Cpccoet

Reagents were purchased from Sigma except for GABAzine (SR95531, Tocris#1262), CGP55845 (Tocris#1248), D-AP5 (Tocris#0106), DNQX (Tocris#2312), Noradrenaline (Abcam#ab120717), HEAT (Tocris#0535), Phenylephrine (Tocris#2838), Isoproterenol (Tocris#1747), Win (Tocris#1038), AM251 (Tocris#1117), Rimonabant (Tocris#0923), MPEP (Tocris#1212), CPCCOEt (Tocris#1028), CNO (Hello Bio#HB6149), Cy5-streptavidin (ThermoFisher#434316). For i.p. injection, Rimonabant (Tocris #0923) was first prepared 30 mg/mL stock in DMSO, then diluted in saline with 1% DMSO, 1% Cremophor, 1% Ethanol to make 0.3 mg/mL for injection, finally injected 0.01 mL/g to reach 3 mg/kg injection. Phenylephrine (Tocris #2838) was prepared at 10 mM stock solution in water, then diluted in saline for injection. CNO (HelloBio #HB6149) was prepared 0.1 mg/mL in saline and injected 0.01 mL/1 g to reach 1 mg/kg injection. For slice recordings, all stocks were diluted to final concentrations in ACSF to achieve a final solvent concentration of less than 0.1% v/v

Neurobasal (NB) media, 2 M KCl, B27 supplement, L-glutamine, and gentamicin were purchased from Life Technologies (Carlsbad, CA, USA). Receptor agonists (glutamate, aspartate, quisqualate, 3,5-dihydroxyphenylglycine (DHPG), (1S,3R)-1- aminocyclopentane-1,3-dicarboxylic acid (ACPD), N-methyl-D-aspartate (NMDA)), receptor antagonists (YM298198-HCl, CPCCOEt, JNJ16259685, MPEP, MK801, CFM2, MCCG, MAP4) were purchased from Tocris Bioscience (Bristol, United Kingdom). All receptor agonists were prepared in equimolar sodium hydroxide (VWR, Radnor, PA, USA) and adjusted to pH 7.3 – 7.5. All antagonists were diluted in DMSO (Fisher Scientific, Pittsburgh, PA, USA), except for MPEP, which was prepared in water. glutamate pyruvate transaminase (GPT) was obtained from Roche (Indianapolis, IN, USA). All other chemicals were purchased from Sigma (St. Louis, MO, USA).