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20xuas ivs gcamp6s

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The 20XUAS-IVS-GCaMP6s is a genetic construct that can be used to express the GCaMP6s calcium indicator in Drosophila melanogaster. GCaMP6s is a genetically encoded fluorescent calcium indicator that can be used to monitor changes in intracellular calcium levels. The 20XUAS-IVS sequence provides a high level of gene expression when the construct is driven by a GAL4 transcription factor.

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Lab products found in correlation

Uas trpa1, by Bloomington Drosophila Stock Center (2 mentions) Th gal4, by Bloomington Drosophila Stock Center (2 mentions) Alrm lexa gad, by Bloomington Drosophila Stock Center (2 mentions) Uas wtrw gfp, by Bloomington Drosophila Stock Center (2 mentions) Lexaop r geco1, by Bloomington Drosophila Stock Center (2 mentions) Tdc2 gal4, by Bloomington Drosophila Stock Center (2 mentions) 13xlexaop2 ivs gcamp6s p10, by Bloomington Drosophila Stock Center (2 mentions) Uas kir2.1 egfp, by Bloomington Drosophila Stock Center (2 mentions) Uas oct tyrrrnai, by Bloomington Drosophila Stock Center (2 mentions) Uas shidn, by Bloomington Drosophila Stock Center (2 mentions) Uas cschrimson, by Bloomington Drosophila Stock Center (2 mentions) Adorkg03964ex, by Bloomington Drosophila Stock Center (2 mentions) Tdc2 lexa, by Bloomington Drosophila Stock Center (2 mentions) Th lexa, by Bloomington Drosophila Stock Center (2 mentions) Tsh gal8033, by Bloomington Drosophila Stock Center (2 mentions) Lexaop gcamp6s, by Bloomington Drosophila Stock Center (2 mentions) 10xuas ivs myr tdtomato, by Bloomington Drosophila Stock Center (2 mentions) Abdb gal4, by Bloomington Drosophila Stock Center (2 mentions) 20xuas ivs cschrimson mvenus, by Bloomington Drosophila Stock Center (2 mentions) R71g01 gal4, by Bloomington Drosophila Stock Center (2 mentions)

6 protocols using 20xuas ivs gcamp6s

1

Drosophila Neuronal Characterization Protocol

Cited 1 time
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Flies were cultured in standard cornmeal food at 25°C in 12h/12h light/dark cycles. Fly stocks include: w1118, alrm-Gal426 (link), alrm-LexA::GAD, UAS-wtrw-gfp, LexAop-R-GECO1 (Bloomington, 52224), tdc2-Gal4 (Bloomington, 9313), 20XUAS-IVS-GCaMP6s (Bloomington, 42746), 13XLexAop2-IVS-GCaMP6s-p10 (Bloomington, 44274), UAS-Kir2.1-EGFP (Bloomington, 6595), UAS-Oct-TyrRRNAi (Bloomington, 28332), th-Gal4 (Bloomington, 8848), UAS-wtrwVDRC (VDRC, 107423), Oct-TyrRhono (Kyoto, 109038), UAS-shiDN (Bloomington, 5822), UAS-TrpA1 (Bloomington, 26263), UAS-NpHR (Kyoto, 117008), UAS-CsChrimson (Bloomington, 55135), AdoRKG03964ex (Bloomington, 30868), tdc2-LexA::p6527 (link), UAS-wtrw2.2328 (link), wtrwex29 (link), tβhnM1830 , tdc2RO5431 , th-LexA::p6532 , tsh-Gal8033 .
Ma Z., Stork T., Bergles D.E, & Freeman M.R. (2016). Neuromodulators signal through astrocytes to alter neural circuit activity and behavior. Nature, 539(7629), 428-432.
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2

Drosophila Neurogenetics: Protocols and Stocks

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Flies were housed under standard conditions at 25 °C under a 12 hr light: 12 hr dark cycle. Fly stocks and sources: Drosophila melanogaster Canton-S, 20xUAS-IVS-GCaMP6s (Bloomington #42746, #42749), UAS-mCD8::GFP (#5130, #5137), LexAop-GCaMP6s (#53747), 10xUAS-IVS-myr::tdTomato (#32222), R71G01-Gal4 (#39599), AbdB-Gal4 (#55848) R25E04-Gal4 (#49125), and 20xUAS-IVS-CsChrimson.mVenus (#55134) were obtained from the Bloomington Stock Center. D. sechellia (14021-0248.25) and D. erecta (14021-0224.01) were obtained from the UCSD Stock Center. The following were gifts, obtained as indicated: D. ananassae and D. simulans (Richard Axel), D. simulans attP2039 36 (Yun Ding and David Stern, Janelia Research Campus); SplitP1-Gal4 24 (link) (David Anderson, Caltech); fruLexA37 (link) and fruGal416 (link) (Barry Dickson, HHMI/Janelia Farm Research Campus); D. melanogaster ppk23-Gal411 (link) (Kristin Scott, UC Berkeley); LexAop-myr::tdTomato (Cesar Mendes Columbia University); UAS-SPA-t2a-SPA-GFP 25 (link). Please see Supplemental Table 1 for detailed descriptions of genotypes used in each experiment.
Seeholzer L.F., Seppo M., Stern D.L, & Ruta V. (2018). Evolution of a central neural circuit underlies Drosophila mate preferences. Nature, 559(7715), 564-569.
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3

Genetic Manipulation of Drosophila Neurons

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Flies were kept at 25 °C on common cornmeal-yeast-sucrose food under light:dark cycles and 60–70% humidity. UAS-GFP-Aequorin strain was obtained from Martin et al.14 . Expression of GFP-apo-Aequorin was driven to KCs and DAs by 201y-gal4 and mb247-gal4, and TH-gal4 and R58E02, respectively. Either the driver and transgene lines were crossed to CSw− strain to generate larvae to use as genetic controls of the experiments. Also, mb247-gal4;UAS-tubP-Gal80ts 32 was crossed to either UAS-GFP-Aequorin strain or to control the specificity of the KC signal. Expression of 20XUAS-IVS-GCaMP6s (42749, Bloomington Stock Center) was driven to the KCs by 201y-gal4.
Marescotti M., Lagogiannis K., Webb B., Davies R.W, & Armstrong J.D. (2018). Monitoring brain activity and behaviour in freely moving Drosophila larvae using bioluminescence. Scientific Reports, 8, 9246.
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4

Drosophila Neurogenetics: Protocols and Stocks

Cited 3 times
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Flies were housed under standard conditions at 25 °C under a 12 hr light: 12 hr dark cycle. Fly stocks and sources: Drosophila melanogaster Canton-S, 20xUAS-IVS-GCaMP6s (Bloomington #42746, #42749), UAS-mCD8::GFP (#5130, #5137), LexAop-GCaMP6s (#53747), 10xUAS-IVS-myr::tdTomato (#32222), R71G01-Gal4 (#39599), AbdB-Gal4 (#55848) R25E04-Gal4 (#49125), and 20xUAS-IVS-CsChrimson.mVenus (#55134) were obtained from the Bloomington Stock Center. D. sechellia (14021-0248.25) and D. erecta (14021-0224.01) were obtained from the UCSD Stock Center. The following were gifts, obtained as indicated: D. ananassae and D. simulans (Richard Axel), D. simulans attP2039 36 (Yun Ding and David Stern, Janelia Research Campus); SplitP1-Gal4 24 (link) (David Anderson, Caltech); fruLexA37 (link) and fruGal416 (link) (Barry Dickson, HHMI/Janelia Farm Research Campus); D. melanogaster ppk23-Gal411 (link) (Kristin Scott, UC Berkeley); LexAop-myr::tdTomato (Cesar Mendes Columbia University); UAS-SPA-t2a-SPA-GFP 25 (link). Please see Supplemental Table 1 for detailed descriptions of genotypes used in each experiment.
Seeholzer L.F., Seppo M., Stern D.L, & Ruta V. (2018). Evolution of a central neural circuit underlies Drosophila mate preferences. Nature, 559(7715), 564-569.
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5

Drosophila Neuronal Characterization Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Flies were cultured in standard cornmeal food at 25°C in 12h/12h light/dark cycles. Fly stocks include: w1118, alrm-Gal426 (link), alrm-LexA::GAD, UAS-wtrw-gfp, LexAop-R-GECO1 (Bloomington, 52224), tdc2-Gal4 (Bloomington, 9313), 20XUAS-IVS-GCaMP6s (Bloomington, 42746), 13XLexAop2-IVS-GCaMP6s-p10 (Bloomington, 44274), UAS-Kir2.1-EGFP (Bloomington, 6595), UAS-Oct-TyrRRNAi (Bloomington, 28332), th-Gal4 (Bloomington, 8848), UAS-wtrwVDRC (VDRC, 107423), Oct-TyrRhono (Kyoto, 109038), UAS-shiDN (Bloomington, 5822), UAS-TrpA1 (Bloomington, 26263), UAS-NpHR (Kyoto, 117008), UAS-CsChrimson (Bloomington, 55135), AdoRKG03964ex (Bloomington, 30868), tdc2-LexA::p6527 (link), UAS-wtrw2.2328 (link), wtrwex29 (link), tβhnM1830 , tdc2RO5431 , th-LexA::p6532 , tsh-Gal8033 .
Ma Z., Stork T., Bergles D.E, & Freeman M.R. (2016). Neuromodulators signal through astrocytes to alter neural circuit activity and behavior. Nature, 539(7629), 428-432.
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6

Drosophila Genetic Toolbox Utilization

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We obtained 20XUAS-IVS-NES-jRCaMP1b-p10 (BL63793), 20XUAS-IVS-GCaMP6s (BL42746), 13XLexAop2-IVS-NES-jRCaMP1b-p10 (BL64428), and P{ST.lexA::HG}SJH-1 37 (BL66632, referred to as esg-LexA in this paper, Figures S1 andS2) from the Bloomington Stock Center. esg-GAL4 was obtained from the Kyoto Drosophila Genomics and Genetics Resource (DGRC). The following stocks were gifts: mex-GAL4 (Carl Thummel), esg-GFP[KI]/CyO (Norbert Perrimon), and pros-GAL4 (Sarah Siegrist). The complete key resources table can be found in Supplementary Information.
Kim A., Nguyen A., Marchetti M., Montell D.J., Pruitt B.L., & O’Brien L.E. (2021). Independently paced calcium oscillations in progenitor and differentiated cells in an ex vivo epithelial organ.
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