigma-Aldrich Company (St. Louis, MO, USA). Pepsin (enzyme activity ≥1200 U/g), trypsin (enzyme activity ≥2500 U/mg) and β-GP (disodium salt pentahydrate) were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Chitosan (molecular weight about 1000 kD) was purchased from Zhejiang Golden-Shell biological chemical Co., Ltd. (Taizhou, Zhejiang, China). Acetonitrile (HPLC grade) was obtained from Merck Co., Ltd. (Darmstadt, Germany). Other reagents were of analytical grade. Water was purified by Milli-Q system (Millipore, Bedford, MA, USA).
10
Mg-2Zn Wires: Fabrication and Characterization
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Mg-2Zn (wt.%, the chemical composition is shown in Table 1 ) wires, developed by our research group [[37] (link), [38] (link), [39] (link)] and produced by Suzhou Jingjun New Materials Technology Co. Ltd. China, with a diameter of 1.0 mm were used in this study. The experimental specimens were first ultrasonically cleaned for 5 min in acetone, then polished in a solution consisting of 1 ml nitric acid, 1 ml hydrogen peroxide, and 100 ml distilled water for 1 min. Finally, they were cleaned for 5 min in ethanol ultrasonically and dried under the excitation of UV light in the biosafety cabinet. The surface morphology and microstructure of the wire were observed by a Sirion200 field emission scanning electron microscope (SEM, FEI, Japan) with an acceleration voltage of 20 kV and spot size of 4, and optical metallographic microscopy (BX60 M, Olympus, Japan) as shown in Fig. S1 , from which it is clear that original wire surface is smooth and uniform. For preparing the various solutions, hydrochloric acid, sodium hydroxide, potassium dihydrogen phosphate, pepsin, and pancreatin were purchased from Sinopharm Chemical Reagent Co., Ltd. China.Table 1
Chemical composition of Mg-2Zn.
| Composition（wt.%） | Zn | Ca | Al | Mn | Si | Cu | Fe | Ni | Mg |
|---|---|---|---|---|---|---|---|---|---|
| Mg-2Zn | 2.3033 | 0.0032 | 0.0407 | 0.0250 | 0.0289 | 0.0008 | 0.0079 | 0.0024 | Bal |
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