Traceselect water

DOTA-D-Phe¹-Tyr³-octreotide (DOTATOC) was obtained from ABX (Radeberg, Germany) and an aqueous stock solution of 1 mg/mL was prepared. All chemicals were of pure chemical grade and solvents for HPLC were obtained as HPLC grade. TraceSelect water (Sigma-Aldrich, Germany) was used in all experiments. Labeling reactions were incubated in a ThermoMixer MHR 11 (DITABIS, Digital Biomedical Imaging Systems AG).
Activity counting was determined using a borehole counter (Nuklear-Medizintechnik Dresden GmbH, Germany). RadioTLC was performed using aluminum-backed silica gel (silica-gel 60 F254; Merck, Darmstadt, Germany) and analyzed using a flatbed-scanner (Instant Imager®, Packard Canberra, Schwadorf, Austria). HPLC was performed using Hitachi L-7100 pump system coupled with UV (Hitachi L-7400) and radiometric (Gaby Star, Raytest Isotopenmessgeräte, GmbH, Straubenhardt, Germany) detectors.

The fibronectin-binding peptide (FnBPA5) and its scrambled control peptide (scrambled FnBPA5) were purchased from Peptide Specialty Laboratories GmbH (Heidelberg, Germany) conjugated with a maleimide NODAGA. The compounds were dissolved in TraceSELECT^® Water (Sigma-Aldrich, Buchs, Switzerland) to a final concentration of 0.25 mM. 14 nmol of each peptide were radiolabeled in 0.5 M ammonium acetate pH 5.5 by adding 80 MBq ¹¹¹InCl₃ (Mallinckrodt, Wollerau, Switzerland) followed by a 30 min incubation step at 50 °C. Quality control was performed by reversed-phase HPLC (Varian Prostar HPLC System, SpectraLab Scientific Inc., Canada); column Dr.Maisch Reprospher 300 C18-TN, 4.6 cm × 150 mm; 5 μm The column was eluted with acetonitrile containing 0.1% TFA and Ultrapure water containing 0.1% TFA and a linear gradient starting with 15% acetonitrile up to 95% over 15 min with a flow rate of 1 mL per minute.

PentixaFor was obtained from PentixaPharm. An aqueous stock solution of 1 mg/mL was prepared and kept at − 15 °C. All chemicals were of pure chemical grade and solvents for high-pressure-liquid-chromatography (HPLC) were obtained as HPLC grade. TraceSelect water (Sigma-Aldrich) was used in all experiments. The pharmaceutical grade ⁶⁸Ge/⁶⁸Ga generator (GalliaPharm®, Eckert & Ziegler Radiopharma GmbH, Germany), Modular Lab PharmTracer (Eckert & Ziegler Eurotope GmbH, Germany) and reagent set EZ-102 (Eckert & Ziegler Eurotope GmbH, Germany) were used. The amount of detected metal impurities/ ⁶⁸Ge breakthrough as provided by the manufacturer was less than the defined limit in the European Pharmacopeia monograph. Activity counting was determined using a borehole counter (Nuklear-Medizintechnik Dresden GmbH, Germany). HPLC was performed using the HPLC system Knauer Azura (UVD: 2.1 L; P6.1L) coupled with UV and radiometric (Raytest Socket 2″8103 0370) detectors. The TLC scanner used was MiniGita from Raytest. The test for endotoxins was performed using Nexgen PTS (Charles River).