1260 infinity 2 hplc

Molecular weights of Pg variants were determined using an Agilent 1260 Infinity II HPLC system connected to a Wyatt WTC-030S5 (7.6 nm × 300 nm, 5 μm, 300A°) SEC column. The SEC column was coupled to a mini DAWN Treos II light scattering detector and an Optilab T-rex differential refractive index detector (Wyatt Technology Corporation). Pg (100 μl) at a concentration of 1 mg/ml was injected into the SEC column at a flow rate of 0.5 ml/min using PBS, pH 7.4, as the running buffer. Light scattering and refractive index signals of eluted Pg peaks were recorded by respective detectors. The refractive index increment (dη/dc) was set to 0.185 ml/g. The data were analyzed for molar mass determinations using Astra 7.0 software (Wyatt Technology Corporation).

Full-length recombinant PfCSP was complexed with a molar excess of 850 Fab and loaded on a Superose 6 Increase 10/300 GL column (GE Healthcare) using an Agilent Technologies 1260 Infinity II HPLC coupled in-line with the following calibrated detectors: (i) MiniDawn Treos MALS detector (Wyatt); (ii) Quasi elastic light scattering (QELS) detector (Wyatt); and (iii) Optilab TreX refractive index (RI) detector (Wyatt). Data processing was performed using the ASTRA software (Wyatt).

Full-length recombinant PvCSPvk210 or PvCSPvk247 was complexed with a molar excess of 2F2 or 2E10.E9 Fab and loaded on a Superose 6 Increase 10/300 GL column (GE Healthcare) using an Agilent Technologies 1260 Infinity II HPLC coupled inline with the following calibrated detectors: (i) MiniDawn Treos MALS detector (Wyatt); (ii) quasi elastic light scattering (QELS) detector (Wyatt); and (iii) Optilab TreX refractive index (RI) detector (Wyatt). Data processing was performed using the ASTRA software (Wyatt).