The human lung SQCC cell lines, H520 was obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA), and HCC95, H1703, and SK-MES-1 were obtained from the Korean Cell Line Bank (Seoul, Korea), and cultured in RPMI 1640 medium (H520, HCC95, H1703) and Dulbecco’s modified Eagle’s medium (SK-MES-1) supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin–streptomycin (Hyclone, Logan, UT, USA). The HBEpC cells were obtained from PromoCell GmbH (Heidelberg, Germany) and cultured in an airway epithelial cell growth medium containing 2.46% SupplementMix (PromoCell GmbH). Cell culture was performed as previously described [16 (link)]. Each cell line was cultured in four biological replicates.
H1703
Manufactured by Korean Cell Line Bank
Sourced in United States
H1703 is a cell culture incubator designed for use in laboratory settings. It provides a controlled environment for the growth and maintenance of cell lines. The incubator maintains a stable temperature, humidity, and CO2 concentration to support optimal cell culture conditions.
Automatically generated - may contain errors
Lab products found in correlation
H1299, by Korean Cell Line Bank (3 mentions)
Sk mes 1, by Korean Cell Line Bank (1 mentions)
Hcc95, by Korean Cell Line Bank (1 mentions)
Supplementmix, by PromoCell (1 mentions)
Bm cyclin, by Roche (1 mentions)
H1792, by Korean Cell Line Bank (1 mentions)
H1975, by Korean Cell Line Bank (1 mentions)
Hcc2108, by Korean Cell Line Bank (1 mentions)
Sk lu 1, by Korean Cell Line Bank (1 mentions)
Lly 507, by MedChemExpress (1 mentions)
Hek293t cells, by Korean Cell Line Bank (1 mentions)
Orotate, by Merck Group (1 mentions)
Uridine, by Thermo Fisher Scientific (1 mentions)
Docetaxel, by Merck Group (1 mentions)
Penicillin streptomycin solution, by Welgene (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Rpmi 1640, by Merck Group (1 mentions)
Rpmi 1640, by Welgene (1 mentions)
5 protocols using h1703
1
Lung Squamous Cell Carcinoma Cell Lines
2
Cell Culture of Human Lung ADC
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Human lung ADC cell lines were obtained from the American Type Culture Collection (H1792) or the Korean Cell Line Bank (H23, H358, H1299, H1703, H1792, H1975, HCC1171, HCC2108, and SK-LU-1). All cells were grown at 37°C with 5% CO2 in RPMI-1640 or Dulbecco's modified Eagle's medium (HyClone) containing 10% fetal bovine serum (HyClone) and 1% penicillin/streptomycin (HyClone). All cells were monitored for Mycoplasma contamination using a PCR-based method for detection. Detected Mycoplasma were eliminated using BM cyclins (Roche).
3
Establishing Luciferase-Expressing Lung Cancer Cell Lines
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The human lung cancer cell lines H1299 and H1703 were purchased from the Korean Cell Line Bank (Seoul, South Korea) and cultured in RPMI supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin in a humidified atmosphere with 5% CO2 at 37 °C. LLY507 (HY-19313) was purchased from MedChemExpress. To establish luciferase-expressing H1299 cells (shCont and shSMYD2), H1299 cells were infected with F-luc lentivirus (Capital Biosciences, VSL-0044), and the cells were selected with puromycin (2 μg/ml) for 2 weeks.
4
Culturing Diverse Lung Cell Lines
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L132 (non‐transformed lung epithelial cell), A549, H157, H1299, H1703 (lung cancer cell lines) and HEK293T cells were purchased from Korean Cell Line Bank (Seoul, Korea). All lung cancer cell lines and L132 cells were maintained in RPMI‐1640 medium supplemented with 10% FBS and 1% penicillin‐streptomycin (Hyclone, Logan, UT, USA). HEK293T cells were maintained in a DMEM medium supplemented with 10% FBS and 1% penicillin‐streptomycin in a humidified chamber with 5% CO2 at 37 °C.
5
Lung Cancer Cell Culture Protocol
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Human lung cancer cells (H460, H1703, A549, and H358) were purchased from the Korean Cell Line Bank (Seoul, Korea). H1703 cells were cultured in RPMI-1640 (WelGENE, Gyeongsan, Korea) supplemented with 10% FBS (JR Scientific, CA, USA), 1% penicillin/streptomycin solution (WelGENE, Korea), 4500 mg/L d -glucose, 2 mM l -glutamine, and 10 mM HEPES. The other cancer cells were maintained in RPMI-1640 medium containing 10% Fetal Bovine Serum (FBS), 1% antibiotics, and 2.05 mM l -glutamine (Additional files 1 , 2 ).
Docetaxel and orotate (Sigma-Aldrich Inc., MO, USA) were dissolved in DMSO. UMP (Sigma, USA) and uridine (Thermo Fisher Scientific, USA) were dissolved in D.W. SH003 was provided by Hanpoong Pharm and Foods Company (Jeonju, Republic of Korea). The extraction method for SH003 was as previously described [15 (link)].
Docetaxel and orotate (Sigma-Aldrich Inc., MO, USA) were dissolved in DMSO. UMP (Sigma, USA) and uridine (Thermo Fisher Scientific, USA) were dissolved in D.W. SH003 was provided by Hanpoong Pharm and Foods Company (Jeonju, Republic of Korea). The extraction method for SH003 was as previously described [15 (link)].
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