Fitc conjugated pna

Manufactured by Vector Laboratories

FITC-conjugated PNA is a fluorescently labeled lectin that binds to galactose-containing glycoconjugates. It is commonly used for the detection and localization of glycoconjugates in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Phycoerythrin conjugated anti cd150 slamf1 clone tc15 12f12.2, by BioLegend (2 mentions) Fluorophore conjugated anti cd4, by Thermo Fisher Scientific (2 mentions) Anti mouse cxcr5, by BD (2 mentions) Phycoerythrin conjugated anti fas cd95 clone jo2, by BD (2 mentions) Allophycocyanin conjugated anti mouse bcl6 clone k112 91, by BD (2 mentions) Ikkε 2690, by Cell Signaling Technology (2 mentions) Igd clone 11 26, by Thermo Fisher Scientific (2 mentions) Clone okt3, by BioLegend (2 mentions) Icos clone c398.4a, by BioLegend (2 mentions) Rat anti mouse b220 clone ra3 6b2, by BD (1 mentions) Cy3 conjugated goat anti rat igg clone 112 165 143, by Jackson ImmunoResearch (1 mentions) Avidin biotin peroxidase technique, by Vector Laboratories (1 mentions) Bz image analysis software, by Keyence (1 mentions) Antifade solution, by Thermo Fisher Scientific (1 mentions) Bz 9000 fluorescence microscope, by Keyence (1 mentions)

Spelling variants of this product

PNA-FITC (25 citations) FITC-conjugated peanut agglutinin (PNA; (3 citations)

5 protocols using fitc conjugated pna

Retinal Wholemount and Cryosection Staining

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Enucleated eyes were fixed overnight in 4% paraformaldehyde (4°C) after removing the cornea from each eye. Retinal whole mounts or sections were prepared as described previously (Li et al., 2011 (link)). Sequentially, retinal whole mounts and cryosections were permeated with 0.1% Triton X-100, rinsed in 0.1 M PBS, and blocked in 5% bovine serum albumin (BSA). FITC-conjugated PNA (1:400; Vector Laboratories, Burlingame, CA) was used to identify the interphotoreceptor matrix sheath that surrounds the cone outer segments. The specimens were incubated in M-or S-opsin primary antibodies (1:400; Santa Cruz Biotechnology, Santa Cruz, CA) overnight at 4°C. The retinas were washed 3 times with PBS and incubated with IgG secondary antibody tagged with Alexa-594 (molecular Probes, Eugene, OR) diluted 1:500 in PBS at room temperature for 1 hour. The nuclei were stained with 4’, 6-diamidino-2- phenylindole. Retinal whole mounts and sections were mounted with coverslips for imaging by fluorescence microscopy. In retinal whole mounts, M-or S-opsins were manually counted 0.3 mm ventral to the optic nerve (Dai et al., 2016 (link)) within one field (53,652 μm²) at high magnification (*40).

Dai X., Pang S., Wang J., FitzMaurice B., Pang J, & Chang B. (2018). Photoreceptor degeneration in a new Cacna1f mutant mouse model. Experimental eye research, 179, 106-114.

+ Open protocol

+ Expand

Quantifying Allograft Vasculopathy and Germinal Centers

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Formalin-fixed hearts were paraffin mounted and stained using H&E and Weigert’s Elastin van Gieson method to delineate the internal elastic lamina and the severity of allograft vasculopathy assessed morphometrically, as reported previously (Motallebzadeh et al., 2012 (link)). Complement C4d deposition was assessed on 7-μm cryostat sections of donor heart allografts explanted after 50 days by an avidin-biotin-peroxidase technique (Vector Laboratories), using unconjugated rat anti-mouse C4 mAb (16D2; Abcam), as described previously (Win et al., 2009 (link)). GCs were quantified on 7-μm cryostat sections of recipient spleens harvested 50 days following transplant by immunofluorescence staining of B220⁺ B cells using rat anti-mouse B220 (clone RA3-6B2; BD Pharmingen) detected with Cy3-conjugated goat anti-rat IgG (clone 112-165-143, Jackson ImmunoResearch Laboratories) and peanut agglutinin (PNA)⁺ GC B cells using FITC-conjugated PNA (Vector Laboratories), as described previously (Conlon et al., 2012b (link)). Numbers of PNA⁺ GC were expressed as a percentage of total (B220⁺) lymphoid follicles.

Harper I.G., Ali J.M., Harper S.J., Wlodek E., Alsughayyir J., Negus M.C., Qureshi M.S., Motalleb-Zadeh R., Saeb-Parsy K., Bolton E.M., Bradley J.A., Clatworthy M.R., Conlon T.M, & Pettigrew G.J. (2016). Augmentation of Recipient Adaptive Alloimmunity by Donor Passenger Lymphocytes within the Transplant. Cell Reports, 15(6), 1214-1227.

+ Open protocol

+ Expand

Monoclonal Antibodies for Immune Cell Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Monoclonal Abs specific for human CD3 (clone OKT3), and mouse CD3 (clone 145-2C11), -CD28 (clone 37.51), -CTLA-4 (clone UC10-4B9) or -ICOS (clone C398.4A) were purchased from Biolegend CA, as was phycoerythrin-conjugated anti-CD150/SLAMf1 (clone TC15-12F12.2). Fluorophore-conjugated anti-CD4 (mouse: clone RM4-5; human: clone RPA-T4), -CD8a (clone 53-6.7), -CD19 (mouse: clone eBio1D3; human: clone HIB19), -CD25 (clone PC61), -CD40L (clone MR1), -CD44 (clone IM7), -CD45.1 (clone A20), -CD62L (clone MEL-14), -GL7 (clone GL-7), -IgD (clone 11-26) and -PD1 (mouse: clone J43; human: clone J105) Abs were obtained from eBioscience, CA. Anti-mouse CXCR5 (mouse: clone 2G8; human: clone RF8B2), biotinylated anti-CD138 (clone 281-2), FITC-conjugated TCR β-chain (H57-597), phycoerythrin-conjugated anti-Fas/CD95 (clone Jo2) and allophycocyanin-conjugated anti-mouse Bcl6 (clone K112-91) were procured from BD Biosciences, CA. FITC-conjugated PNA was purchased from Vector Laboratories, CA. Monoclonal anti-TBK1 (#3013), -phospho-TBK1 (Ser172) (clone D52C2, #5483), -TRAF2 (#4712), -TRAF3 (#4729), p-ERK1/2 (T202/Y204) (clone E10, #9106) and -IKKε (#2690) Abs were obtained from Cell Signaling Technology, MA. Monoclonal anti-p85α (sc-1637), -ERK2 (sc-1647) and -TRAF5 (sc-7220) were purchased from Santa Cruz Biotechnology, CA. Recombinant IL-2 and IL-7 cytokines were obtained from Biolegend, CA.

Pedros C., Zhang Y., Hu J.K., Choi Y.S., Canonigo-Balancio A.J., Yates JR I.I.I., Altman A., Crotty S, & Kong K.F. (2016). A TRAF-like motif of ICOS controls development of germinal center T follicular helper cells via TBK1. Nature immunology, 17(7), 825-833.

+ Open protocol

+ Expand

Retinal Cone Cell Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

All mice were maintained in accordance with Institutional Animal Care and Use Committee guidelines. Heterozygous Atf6^+/− (n = 4) and Atf6^−/− (n = 4) mice were sacrificed at 12 months of age, and retinal tissues were collected for retinal whole-mount imaging and molecular analyses. For retinal whole-mount studies, mouse eyes were fixed in 4% paraformaldehyde for 15 min. Eyes were dissected to isolate the retinas. Retinas were fixed in 4% paraformaldehyde for an additional 10 min, washed three times with PBS and incubated with FITC-conjugated PNA (1:50 dilution; Vector Laboratories) overnight at 4 °C. After several washes in PBS, the retinas were flat mounted and covered by a coverslip after the application of several drops of Antifade solution (Prolong, Invitrogen). Flat-mounted retinas were imaged using a Keyence BZ-9000 Fluorescence Microscope. PNA-positive cone cells were quantified with Keyence BZ image analysis software. The average number of cone cells per mm² with standard deviation was based on measurements from eight retinas. At least four fields were quantified per retina.

Kohl S., Zobor D., Chiang W.C., Weisschuh N., Staller J., Menendez I.G., Chang S., Beck S.C., Garrido M.G., Sothilingam V., Seeliger M.W., Stanzial F., Benedicenti F., Inzana F., Héon E., Vincent A., Beis J., Strom T.M., Rudolph G., Roosing S., den Hollander A.I., Cremers F.P., Lopez I., Ren H., Moore A.T., Webster A.R., Michaelides M., Koenekoop R.K., Zrenner E., Kaufman R.J., Tsang S.H., Wissinger B, & Lin J.H. (2015). Mutations in the unfolded protein response regulator ATF6 cause the cone dysfunction disorder achromatopsia. Nature genetics, 47(7), 757-765.

+ Open protocol

+ Expand

Monoclonal Antibodies for Immune Cell Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!