RPPA was performed as previously published by us [16 (link)] (more details in the Supplementary Data ). The following antibodies were used: Parp-1#sc-7150 (Santa Cruz, TX, USA). Cleaved caspase-9 (Asp315) #9505, cleaved caspase-9 (Asp330) #9501, cleaved caspase-7 (Asp198) #9491 and XIAP#2042 (Cell Signaling, MA, USA).
Cleaved caspase 7 asp198
Manufactured by Cell Signaling Technology
Sourced in United States
Cleaved caspase-7 (Asp198) is a lab equipment product that detects the presence of the cleaved form of caspase-7 protein. Caspase-7 is a member of the cysteine-aspartic acid protease (caspase) family, which play essential roles in programmed cell death (apoptosis) and inflammation.
Automatically generated - may contain errors
Lab products found in correlation
Xiap 2042, by Cell Signaling Technology (1 mentions)
Parp 1 sc 7150, by Santa Cruz Biotechnology (1 mentions)
Cleaved caspase 9 asp330, by Cell Signaling Technology (1 mentions)
Anti mouse igg hrp, by Agilent Technologies (1 mentions)
Cleaved parp asp214, by Cell Signaling Technology (1 mentions)
Anti rabbit igg hrp, by Agilent Technologies (1 mentions)
Cyclin d1, by Cell Signaling Technology (1 mentions)
Cyclin d3, by Cell Signaling Technology (1 mentions)
C myc, by Cell Signaling Technology (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
Cleaved caspase 3 asp175, by Cell Signaling Technology (1 mentions)
Yap1 ab52771 antibody, by Abcam (1 mentions)
P rb ser807 811, by Cell Signaling Technology (1 mentions)
2 protocols using cleaved caspase 7 asp198
1
RPPA Analysis of Apoptosis Markers
2
Western Blot Analysis Specifications
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Western blot analysis was performed in our previous study.5 (link) Some of the western blot results were quantified by densitometric scans using ImageJ (version 1.48p; W Rasband, National Institutes of Health; available at http://rsb.info.nih.gov/ij/ ) for the following Pearson’s correlation analysis. The primary antibodies of TEAD1 (sc-376113), TEAD4 (sc-134071), CTGF (L-20) (sc-14939) and Cyr-61 (sc-374129) were commercially available from Santa Cruz (Dallas, TX, USA). YAP1 (ab52771) antibody was achieved from Abcam (Cambridge, MA, USA). TEAD2 (SAB2102402) and TEAD3 (AV38278) antibodies were obtained from Sigma-Aldrich (St Louis, MO, USA). Other primary antibodies were from Cell Signaling (Danvers, MA, USA), including p21 (#2946), p27 (#2552), p-Rb (Ser807/811) (#9308), cleaved-caspase 3 (Asp175) (#9661), cleaved-caspase 7 (Asp198) (#9491), cleaved-PARP (Asp214) (#9541), cyclin D1 (#2978), cyclin D3 (#2936), CDK4 (#12790), CDK6 (#3136), c-Myc (#9402) and GAPDH (#2118). Anti-Mouse IgG-HRP (Dako, Glostrup, Denmark, 00049039, 1:30 000) and anti-Rabbit IgG-HRP (Dako, 00028856, 1:10 000) were used for secondary antibodies.
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