Easy nlc1200 nano

Manufactured by Thermo Fisher Scientific

The Easy nLC1200 nano is a liquid chromatography system designed for nanoscale separations. It features a compact design and is capable of delivering precise and reproducible flow rates for high-performance liquid chromatography (HPLC) applications.

Automatically generated - may contain errors

Lab products found in correlation

Acclaim pepmap rslc analytical column, by Thermo Fisher Scientific (1 mentions) Acclaim pepmap 100 trap column, by Thermo Fisher Scientific (1 mentions) Q exactive plus, by Thermo Fisher Scientific (1 mentions) Q exactive hf x quadrupole orbitrap mass spectrometer, by Thermo Fisher Scientific (1 mentions) Reprosil pur 120 c18 aq 2.4 μm beads, by Dr. Maisch (1 mentions)

Close spelling variants of this product

EASY-nLC1200 nano-UPLC Liquid Phase System (2 citations)

2 protocols using easy nlc1200 nano

LC–MS/MS Analysis of Peptides

Check if the same lab product or an alternative is used in the 5 most similar protocols

LC–MS/MS analysis was carried out using the Q Exactive Plus mass spectrometer (Thermo Fisher Scientific) coupled to the Easy nLC1200 nano-flow UPLC. Peptides (500 ng of each sample) were injected into the Acclaim PepMap 100 trap column (nanoViper C18, 100 μm × 2 cm, Thermo Scientific) and separated by the Acclaim Pep Map RSLC analytical column (nanoViper C18, 50 μm × 15 cm, Thermo Scientific) set at the flow rate of 300 nL/min. The solvent gradients were set as a linear gradient of 8~38% mobile phase B (80% acetonitrile contains 0.1% formic acid) over 102 min through 120 min run time. The sample was atomized using the nanoESI source. The data-dependent acquisition (Top 20) was carried out using MS survey scans in the 350~1700 m/z range with 70,000 mass resolution. For subsequent MS/MS analysis, the resolution was set to 17,500, and the isolation window was set to 1.6 m/z. The normalized collision energy was 27 eV. Peptides with charge 2~7 were selected.

Liu S., Liu T., Wang E., Cheng Y., Liu T., Chen G., Guo M, & Song B. (2022). Dissecting the Chloroplast Proteome of the Potato (Solanum Tuberosum L.) and Its Comparison with the Tuber Amyloplast Proteome. Plants, 11(15), 1915.

+ Open protocol

+ Expand

Nano-UHPLC-MS/MS Peptide Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peptides were resuspended in water with 0.1 % formic acid (FA) and analyzed using EASY-nLC 1200 nano-UHPLC coupled to a Q Exactive HF-X Quadrupole-Orbitrap mass spectrometer (Thermo Scientific). The chromatography column consisted of a 50 cm long, 75 μm i.d. microcapillary capped by a 5 μm tip and packed with ReproSil-Pur 120 C18-AQ 2.4 μm beads (Dr. Maisch GmbH). LC solvents were 0.1 % FA in H₂O (Buffer A) and 0.1 % FA in 90 % MeCN: 10 % H₂O (Buffer B). Peptides were eluted into the MS at a flow rate of 300 nL/min. over a 240 min. linear gradient (5-35 % Buffer B) at 65 °C. Data was acquired in data-dependent mode (top-20, NCE 28, R = 7,500) after full MS scan (R = 60,000, m/z 400-1,300). Dynamic exclusion was set to 10 s, peptide match to prefer and isotope exclusion was enabled.

Amatuni A., Shuster A., Adibekian A, & Renata H. (2020). Concise Chemoenzymatic Total Synthesis and Identification of Cellular Targets of Cepafungin I. Cell chemical biology, 27(10), 1318-1326.e18.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!