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Anti mouse cd3 fitc or percp clone 145 2c11

Manufactured by Miltenyi Biotec
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Anti-mouse CD3-FITC or -PerCP (clone 145-2C11) is a fluorescently-labeled antibody that specifically binds to the CD3 protein on the surface of mouse T cells. This antibody can be used to identify and quantify T cells in flow cytometry analysis.

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Lab products found in correlation

Anti mouse foxp3 apc clone mf23, by BD (2 mentions) Facsdiva data analysis software, by BD (2 mentions)

Close spelling variants of this product

Anti-mouse CD3 FITC Anti-CD3 PerCP Anti-CD3-FITC CD3-PerCP CD3-FITC Anti-CD3

2 protocols using anti mouse cd3 fitc or percp clone 145 2c11

1

Multiparametric Flow Cytometry of T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Multi-parameter flow cytometry was used to determine T cell subpopulations and their activation status. Cells were stained with anti-mouse CD3-FITC or -PerCP (clone 145-2C11, Miltenyi Biotec), CD4-PacificBlue (clone RM4-5), CD8-APC-Cy7 (clone 53-6.7), CD69 – PE (clone H1.2F3) and anti-mouse Foxp3-APC (clone MF23) (BD Biosciences). All antibodies were diluted according to manufacturers’ instructions. Gating was set using an isotype-matched control antibody (Supplementary Figure 2). Stained cells were analyzed on a LSR II flow cytometer using the FACSDiva data analysis software (BD Biosciences).
Budiu R.A., Vlad A.M., Nazario L., Bathula C., Cooper K.L., Edmed J., Thaker P.H., Urban J., Kalinski P., Lee A.V., Elishaev E.L., Conrads T.P, & Flint M.S. (2016). Restraint and Social Isolation Stressors Differentially Regulate Adaptive Immunity and Tumor Angiogenesis in a Breast Cancer Mouse Model. Cancer and clinical oncology, 6(1), 12-24.
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2

Multiparametric Flow Cytometry of T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Multi-parameter flow cytometry was used to determine T cell subpopulations and their activation status. Cells were stained with anti-mouse CD3-FITC or -PerCP (clone 145-2C11, Miltenyi Biotec), CD4-PacificBlue (clone RM4-5), CD8-APC-Cy7 (clone 53-6.7), CD69 – PE (clone H1.2F3) and anti-mouse Foxp3-APC (clone MF23) (BD Biosciences). All antibodies were diluted according to manufacturers’ instructions. Gating was set using an isotype-matched control antibody (Supplementary Figure 2). Stained cells were analyzed on a LSR II flow cytometer using the FACSDiva data analysis software (BD Biosciences).
Budiu R.A., Vlad A.M., Nazario L., Bathula C., Cooper K.L., Edmed J., Thaker P.H., Urban J., Kalinski P., Lee A.V., Elishaev E.L., Conrads T.P, & Flint M.S. (2016). Restraint and Social Isolation Stressors Differentially Regulate Adaptive Immunity and Tumor Angiogenesis in a Breast Cancer Mouse Model. Cancer and clinical oncology, 6(1), 12-24.
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