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Murine b16 melanoma cell line

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The Murine B16 melanoma cell line is a widely used experimental model derived from a spontaneous melanoma tumor that occurred in a C57BL/6 mouse. This cell line is commonly utilized in cancer research to study melanoma biology and evaluate potential therapeutic interventions.

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Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Tramp c2, by American Type Culture Collection (2 mentions)

Spelling variants of this product

B16 cells (13 citations) B16 melanoma (9 citations) B16 cell line (6 citations) B16 melanoma cell line (4 citations) B16(F10) murine melanoma cell lines (2 citations)

3 protocols using murine b16 melanoma cell line

1

Murine Models for Lysosomal Acid Lipase

Cited 1 time
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lal+/+ and lal−/− mice of the FVBN background were bred in house. c-fms-rtTA/(TetO)7-CMV-hLAL; lal−/−(Tg/KO) triple mice of the FVBN background is a previously generated triple transgenic mouse model with myeloid-specific doxycycline-inducible expression of wild-type human LAL (hLAL) in lal−/− mice under the control of the c-fms promoter 3 (link), 7 (link). All scientific protocols involving the use of animals have been approved by the Institutional Animal Care and Use Committee of Indiana University School of Medicine and followed guidelines established by the Panel on Euthanasia of the American Veterinary Medical Association. Animals were housed under Institutional Animal Care and Use Committee-approved conditions in a secured animal facility at Indiana University School of Medicine.
The murine B16 melanoma cell line, Lewis lung carcinoma (LLC) cell line, and transgenic mouse prostate cancer (TRAMP-C2) cell line (purchased from ATCC) were cultured in DMEM supplemented with 10% FBS (Gibco).
Zhao T., Du H., Ding X., Walls K, & Yan C. (2014). Activation of mTOR Pathway in Myeloid-derived Suppressor Cells Stimulates Cancer Cell Proliferation and Metastasis in lal−/− Mice. Oncogene, 34(15), 1938-1948.
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2

Murine Models for Lysosomal Acid Lipase

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
lal+/+ and lal−/− mice of the FVBN background were bred in house. c-fms-rtTA/(TetO)7-CMV-hLAL; lal−/−(Tg/KO) triple mice of the FVBN background is a previously generated triple transgenic mouse model with myeloid-specific doxycycline-inducible expression of wild-type human LAL (hLAL) in lal−/− mice under the control of the c-fms promoter 3 (link), 7 (link). All scientific protocols involving the use of animals have been approved by the Institutional Animal Care and Use Committee of Indiana University School of Medicine and followed guidelines established by the Panel on Euthanasia of the American Veterinary Medical Association. Animals were housed under Institutional Animal Care and Use Committee-approved conditions in a secured animal facility at Indiana University School of Medicine.
The murine B16 melanoma cell line, Lewis lung carcinoma (LLC) cell line, and transgenic mouse prostate cancer (TRAMP-C2) cell line (purchased from ATCC) were cultured in DMEM supplemented with 10% FBS (Gibco).
Zhao T., Du H., Ding X., Walls K, & Yan C. (2014). Activation of mTOR Pathway in Myeloid-derived Suppressor Cells Stimulates Cancer Cell Proliferation and Metastasis in lal−/− Mice. Oncogene, 34(15), 1938-1948.
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3

In vivo Mouse Tumor Modeling

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Wild-type (lal+/+) and lal−/− mice of the FVB/N background were bred in house. c-fms-rtTA/(TetO)7-CMV-dnPPARγ bitransgenic mice of the FVB/N background is a previously generated bitransgenic mouse model [15 (link)]. All scientific protocols involving the use of animals have been approved by the Institutional Animal Care and Use Committee of Indiana University School of Medicine and followed guidelines established by the Panel on Euthanasia of the American Veterinary Medical Association. Animals were housed under Institutional Animal Care and Use Committee-approved conditions in a secured animal facility at Indiana University School of Medicine.
The murine B16 melanoma cell line, Lewis lung carcinoma (LLC) cell line, and murine endothelial cell (SVEC) line (purchased from ATCC, Manassas, VA, USA) were cultured in DMEM supplemented with 10% FBS (Gibco, Grand Island, NY, USA).
Zhao T., Du H., Blum J.S, & Yan C. (2015). Critical role of PPARγ in myeloid-derived suppressor cell-stimulated cancer cell proliferation and metastasis. Oncotarget, 7(2), 1529-1543.
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