Fitc conjugated goat anti mouse igg

Glass slides were placed in a 24‐well plate. Then, 250 μL of the cell suspension with the appropriate concentration was evenly spread on the slides. Cells were fixed with 4% paraformaldehyde (in PBS) for 20 minutes and permeabilized with 0.5% Triton X‐100 (in PBS) for 10 minutes. Goat serum was added to the slides for 1 hour at 25°C. Cells were incubated with mouse polyclonal anti‐STAT3 (1:100; ProteinTech) and placed in an incubator with high humidity at 4°C overnight. On the second day, the cells were incubated for 2 hour at room temperature with fluorescein (FITC)‐conjugated goat anti‐mouse IgG (ZSGB‐BIO, Beijing, China). DAPI (Beyotime Biotechnology, Shanghai, China) was used to stain nuclei and Antifade Mounting Medium (Beyotime Biotechnology) was used to seal the slides. Then, images were obtained using a fluorescence microscope.

Salidroside was purchased from MedChemExpress (Monmouth Junction, NJ, USA) with a purity ≥ 98%. Collagen-related peptide (CRP) was prepared as previously described [56 (link)]. Thrombin (≥ 10 NIH units/vial) were from Chrono-log Corporation (Havertown, PA, USA). FITC-conjugated mouse anti-human CD41a and PAC-1 antibody were from BD Biosciences (San Jose, CA, USA) and BECTON DICKINSON (San Jose, CA, USA) respectively. PE-conjugated anti-human/mouse CD62p (P-Selectin) and anti-human Glycoprotein VI purified antibody were purchased from eBioscience (San Diego, CA, USA). FITC-conjugated anti-CD42b antibody was from Abcam (Cambridge, MA, USA). FITC-conjugated goat anti-mouse IgG was purchased from ZSGB-BIO (Beijing, China). β-actin antibody and anti-rabbit IgG (HRP-linked) antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). SB216763 (GSK-3β inhibitor) was purchased from ApexBio Technology.

Matrine was purchased from MedChemExpress (Monmouth Junction, NJ, United States) with a purity ≥98% and dissolved in saline. Collagen-related peptide (CRP) was prepared as previously described (Arthur et al., 2012 (link)). Collagen and thrombin (≥10 NIH units/vial) were from Chrono-log Corporation (Havertown, PA, United States). Fibrinogen and BSA (bovine serum albumin) were purchased from Sigma-Aldrich (St. Louis, MO, United States). Mepacrine (also known as Quinacrine) was from APExBIO (Boston, MA, United States). FITC-conjugated mouse anti-human CD41a and PAC-1 antibody were from BD Biosciences (San Jose, CA, United States). PE-labelled anti-human/mouse CD62p (P-Selectin) and anti-human Glycoprotein VI antibody were purchased from eBioscience (San Diego, CA, United States). Alexa Fluor-546-labelled phalloidin was purchased from Thermo Fisher Scientific (Waltham, MA, United States). FITC-conjugated goat anti-mouse IgG was purchased from ZSGB-BIO (Beijing, China). β-actin antibody and HRP-conjugated anti-rabbit IgG were purchased from Cell Signaling Technology (Danvers, MA, United States).