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Anti ikaros clone r32 1149

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Anti-IKAROS (clone R32-1149) is a laboratory reagent for research use. It is a monoclonal antibody that specifically binds to the IKAROS protein.

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Lab products found in correlation

Foxp3 staining kit, by Thermo Fisher Scientific (2 mentions) Anti cd3 clone ucht1, by BD (2 mentions) Anti cd19 clone sj25c1, by BD (2 mentions) Facscanto 2, by BD (2 mentions)

2 protocols using anti ikaros clone r32 1149

1

Analyzing Immune Cell Populations

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cryopreserved PBMCs were first stained with fluorescently labeled anti-CD3 (clone UCHT1) and anti-CD19 (clone SJ25C1; both from BD Biosciences). Afterward, cells were fixed and permeabilized with the FoxP3 staining kit (eBioscience), according to the manufacturer’s instructions, and incubated with fluorescently labeled anti-IKAROS (clone R32-1149; BD Biosciences).E4 (link) Cells were acquired on a FACSCanto II flow cytometer (BD Biosciences). Data were analyzed with FlowJo software (version X; TreeStar).
Bogaert D.J., Kuehn H.S., Bonroy C., Calvo K.R., Dehoorne J., Vanlander A.V., De Bruyne M., Cytlak U., Bigley V., De Baets F., De Baere E., Rosenzweig S.D., Haerynck F, & Dullaers M. (2017). A novel IKAROS haploinsufficiency kindred with unexpectedly late and variable B-cell maturation defects. The Journal of allergy and clinical immunology, 141(1), 432-435.e7.
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2

Analyzing Immune Cell Populations

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cryopreserved PBMCs were first stained with fluorescently labeled anti-CD3 (clone UCHT1) and anti-CD19 (clone SJ25C1; both from BD Biosciences). Afterward, cells were fixed and permeabilized with the FoxP3 staining kit (eBioscience), according to the manufacturer’s instructions, and incubated with fluorescently labeled anti-IKAROS (clone R32-1149; BD Biosciences).E4 (link) Cells were acquired on a FACSCanto II flow cytometer (BD Biosciences). Data were analyzed with FlowJo software (version X; TreeStar).
Bogaert D.J., Kuehn H.S., Bonroy C., Calvo K.R., Dehoorne J., Vanlander A.V., De Bruyne M., Cytlak U., Bigley V., De Baets F., De Baere E., Rosenzweig S.D., Haerynck F, & Dullaers M. (2017). A novel IKAROS haploinsufficiency kindred with unexpectedly late and variable B-cell maturation defects. The Journal of allergy and clinical immunology, 141(1), 432-435.e7.
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