Nanopure water

Manufactured by Merck Group

Sourced in United States, Germany

Nanopure water is a high-purity laboratory-grade water purification system. It produces ultrapure water by using a combination of filtration and deionization technologies. The core function of Nanopure water is to provide consistently pure water for various laboratory applications, such as analytical instrumentation, cell culture, and other research purposes.

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Lab products found in correlation

28 protocols using nanopure water

Trace Metal Analysis of Environmental Samples

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Following proper protocols (wearing gloves, sample labeling, etc.), participants collected water, soil, settled dust, and plant samples. Participants collected one 50ml water sample in a trace metal-free 50-mL tube (VWR, Cat. Number 89049–17) after running the water for three minutes prior to sample collection. In addition, the participant collected a 50ml field blank sample near the water source also using trace metal-free 50-mL tubes. Nanopure water (>18 MΩ deionized Nanopure water, Millipore) was provided to participants as field blank water, which was carefully transferred into a clean and empty sample tube.
Upon arrival to the lab, samples were refrigerated. To measure dissolved metals, we followed the U.S Environmental Protection Agency (EPA) Method 200.8 (U.S. EPA, 1994a ). A 20g sub sample of each water sample was filtered using 40 um filters (Environmental Express), acidified with 0.2g of 2 Molar Nitric Acid (VWR Analytic) for shelf stability, and analyzed using Inductively Coupled Plasma Mass Spectroscopy (7700X, Agilent, Santa Clara CA).

Craiu A., Akopian T., Goldberg A, & Rock K.L. (1997). Two distinct proteolytic processes in the generation of a major histocompatibility complex class I-presented peptide. Proceedings of the National Academy of Sciences of the United States of America, 94(20), 10850-10855.

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Synthesis of Palladium Nanoparticles

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Polyvinylpyrrolidone (55 000 MW, PVP), sodium tetrachloropalladate 99.99% (Na₂PdCl₄), and potassium tetrachloropalladate ≥99.99% (K₂PdCl₄) were purchased from Aldrich. l-Ascorbic acid ≥99.0% (L-aa). Potassium chloride (KCl, ≥99.0%) was purchased from Sigma. Rhodium(iii) bromide hydrate (RhBr₃·xH₂O), and triethylene glycol (TREG), were acquired from Alfa-Aesar. Potassium bromide (KBr, 99+%) was obtained from Sigma-Aldrich. Nanopure water (18.2 MΩ cm) was used as well as ethylene glycol (anhydrous, 99.8%, EG) purchased from Sigma-Aldrich. Platinum acetylacetonate (Pt(acac)₂) was purchased from Aldrich and unaltered.

Gamler J.T., Leonardi A., Sang X., Koczkur K.M., Unocic R.R., Engel M, & Skrabalak S.E. (2020). Effect of lattice mismatch and shell thickness on strain in core@shell nanocrystals. Nanoscale Advances, 2(3), 1105-1114.

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Graphitized Carbon Chromatography for Serum Analysis

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Separations
were performed on graphitized carbon columns packed in house with
3 μm Hypercarb particles (Thermo Fisher, Madison, WI) using
a fused-silica capillary, 75 μm i.d., 360 μm o.d., and
75 cm long (Polymicro Technologies, Phoenix, AZ). Solvents A and B
consisted of 0.1% formic acid (Sigma-Aldrich, St. Louis, MO) in nanopure
water and 0.2% trifluoroacetic acid (Sigma-Aldrich) in acetonitrile
(Fisher Scientific, Pittsburgh, PA), respectively. The LC separation
was performed with a linear gradient from 30% to 60% solvent B provided
by an Agilent 1260 Series LC pump (Santa Clara, CA) over a period
of 100 min. Serum samples were loaded with 1% B solvent mixture, and
a 90 min gradient was used consisting of (time in minutes: % mobile
phase B) 0:1%, 1:4%, 2:6%, 91:30%, 95:95%, 96:95%, 100:1%.

Cox J.T., Kronewitter S.R., Shukla A.K., Moore R.J., Smith R.D, & Tang K. (2014). High Sensitivity Combined with Extended Structural Coverage of Labile Compounds via Nanoelectrospray Ionization at Subambient Pressures. Analytical Chemistry, 86(19), 9504-9511.

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Metabolomics and Proteomics Sample Preparation

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For metabolomics analyses, Optima^TM grade water, acetonitrile and formic acid were purchased from Fisher Scientific (Hampton, NH, United States). Authentic standards used for secondary metabolite metabolomics are described in Höhner et al. (2018) (link). For proteomics and RNA-Seq sample preparation, nanopure water was used and all chemicals were obtained from Sigma-Aldrich (St. Louis, MO, United States) and were of analytical grade unless otherwise noted.

Hixson K.K., Marques J.V., Wendler J.P., McDermott J.E., Weitz K.K., Clauss T.R., Monroe M.E., Moore R.J., Brown J., Lipton M.S., Bell C.J., Paša-Tolić L., Davin L.B, & Lewis N.G. (2021). New Insights Into Lignification via Network and Multi-Omics Analyses of Arogenate Dehydratase Knock-Out Mutants in Arabidopsis thaliana. Frontiers in Plant Science, 12, 664250.

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Optimized Electrophoretic Separation System

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The LE solution was prepared by adjusting 20 mmol/L KOH to pH 6.4 with the counterion, MES. The LE solution also contained 0.1% v/v Triton X-100 and 2% PVP. The TE solution consisted of 60 mmol/L glycine, 0.1% v/v Triton X-100, and 2% PVP and was titrated to pH 5.8 with MES. Both the LE and TE solutions contained PVP to suppress the EOF [31 (link)]. Triton X-100 was added to the electrolyte to facilitate sample movement. All electrolyte solutions were prepared using Nanopure water from Millipore.

Guo S., Jacroux T., Ivory C.F., Li L, & Dong W.J. (2019). Immunobinding-induced alteration in the electrophoretic mobility of proteins: An approach to studying the preconcentration of an acidic protein under cationic isotachophoresis. Electrophoresis, 40(9), 1314-1321.

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Synthesis of Electrochemical Nanocomposites

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Pluronic F-127 (F-127), bis(2-ethylhexyl)-sebacate (castor oil, or DOS 97.0%), tetrahydrofuran (THF, 99.9%), 2-aminobiphenyl (2-ABP, 97%), and potassium tetrakis[pentafluorophenyl] borate (KTFPB, 95%) were used as obtained from Sigma-Aldrich. Also, ammonium hexafluorophosphate, NH₄PF₆ (99.98%), ferrocenemethanol (FcMeOH, 97%), ammonium phosphate monobasic (98%) and ammonium phosphate dibasic (98%) were purchased from Sigma-Aldrich and used as obtained. Nanopure water (18.2 MΩ·cm, TOC 2 ppb; Milli-Q Integral 5 system, Millipore) was used for both the preparation of aqueous electrolyte solutions and NE synthesis.

Madawala H., Sabaragamuwe S.G., Elangovan S, & Kim J. (2020). In Situ Measuring Partition Coefficient at Intact Nanoemulsions: A New Application of Single Entity Electrochemistry. Analytical chemistry, 93(2), 1154-1160.

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Peptide Conjugation Using PEG Linker

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Ethyl acetate, n-hexane, tetrahydrofuran (THF), diethyl ether, N,N‐dimethylformamide (DMF, anhydrous), l-alanine, l-isoleucine, bis(trichloromethyl) carbonate, glycine, trichloromethyl chloroformate, Proteinase K, Trypsin and naproxen (Npx) were purchased from Sigma-Aldrich (USA). Monomethoxy-monoamino-terminated poly(ethylene glycol) (mPEG₄₅-NH₂, M_n = 2000 g/mol) was purchased from Rapp Polymere (Germany). All chemicals were used without further purification, unless otherwise noted. Nanopure water (18.2 MΩ•cm) was acquired from a Milli-Q water filtration system, Millipore Corp. (St. Charles, MO). Tris-HCl buffered saline and phosphate buffered saline (PBS) were purchased from Sigma-Aldrich (USA) as dry powders, and dissolved in deionized water (1.0 L) to obtain the desired buffer solutions.

Fan J., Li R., Wang H., He X., Nguyen T.P., Letteri R.A., Zou J, & Wooley K.L. (2017). Multi-responsive polypeptide hydrogels derived from N–carboxyanhydride terpolymerizations for delivery of nonsteroidal anti-inflammatory drugs (NSAIDs). Organic & biomolecular chemistry, 15(24), 5145-5154.

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Characterization of Plant Polyphenol Extract

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Morin, EA, naringin, quercetin, lead nitrate
(Pb(NO₃)₂), sodium chloride (NaCl), sodium hydroxide
(NaOH), and DPPH were purchased from Sigma (St. Louis, MO, USA). All
solvents were obtained from Merck (NJ, USA) unless otherwise stated.
All reagents were of analytical grade and used without further purification.
Nanopure water (resistivity ≥ 18 MΩ cm) from Milli-Q
system (Millipore GmbH, Germany) was used whenever required. In order
to prepare a mixture of flavonoids and polyphenols, we have used a
well-characterized (by using ultra-high-performance liquid chromatography
mass spectrometry; UHPLC–MS) extract from eight medicinal plants
(Table S1). We call the mixture to be SKP17LIV01.
The detailed characteristics and systems pharmacology of the extract
will be published elsewhere.

Adhikari A., Darbar S., Chatterjee T., Das M., Polley N., Bhattacharyya M., Bhattacharya S., Pal D, & Pal S.K. (2018). Spectroscopic Studies on Dual Role of Natural Flavonoids in Detoxification of Lead Poisoning: Bench-to-Bedside Preclinical Trial. ACS Omega, 3(11), 15975-15987.

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Fabrication of Graphene-Based Plasmonic Nanostructures

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Monolayer graphene on copper foil was purchased from Grolltex (SC6 × 6). Polystyrene sheets were purchased from Scientific Explorer-Poof Slinky (item number 0SD300-10A). TFA gold etchant was purchased from Transene Company, Inc. Polydimethylsiloxane (Sylgard 184), hydrogen tetrachloroaurate (HAuCl₄*3H₂O) (> 99.9%), poly(allylamine hydrochloride) (molecular weight 50,000), isopropanol (ACS Plus grade), and 200 proof ethanol (HPLC grade) were purchased from Sigma-Aldrich. Sodium citrate dihydrate (lab grade) was purchased from Fischer Scientific. Methoxy-poly(ethylene glycol)-thiol (mPEG-SH, 5 kDa, 20 kDa, and 40 kDa) was purchased from Creative PEGWorks. Nanopure water (18 MΩ⋅m) was obtained from a Millipore system. All chemicals were used as received without further purification.

Gole M.T., Yin Z., Wang M.C., Lin W., Zhou Z., Leem J., Takekuma S., Murphy C.J, & Nam S. (2021). Large scale self-assembly of plasmonic nanoparticles on deformed graphene templates. Scientific Reports, 11, 12232.

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Synthesis and Characterization of Ferrocene-Based Electrolytes

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Pluronic F-127 (F-127), bis(2-ethylhexyl)- sebacate (DOS, 97.0%), tetrahydrofuran (THF, 99.9%), Ferrocene Methanol (FcMeOH, 97.0%), potassium tetrakis (pentafluorophenyl) borate (KTHPB, 97.0%), ammonium hexafluorophosphate (NH₄PF₆, 99.98%), ammonium chloride (NH₄Cl, 99.5%) and potassium nitrate (KNO₃, 99.0%) were purchased from Sigma-Aldrich used as obtained. Uranyless negative stain, and glass vials were purchased from Fisher Scientifics. Nanopure water (18.2 MΩ·cm, TOC 2 ppb; Milli-Q Integral 5 system, Millipore) was used to prepare all the aqueous electrolyte solutions as well as in NE synthesis.

Sabaragamuwe S.G., Madawala H., Puri S.R, & Kim J. (2020). Towards Ultralow Detection Limits of Aromatic Toxicants in Water using Pluronic Nanoemulsions and Single-Entity Electrochemistry. Analytica chimica acta, 1139, 129-137.

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