Resonance Raman measurements with a spectral resolution of 1 cm−1 were performed as described previously.15 (link) Briefly, the 413.1 nm output from a Kr ion laser (Spectra Physics, Mountain View, CA) was focused to an ~30 μm spot on a spinning quartz cell (~6000 rpm). The scattered light was collected at a 90° angle and focused on the 100 μm wide slit of a 1.25 m Spex spectrometer equipped with a 1200 grooves/mm grating (Horiba Jobin Yvon, Edison, NJ), where it was dispersed and detected by a CCD detector (Princeton Instruments, Trenton, NJ). Rayleigh scattering was removed by a holographic notch filter (Kaiser, Ann Arbor, MI). The Raman shifts were calibrated by using indene. The laser power was kept at ~5 mW. The concentrations of CYP101D1 and Arx were 50 μM each.
Ccd detector
Manufactured by Teledyne
The CCD detector is a device used for capturing and converting light into electrical signals. It functions by using a semiconductor material to generate an electric charge in response to the absorption of photons. This charge is then read out and processed, allowing the detector to capture images or make precise measurements of light intensity.
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Lab products found in correlation
4 protocols using ccd detector
1
Resonance Raman Spectroscopy of CYP101D1 and Arx
2
SHG Characterization of CdS Nanobelt Devices
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The CdS nanobelt devices were mounted in a continuous flow optical microscopy cryostat (ST-500, Janis research) with electrical feedthroughs connected to an electrical measurement system, for room-temperature and low-temperature measurements. The voltage bias was sourced (0–200 V) and the output current signal was converted to an amplified voltage signal by a current preamplifier (DL instruments model 1211) and recorded continuously (~10 data points per second) by PCI card (National Instrument, NI PCI-6281). A femtosecond pulsed Ti: sapphire laser (Chameleon), tuned from 680 to 1080 nm with ~140 fs pulse width and 80 MHz repetition rate, was used to perform SHG measurements. The laser polarization was controlled by a half-wave plate (HWP) and then focused (spot size ~3 μm) onto individual nanobelts by a home-built microscope equipped with a ×60, 0.7 NA objective (Nikon). The back-scattered SHG signals were imaged by a cooled charge-coupled device (CCD) and detected by a spectrometer (Acton) with a 300 groove mm−1 500 nm blaze grating with a CCD detector (Princeton instruments) with a spectral resolution of 0.1 nm.
3
High-Pressure Raman Spectroscopy of Samples
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The DAC consists
of two opposing type IA ultralow fluorescence diamond anvils with
a 500 μm culet. Samples were loaded into a hole with a diameter
of 180 μm drilled in a stainless steel gasket preindented to
a thickness between 50 and 60 μm. Silicone oil was used as a
PTM. Pressure was calibrated by the fluorescence emission of ruby
in the sample chamber. The Raman spectra were measure using a Horiba
Jobin Yvon HR800 confocal spectrometer. Before detecting the Raman
spectra, the spectrometer was calibrated by the standard Raman peak
of a silicon wafer at 520.7 cm–1. Raman signals
that were excited with 473 nm laser were recorded by means of Princeton
Instruments CCD detector and were collected in the 200–2400
cm–1 frequency range. The Rayleigh scattering were
removed using a holographic notch filter. In every pressure experiment
performed, it was necessary to wait 15 min to reach a steady state.
of two opposing type IA ultralow fluorescence diamond anvils with
a 500 μm culet. Samples were loaded into a hole with a diameter
of 180 μm drilled in a stainless steel gasket preindented to
a thickness between 50 and 60 μm. Silicone oil was used as a
PTM. Pressure was calibrated by the fluorescence emission of ruby
in the sample chamber. The Raman spectra were measure using a Horiba
Jobin Yvon HR800 confocal spectrometer. Before detecting the Raman
spectra, the spectrometer was calibrated by the standard Raman peak
of a silicon wafer at 520.7 cm–1. Raman signals
that were excited with 473 nm laser were recorded by means of Princeton
Instruments CCD detector and were collected in the 200–2400
cm–1 frequency range. The Rayleigh scattering were
removed using a holographic notch filter. In every pressure experiment
performed, it was necessary to wait 15 min to reach a steady state.
4
Resonance Raman Spectroscopy of Heme Proteins
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