Tissue culture media, fetal bovine serum (FBS) and supplements were purchased from Gibco (Rockville, MD, USA). GPSAPDH was purchased from Cusabio (Wuhan, China). GNP, and FXT were purchased from Sigma (St. Louis, MO, USA). Anti-MMPs, anti-TIMPs, anti-uPA and uPA were purchased from Protein Tech (Chicago, IL, USA). Anti-ERK, anti-P-ERK, anti-JNK, anti-P-JNK, anti-p38, anti-P-p38, anti-AKT, and anti-P-AKT were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti- NF-κB, anti-c-Jun, and anti-c-Fos were purchased from Merck Milipore (Burlington, MA, USA). HRP-conjugated secondary antibodies were purchased from Bio-Rad (Madrid, Spain).
Anti upa
Manufactured by Proteintech
Sourced in United States, China
Anti-uPA is a laboratory reagent used for the detection and quantification of urokinase-type plasminogen activator (uPA) in various biological samples. It is a specific antibody that binds to uPA, allowing for its identification and measurement.
Automatically generated - may contain errors
Lab products found in correlation
Hrp conjugated secondary antibody, by Bio-Rad (1 mentions)
Anti erk, by Cell Signaling Technology (1 mentions)
Anti p erk, by Cell Signaling Technology (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Tissue culture media, by Thermo Fisher Scientific (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti p akt, by Cell Signaling Technology (1 mentions)
Anti c fos, by Merck Group (1 mentions)
Anti nf κb, by Merck Group (1 mentions)
Anti p p38, by Cell Signaling Technology (1 mentions)
Anti p38, by Cell Signaling Technology (1 mentions)
Anti c jun, by Merck Group (1 mentions)
Anti p jnk, by Cell Signaling Technology (1 mentions)
Anti jnk, by Cell Signaling Technology (1 mentions)
Ecl system, by Vazyme (1 mentions)
2 protocols using anti upa
1
Protein Expression and Signaling Pathway Analysis
2
Western Blot Analysis of Aortic Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Aortic tissues were washed with cold PBS and lysed in RIPA buffer to extract whole-cell protein, which was resolved by SDS-PAGE, transferred onto PVDF membrane, and blocked with 5% non-fat dry milk in TBS-T. The membrane was incubated with indicated primary antibody overnight at 4 °C, followed by incubation with a peroxidase-conjugated secondary antibody for 2 h, and finally developed with the ECL system (Vazyme Biotech Co.,Ltd). The western blotting results were quantified by densitometry and processed with Image J software (National Institutes of Health software). The following antibodies were used: anti-uPA (1:1000, #17968-1-AP, Proteintech Group, Inc.) and anti-PSMA4 (1:1000, RK05687, ABclonal Technology (Wuhan, China))
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