Calcipotriol (R&D; Minneapolis, MN, USA) was dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich, St. Louis, MO, USA) at a concentration of 10 mM. TGF-β2 (R&D) was dissolved in sterile 4 mM HCl (Jiutai, Wenzhou, China) containing 0.1% bovine serum albumin (Gibco) at a concentration of 5 µg/mL. All stock reagents were aliquoted into smaller volumes and stored at −20°C for later use. The mice in different groups received daily intraperitoneal injections of the respective drugs without anesthesia using a microliter syringe attached to a 29-gauge needle. The visually unobstructed control mice received either DMSO (n = 11) or 0.1 mg/kg Calcipotriol (Cal; n = 10) or 0.5 mg/kg Cal (n = 11) in their respective experimental groups. Likewise, the FD mice also received either DMSO (n = 15) or 0.1 mg/kg Cal (n = 15) or 0.5 mg/kg Cal (n = 15) in their experimental groups. Calcipotriol doses (i.e., 0.1 mg/kg or 0.5 mg/kg body weight) were injected after dissolving them in DMSO.
Calcipotriol
Manufactured by R&D Systems
Sourced in United States
Calcipotriol is a synthetic vitamin D derivative. It is commonly used in research applications to study the effects of vitamin D and its analogs on various biological processes.
Automatically generated - may contain errors
2 protocols using calcipotriol
1
Calcipotriol Dosage Effects on FD Mice
2
Topical Calcipotriol and AMG 487 in Mouse Skin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MC903 (Calcipotriol; R and D Systems) was applied to the shaved mouse cheek (20 μl of 0.2 mM in ethanol) or rostral back (40 µl of 0.2 mM in ethanol) once per day for 1–12 days using a pipette. 100% ethanol was used. All MC903 studies were performed on 8–12 week old age-matched mice. Behavior, RNA-seq, flow cytometry, and immunohistochemistry were performed on days 1, 2, 3, 5, eight and/or 12. For AMG 487 experiments in the MC903 model, 50 µL 3.31 mM AMG 487 (Tocris) or 20% HPCD-PBS vehicle was injected subcutaneously one hour prior to recording behavior (Qu et al., 2015 (link)). Spontaneous scratching was manually scored for the first 30 min of observation. Both bout number and length were recorded. Behavioral scoring was performed while blind to experimental condition and mouse genotype.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!