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Lipopolysaccharide from rhodobacter sphaeroides lps rs catalog tlrl rslps

Manufactured by InvivoGen

Lipopolysaccharide from Rhodobacter sphaeroides (LPS-RS) is a purified bacterial component. It is a Toll-like receptor 4 (TLR4) antagonist.

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2 protocols using lipopolysaccharide from rhodobacter sphaeroides lps rs catalog tlrl rslps

1

Inhibitor Compounds for Inflammation Studies

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TAK-242 (CAS 243984-11-4 Cat 6587) was purchased from R&D Systems (Minneapolis MN). Lipopolysaccharide from Rhodobacter sphaeroides (LPS-RS) (Catalog # tlrl-rslps) and MCC950 (CAS 210826-40-7, Catalog # inh-mcc) were purchased from InvivoGen (San Diego CA). Trans-ISRIB (CAS 1597403-47-8, Cat 5284) was from Tocris (Ellisville MO). Other chemicals, including glyphosate (CAS 1071-83-6), minocycline (CAS 13614-98-7, Cat# M2280000) and IL1-Ra (Cat# SRP 3084), quercetin (CAS 849061-97-8, PHR1488) and salts were obtained from Millipore Sigma Chemical Company. Roundup, a herbicide containing glyphosate, was purchased from a local store. Drugs were prepared as stock solutions in either ACSF or DMSO and diluted to final concentration at the time of experiment. The concentrations of TAK-242, LPS-RS and minocycline are based on our previous studies using those inhibitors against lipopolysaccharide (LPS) and acrylamide21 (link),22 (link). The concentrations of MCC950 were also based on our previous paper22 (link). The dose of TAK-242 in the behavioral study followed a proceeding report by Ono et al.30 (link).
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2

TLR4 Antagonist-Mediated Neuroprotection

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TAK-242 (CAS 243984-11-4 Cat 6587) was purchased from R&D Systems (Minneapolis MN). Lipopolysaccharide from Rhodobacter sphaeroides (LPS-RS) (Catalog # tlrl-rslps) and MCC950 (CAS 210826-40-7, Catalog # inh-mcc) were purchased from InvivoGen (San Diego CA). Trans-ISRIB (CAS 1597403-47-8, Cat 5284) was from Tocris (Ellisville MO). Other chemicals, including glyphosate (CAS 1071-83-6), minocycline (CAS 13614-98-7, Cat# M2280000) and IL1-Ra (Cat# SRP 3084), quercetin (CAS 849061-97-8, PHR1488) and salts were obtained from Millipore Sigma Chemical Company. Roundup, a herbicide containing glyphosate, was purchased from a local store. Drugs were prepared as stock solutions in either ACSF or DMSO and diluted to final concentration at the time of experiment. The concentrations of TAK-242, LPS-RS and minocycline are based on our previous studies using those inhibitors against lipopolysaccharide (LPS) and acrylamide (Izumi, Cashikar, et al., 2021 (link); Izumi et al., 2022 (link)). The concentrations of MCC950 were also based on our previous paper (Izumi et al., 2022 (link)). The dose of TAK-242 in the behavioral study followed a proceeding report by Ono et al. (Ono et al., 2020 (link)).
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