Rabbit anti h2b

One microgram of vesicular proteins was separated by SDS-PAGE and then transferred to a 0.2 µm polyvinylidene difluoride membrane. The membrane was blocked with 5% non-fat milk or 3% skim milk in Tris-buffered saline with 0.05% Tween-20, incubated with primary antibody followed by secondary antibody conjugated with horseradish peroxidase, and subjected to the enhanced chemiluminescence. The membrane was washed three times by Tris-buffered saline with 0.05% Tween-20 after each incubation. Goat anti-actin (1:1000 dilution, SC-1616), rabbit anti-GAPDH (1:1000 dilution, SC-257,780), goat anti-mouse IgG (1:5000 dilution, SC-516,102), goat anti-rabbit IgG (1:5000 dilution, SC-2004), donkey anti-goat IgG (1:5000 dilution, SC-2020) and goat anti-rat IgG (1:5000 dilution, SC-2006) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Mouse anti-CD81 (1:1000 dilution, 555,675) and mouse anti-calnexin (1:1000 dilution, 610,523) antibodies were from BD Biosciences (San Diego, CA). Goat anti-ICAM1 (1:1000 dilution, BBA17) antibody was obtained from R&D Systems (Abingdon, UK). Mouse anti-60 S ribosomal protein L14 (RPL14) (1:1000 dilution, ab89095) antibody was from Abcam (Cambridge, MA). Mouse anti-tubulin (1:1000 dilution, T6074) antibody was from Sigma (St. Louis, MO). Rabbit anti-H2B (1:1000 dilution, 07–371) antibody was obtained from Millipore (Darmstadt, Germany).