Tetrahydrofuran hplc grade

Tween-80 was purchased from Nanjing Well Pharmaceutical co., LTD. (Nanjing, China). Ethanol (AR grade), phosphate acid (AR grade), ethyl acetate (AR grade), hydrochloric acid (AR grade), n-hexane (AR grade) were all purchased from Sinopharm Chemical Reagent Co. LTD. (Shanghai, China). Standards of ginkgolide A ≥ 95%, ginkgolide B ≥ 95%, ginkgolide C ≥ 95%, ginkgolide J ≥ 95%, bilobalide ≥ 98%, quercetin ≥ 98%, isorhamnetin ≥ 98%, kaempferol ≥ 98% were purchased from National Institutes for Food and Drug Control (Beijing, China). Formic acid (MS grade), acetonitrile (HPLC grade), triffuoroacetic acid (HPLC grade), tetrahydrofuran (HPLC grade) and methanol (HPLC grade) were acquired from Merck (Darmstadt, Germany). Fetal Bovine Serum (FBS) was obtained from GIBCO (Australia Origin). DME/F12, PBS buffer, Trypsin, and Penicillin-streptomycin were purchased from Hyclone (Logan, Utah, United States). Anti-CD90-PerCP, anti-CD45-FITC, anti-CD29-PE and anti-CD34-Alexa Fluor 647 were acquired from BD Biosciences (San Diego, United States).

PFV (95:5 mole ratio), BDMO-PPV, HPLC grade tetrahydrofuran (THF) and (3-Aminopropyl)- triethoxysilane (APTES) were purchased from Sigma-Aldrich (Milwankee, USA). PS-PEG-COOH was purchased from Polymer Source Inc. Ltd., (Dorval, QC, Canada; Back bone PS Mn = 6500; each branch Mn = 4600; Mn total = 30 000, Mw/Mn = 1.3). Hydrochloric acid (AR, 36.5%) and sodium hydroxide (AR) were ordered from Guangzhou Chemical Reagent Factory (Guangzhou, China). Ultra-pure water used for all the experiments was prepared with a Milli-Q water system (Millipore, Bedford, MA, USA).