Bisindolylmaleimide 1 13298

Manufactured by Cayman Chemical

Sourced in United States

Bisindolylmaleimide I (13298) is a laboratory product manufactured by Cayman Chemical. It is a small molecule compound used as a research tool in biochemical and cell-based studies. The core function of Bisindolylmaleimide I is to inhibit protein kinase C (PKC) and other related kinases. This product is intended for laboratory research use only.

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Lab products found in correlation

Doxorubicin, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Azd7762 s1532, by Selleck Chemicals (1 mentions) Rpmi culture medium, by Thermo Fisher Scientific (1 mentions) Mcf 7 breast carcinoma cells, by American Type Culture Collection (1 mentions) Actin 4967, by Cell Signaling Technology (1 mentions) Superscript reverse transcriptase, by Thermo Fisher Scientific (1 mentions) Phosphatidylserine, by Avanti Polar Lipids (1 mentions) Actinomycin d, by Merck Group (1 mentions) Porcine brain sphingomyelin, by Avanti Polar Lipids (1 mentions) Protease and phosphatase inhibitor, by Selleck Chemicals (1 mentions) Ripa cell lysis buffer, by Cell Signaling Technology (1 mentions) Histamine, by Merck Group (1 mentions) Anti pcreb 9198s, by Cell Signaling Technology (1 mentions) H 89 dihydrochloride hydrate, by Merck Group (1 mentions) Irdye 680rd, by LI COR (1 mentions) Indomethacin, by Merck Group (1 mentions)

2 protocols using bisindolylmaleimide 1 13298

Mechanistic Study of DNA Damage Response

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MCF7 breast carcinoma cells were obtained from ATCC (Manassas, VA, USA). RPMI culture medium, fetal bovine serum and SuperScript reverse transcriptase were obtained from Invitrogen (Carisbad, CA, USA). Antibodies for nSMase2 (H195), Sp1 (PEP2) and Sp3 (D20) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies for Chk1 (2360), Chk2 (2662), ATM (2873), ATR (2790), p53 (9282) and actin (4967) were from Cell Signaling Technologies (Beverly, MA, USA). The inhibitors AZD7762 (S1532) and MK-8776 (S2735) were from Selleck Chem (Boston, MA, USA). Go6976 (2253) was purchased from Tocris Biosciences (Bristol, UK). Bisindolylmaleimide I (13298) was obtained from Cayman Chemicals (Ann Arbor, MI, USA). siRNA for TP53 (s607) and CREB3L1 (s40546) were from Life Technologies (Grand Island, NY, USA). All other siRNAs, CHEK1 (SI00024570), CHEK2 (SI00095305), ATM (SI00000840), ATR (SI00023107), Sp1 (SI150983) and Sp3 (SI0004788) were from Qiagen (Hiden, Germany). Porcine brain sphingomyelin and phosphatidylserine were from Avanti Polar Lipids (Alabaster, AL, USA). Doxorubicin, actinomycin D and, unless indicated otherwise, all other chemicals were obtained from Sigma (St. Louis, MO, USA).

Shamseddine A.A., Clarke C.J., Carroll B., Airola M.V., Mohammed S., Rella A., Obeid L.M, & Hannun Y.A. (2015). P53-dependent upregulation of neutral sphingomyelinase-2: role in doxorubicin-induced growth arrest. Cell Death & Disease, 6(10), e1947-.

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Phosphorylation signaling in vascular smooth muscle cells

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The primary antibody against total VASP (610448) was from BD biosciences (Franklin Lakes, NJ, USA). Anti-pCREB (9198S) and RIPA cell lysis buffer (9806) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-pHsp20 (58522) and anti-β-actin (58522) were purchased from Abcam (Cambridge MA, USA) and Sigma (St. Louis, MO, USA) respectively. Secondary antibodies IRDye 680RD or 800CW were from LI-COR (Lincoln, NE, USA). DGK inhibitor I (R59022), 3-Isobutyl-1-methylxanthine (IBMX), forskolin, histamine, H-89 dihydrochloride hydrate, indomethacin, and all other standard reagents were obtained from Sigma Life Sciences (St. Louis, MO, USA) unless otherwise mentioned. Protease and phosphatase inhibitors were from Bimake (Houston, TX, USA). Bisindolylmaleimide I (13298) was purchased from Cayman Chemicals (Ann Arbor, MI, USA). U0126 (S1102) was procured from Selleckchem (Houston, TX, USA). All polyacrylamide gel casting, running, and transfer reagents and equipment were from Bio-Rad Laboratories (Hercules, CA, USA) or from previously identified sources (Michael et al., 2019 (link); Sharma et al., 2016 (link)).

Yadav S.K., Sharma P., Shah S.D., Panettieri RA J.r., Kambayashi T., Penn R.B, & Deshpande D.A. (2021). Autocrine regulation of airway smooth muscle contraction by diacylglycerol kinase. Journal of cellular physiology, 237(1), 603-616.

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