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Hpa002018

Manufactured by Merck Group
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HPA002018 is a laboratory instrument designed for DNA and RNA extraction and purification. It utilizes a magnetic bead-based separation technology to efficiently isolate nucleic acids from a variety of sample types. The core function of HPA002018 is to provide a reliable and automated solution for the extraction and purification of genetic material for further analysis and experimentation.

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Lab products found in correlation

Lsm 510, by Zeiss (2 mentions) Fabppm, by Novus Biologicals (2 mentions) Fatp1, by Novus Biologicals (2 mentions) Fatp 4, by Abnova (2 mentions) Wheat germ agglutinin alexa fluor 594 conjugate, by Thermo Fisher Scientific (2 mentions) Lsm image browser, by Zeiss (2 mentions) Axiovert 100 microscope, by Zeiss (2 mentions)

2 protocols using hpa002018

1

Subcellular Localization of Fatty Acid Transporters

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were plated on glass coverslips, fixed with 4% paraformaldehyde (PFA) and incubated overnight at 4°C with primary antibodies against CD36 (rabbit, HPA002018, 1:200, Sigma-Aldrich), FABPpm (mouse, 1:200, Novus Biologicals) FATP1 (mouse, 1:200, Novus Biologicals), FATP-4 (mouse, 1:200, Abnova). For plasma membrane labeling, Alexa Fluor 594 wheat germ agglutinin conjugate (W11262, Molecular Probes, Eugene, OR) was used. Samples were stained with Alexa Fluor 488 (A11059, A11001 and A11008, 1:2,000, Molecular Probes) conjugated secondary antibodies. After nuclei counterstain with Hoechst, coverslips were imaged with an inverted laser scanning confocal microscope (Zeiss LSM 510 equipped with a 63× oil immersion objective, Thornwood, NY). Z-stack confocal images were taken at 30-s intervals using AIM (version 3.2, Zeiss), and images were compiled using Zeiss LSM Image Browser (versions, 4.2, Zeiss). For epifluorescent microscopy, images were taken with a Zeiss Axiovert 100 microscope.
Ladanyi A., Mukherjee A., Kenny H.A., Johnson A., Mitra A.K., Sundaresan S., Nieman K.M., Pascual G., Benitah S.A., Montag A., Yamada S.D., Abumrad N.A, & Lengyel E. (2018). Adipocyte-induced CD36 expression drives ovarian cancer progression and metastasis. Oncogene, 37(17), 2285-2301.
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2

Subcellular Localization of Fatty Acid Transporters

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were plated on glass coverslips, fixed with 4% paraformaldehyde (PFA) and incubated overnight at 4°C with primary antibodies against CD36 (rabbit, HPA002018, 1:200, Sigma-Aldrich), FABPpm (mouse, 1:200, Novus Biologicals) FATP1 (mouse, 1:200, Novus Biologicals), FATP-4 (mouse, 1:200, Abnova). For plasma membrane labeling, Alexa Fluor 594 wheat germ agglutinin conjugate (W11262, Molecular Probes, Eugene, OR) was used. Samples were stained with Alexa Fluor 488 (A11059, A11001 and A11008, 1:2,000, Molecular Probes) conjugated secondary antibodies. After nuclei counterstain with Hoechst, coverslips were imaged with an inverted laser scanning confocal microscope (Zeiss LSM 510 equipped with a 63× oil immersion objective, Thornwood, NY). Z-stack confocal images were taken at 30-s intervals using AIM (version 3.2, Zeiss), and images were compiled using Zeiss LSM Image Browser (versions, 4.2, Zeiss). For epifluorescent microscopy, images were taken with a Zeiss Axiovert 100 microscope.
Ladanyi A., Mukherjee A., Kenny H.A., Johnson A., Mitra A.K., Sundaresan S., Nieman K.M., Pascual G., Benitah S.A., Montag A., Yamada S.D., Abumrad N.A, & Lengyel E. (2018). Adipocyte-induced CD36 expression drives ovarian cancer progression and metastasis. Oncogene, 37(17), 2285-2301.
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