Dulbecco s modified eagle s medium ham s f12

Manufactured by Merck Group

Dulbecco's modified Eagle's medium/Ham's F12 is a cell culture medium formulation commonly used in the cultivation of a variety of cell types. It is a mixture of nutrients, vitamins, and other components designed to support cell growth and maintenance in a laboratory setting.

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Lab products found in correlation

Insulin, by Merck Group (2 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions) Collagenase type 4, by Merck Group (1 mentions) Matrigel, by BD (1 mentions) Mycozap plus cl, by Lonza (1 mentions) Hs578t, by American Type Culture Collection (1 mentions) Facsvantage cell sorter, by BD (1 mentions) Mda mb 231, by American Type Culture Collection (1 mentions) Hydrocortisone, by Merck Group (1 mentions) Recombinant human epidermal growth factor egf, by Merck Group (1 mentions) Epilife defined growth supplement, by Thermo Fisher Scientific (1 mentions) Epilife medium, by Thermo Fisher Scientific (1 mentions) Non essential amino acids (neaa), by Lonza (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Cytiva (1 mentions) Sodium pyruvate, by Lonza (1 mentions) Oral keratinocyte medium, by ScienCell (1 mentions) Oral keratinocyte growth supplement, by ScienCell (1 mentions) Penicillin streptomycin solution, by ScienCell (1 mentions)

Close spelling variants of this product

Dulbecco's modified Eagle's medium/Ham's F12 medium Dulbecco’s Modified Eagle’s Medium and Ham F-12 Nutrient Mix (DMEM/F12), Dulbecco's modified Eagle's medium (DMEM)/Ham's F12 Ham F12 medium Dulbecco’s modified F12 medium

5 protocols using dulbecco s modified eagle s medium ham s f12

Culturing Colorectal Cell Lines

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CRC cell lines (SW620 and SW1417) and a normal human colorectal cell line (FHC; cat. no. CRL-1831), were acquired from the Cell Bank of the Chinese Academy of Medical Sciences (Beijing, China). SW620, SW1417 and FHC cells were cultured at 37°C and 5% CO₂ in Dulbecco's modified Eagle's medium/Ham's F-12 (Sigma-Aldrich; Merck KGaA), supplemented with 10% fetal bovine serum (FBS; cat. no. 10099141; Gibco; Thermo Fisher Scientific, Inc.), antibiotic-antimycotic (1:100, cat. no. 15240096; Thermo Fisher Scientific, Inc.)

Li Y, & Chen Z. (2020). The oncogenic role of microRNA-500a in colorectal cancer. Oncology Letters, 19(3), 1799-1805.

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Isolation and Culture of FDB Muscle Fibers

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The FDB muscles were incubated for 38 min at 37°C in an oxygenated “Krebs-Hepes” solution [135.5 mM NaCl, 1.2 mM MgCl₂, 5.9 mM KCl, 11.5 mM glucose, 11.5 mM Hepes, and 1.8 mM CaCl₂ (pH 7.3) containing 0.2% collagenase type IV (Sigma-Aldrich, St. Louis, MO, USA). Muscles were then washed twice in Dulbecco’s modified Eagle’s medium/Ham’s F12 (Sigma-Aldrich) supplemented with 2% fetal bovine serum (FBS) (Sigma-Aldrich) and mechanically dissociated by repeated passages through fire-polished Pasteur pipettes of progressively decreasing diameter. Dissociated fibers were plated onto tissue culture dishes coated with Matrigel (BD Biosciences, San Jose, CA, USA) and allowed to adhere to the bottom of the dish for 2 hours. For Ca²⁺ measurements, cells were plated on glass-bottom MaTek disks. Culture dishes were kept in an incubator, with 5% CO₂ at 37°C for 2 hours to let the fibers attach (58 (link)).

Laurila P.P., Luan P., Wohlwend M., Zanou N., Crisol B., Imamura de Lima T., Goeminne L.J., Gallart-Ayala H., Shong M., Ivanisevic J., Place N, & Auwerx J. (2022). Inhibition of sphingolipid de novo synthesis counteracts muscular dystrophy. Science Advances, 8(4), eabh4423.

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Establishment of IBC and TNBC Cell Lines

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SUM149 cells (a kind gift from Dr. Steven Ethier, MUSC) were used as a model of human IBC. Cells were maintained in 2D in Dulbecco's Modified Eagles Medium/Ham's F-12 (Sigma-Aldrich) supplemented with 5 % FBS (Hyclone), 5 μg/ml insulin (Sigma-Aldrich), 1 μg/ml hydrocortisone (Sigma-Aldrich), and 1 % Mycozap Plus-CL (Lonza). The invasive human TNBC breast cancer cell lines, MDA-MB-231 and Hs578T, were purchased from ATCC. MDA-MB-231 cells were maintained in medium composed of DMEM supplemented with 10 % FBS, 4 mM l-glutamine, and 1 % Mycozap Plus-CL. Hs578T cells were cultured and maintained in DMEM medium supplemented with 10 % FBS, 5 μg/ml insulin, 4 mM l-glutamine, and 1 % Mycozap Plus-CL. All cell lines were maintained in T-25 flasks in a humidified incubator (5 % CO₂) at 37 °C.
For some experiments, the SUM149 cells were transduced to express red fluorescent protein (RFP). Briefly, 50,000 cells were seeded in a 6-well dish and transduced with 20 μl cignal RFP viral particles (Qiagen). Transduced cells were flow sorted using the BD FACS vantage cell sorter to select the population of cells that expressed RFP. The SUM149-RFP cell line was maintained in 2D as described above for the wild-type cells.

Aggarwal N., Santiago A.M., Kessel D, & Sloane B.F. (2015). Photodynamic therapy as an effective therapeutic approach in MAME models of inflammatory breast cancer. Breast cancer research and treatment, 154(2), 251-262.

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Oral Keratinocyte Culture Protocol

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EpiLife medium, EpiLife defined growth supplement (EDGS), and trypsin-EDTA were purchased from Life Technologies (Grand Island, NY). Oral keratinocyte medium, oral keratinocyte growth supplement, and penicillin/streptomycin solution (P/S) were obtained from ScienCell Research Laboratory (Carlsbad, CA). Dulbecco’s modified Eagle’s medium/Ham’s F12, insulin, cholera toxin from vibro cholera, and human recombinant epidermal growth factor (EGF) were delivered by Sigma-Aldrich (St. Louis, MO). Roswell Park Memorial Institute medium 1640, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), L-glutamine, non-essential amino acids (NEAA), and sodium pyruvate were obtained from Lonza (Walkersville, MD). Fetal bovine serum (FBS) was purchased from Hyclone Laboratories (Logan, UT). All cell culture and plastic wares were purchased from Thermo Fisher Scientific (Waltham, MA).

Islam R., Eidet J.R., Badian R.A., Lippestad M., Messelt E., Griffith M., Dartt D.A, & Utheim T.P. (2017). Tissue Harvesting Site and Culture Medium Affect Attachment, Growth, and Phenotype of Ex Vivo Expanded Oral Mucosal Epithelial Cells. Scientific Reports, 7, 674.

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Perfusion and Sectioning of Rat Brainstem

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One week after CTB injections, all rats were perfused with 500 mL tissue culture medium (Dulbecco's Modified Eagle's Medium/ Ham's F12, Catalogue #D-8900; Sigma, St. Louis, MO) followed by 1L of fixative (4% formaldehyde in 0.1M phosphate buffer, pH 7.4). Brains and spinal cords were removed and post-fixed for 3-4 days. Spinal cords were washed and stored in 0.1M phosphate buffer with 0.05% sodium azide at 4°C until injection sites were located. Brains were washed in 0.1M phosphate buffer, pH 7.4, and briefly stored at 4 °C until blocking and sectioning. Brainstems from pairs of sedentary and active rats were given different marks and then blocked from the spinomedullary junction to the cerebromedullary interface. Pairs of blocks were individually embedded in agarose gel and transversely sectioned at 150 μm on a Vibratome (Leica Microsystems, Buffalo Grove, IL) into Corning Netwells® (Fisher Scientific, Pittsburgh, PA) so that each well contained brainstem sections from both rats. The brainstem of the additional rat, which was not part of either the active or sedentary group, was sectioned at 50 μm to provide better documentation of the distribution of bulbospinal catecholaminergic RVLM neurons. Sections were stored in 0.1M phosphate buffer with 0.05% sodium azide at 4°C until immunohistochemical processing.

Mischel N.A., Llewellyn-Smith I.J, & Mueller P.J. (2014). Physical (in)activity dependent structural plasticity in bulbospinal catecholaminergic neurons of rat rostral ventrolateral medulla. The Journal of comparative neurology, 522(3), 499-513.

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