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Amaxa nucleofector

Manufactured by Horizon Discovery
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Sourced in United States

The Amaxa Nucleofector is a lab equipment product designed for the transfection of nucleic acids, such as DNA and RNA, into various cell types. It utilizes an electroporation-based technology to deliver the genetic material into the cells. The Nucleofector provides a platform for efficient and reliable transfection, enabling researchers to study gene expression, knockdown, or other cellular processes.

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Lab products found in correlation

Mouse macrophage nucleofector kit, by Lonza (1 mentions) Retromax, by Novus Biologicals (1 mentions) Image lab version 4, by Bio-Rad (1 mentions) On targetplus smartpool sirna, by Horizon Discovery (1 mentions) Chemidoc gel imaging system, by Bio-Rad (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Kgm gold bulletkit medium, by Cambrex (1 mentions) On targetplus smartpool, by Horizon Discovery (1 mentions)

4 protocols using amaxa nucleofector

1

Rab2a Depletion and Retroviral Transduction in Bone Marrow-Derived Macrophages

Cited 1 time
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Rab2a depletion in BMMs was carried out using a Mouse Macrophage Nucleofector kit (Lonza) and Amaxa Nucleofector with On-Targetplus SMARTpool siRNAs (Dharmacon) directed against mouse and human Rab2a (L-040851-01-0005 and L-010533-00-0005) or small interfering nontargeting (siNT; J-001810) siRNA, as described previously (22 (link)). Knockdown efficiency was evaluated via densitometry in Western blotting using Bio-Rad ImageLab version 4.1 software on a Chemi-Doc gel imaging system (Bio-Rad), normalizing Rab2a expression to that of β-actin for each sample.
Retroviral supernatants were generated in HEK 293T cells transfected for 48 h h with pCLXSN derivatives and the ecotropic helper plasmid pCL-Eco (Retromax, Imgenex), as described previously (22 (link)). Retroviral supernatants were added to BMMs (2:5 [vol/vol] ratio) for 48 to 60 h prior to paraformaldehyde (PFA) fixation and 24 to 36 h prior to infection.
Smith E.P., Cotto-Rosario A., Borghesan E., Held K., Miller C.N, & Celli J. (2020). Epistatic Interplay between Type IV Secretion Effectors Engages the Small GTPase Rab2 in the Brucella Intracellular Cycle. mBio, 11(2), e03350-19.
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2

Nrf2 Knockdown in NHEM Cells

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NHEM were transfected with siNC and siNrf2 siRNA (Dharmacon) using Amaxa nucleofector according to the manufacturer’s protocol.
Sample A., Zhao B., Wu C., Qian S., Shi X., Aplin A, & He Y.Y. (2017). The Autophagy Receptor Adaptor p62 is Up-regulated by UVA Radiation in Melanocytes and in Melanoma Cells†. Photochemistry and photobiology, 94(3), 432-437.
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3

Nub1 and Atg7 Knockdown in EL4/NC Cells

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One million EL4/NC NEDD8-GFP cells were harvested and transfected with 1 μM mouse Nub1 or Atg7 siRNA (Dharmacon, Lafayette, CO, USA), or Scrambled siRNA (as a negative control) using the Amaxa Nucleofector as described above. Transfected cells were incubated in growth media for 48 h before being processed for Western blot analysis.
Vijayasimha K., Tran M.V., Leestemaker-Palmer A.L, & Dolan B.P. (2021). Direct Conjugation of NEDD8 to the N-Terminus of a Model Protein Can Induce Degradation. Cells, 10(4), 854.
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4

SIRT1 WT and KO MEF Cell Culture

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SIRT1 wild-type (WT) and knockout (KO) mouse embryonic fibroblast (MEF) cells (a gift from Dr. Xiaoling Li, NIEHS/NIH) were maintained in a monolayer culture in 95% air/5% CO2 at 37°C in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 units per mL penicillin, and 100 mg per mL streptomycin (Invitrogen, Carlsbad, California). MEF cells were cultured for fewer than 20 passages. Normal human epidermal keratinocytes (NHEK) were obtained from Clonetics (Lonza, Inc., Allendale, NJ) and cultured in KGM Gold BulletKit medium (Clonetics, Lonza) according to the manufacturer’s instructions. NHEK cells were cultured for fewer than 4 passages. Cells were transfected with negative control (NC) or siRNA (ON-TARGETplus SMARTpool, Dharmacon, Inc., Pittsburgh, PA) targeting SIRT1, using Amaxa Nucleofector according to the manufacturer’s instructions as described previously (22 (link), 23 ).
Ming M., Zhao B., Shea C.R., Shah P., Qiang L., White S.R., Sims D.M, & He Y.Y. (2014). Loss of sirtuin 1 (SIRT1) disrupts skin barrier integrity and sensitizes mice to epicutaneous allergen challenge. The Journal of allergy and clinical immunology, 135(4), 936-945.e4.
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