Nutlin 3 s1061

Between April 2007 and April 2008, we recruited 146 patients with primary GC from the Zhongshan hospital and Shanghai Cancer Center, Fudan University. All the patients (excluding the stage IV disease) underwent an R0 resection (with no tumour cells at the margin) with D2 lymph node dissection, and all the patients histopathologically confirmed for the diagnosis of stages II and III received 6–8 courses of chemotherapy-based fluorouracil following the operation. The Ethics Committee of Fudan University approved our study. All the participants provided written informed consent prior to enrolment. The human GC cell lines were obtained from the American Type Culture Collection (Manassas, VA, USA) and were identified through an STR analysis. The p53 antibody (#9282) and p21 antibody (#8831) were purchased from Cell Signaling Technology (Beverly, MA, USA). Nutlin-3 (S1061) was purchased from Selleck (Princeton, NJ, USA).

Anti-p53 antibodies (DO-1) (sc-126) and p21 antibodies (SX118) (sc-53870) were obtained from Santa Cruz Biotechnology (Santa Cruz Biotechnology, Dallas, TX, USA); Anti-PARP (9532 S), anti-Caspase-3 (9662S), anti-Mouse, (7076S) and anti-Rabbit (7074S) antibodies were from Cell Signaling Technology (Cell Signaling Technology, Danvers, MA, USA); and anti-α Tubulin (10D8) was obtained from Santa Cruz Technology (Santa Cruz Technology, Dallas, TX, USA). SAR405838 (S7649) and Nutlin-3 (S1061) was purchased from Selleckchem (Selleckchem, Houston, USA). RG7388 (A3763) was from APExBIO (APExBIO, Houston, TX, USA). Doxorubicin (Dox, D1515) was from Sigma (Sigma-Aldrich Corp, St. Louis, MO, USA).