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Ix81 zdc zero drift microscope

Manufactured by Teledyne
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Sourced in United States

The IX81-ZDC zero drift microscope is a high-performance optical microscope designed for precise and stable imaging. It features advanced technology to maintain focus and alignment, ensuring consistent and reliable data acquisition.

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Lab products found in correlation

Cascade 512b camera, by Teledyne (2 mentions)

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IX81 microscope

2 protocols using ix81 zdc zero drift microscope

1

Hypoxia-Induced Cell Death Assay

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Cells were transfected with NT or BICD1 siRNA for 24 h and then incubated with 0.1 μg/mL of PI-supplied SR media in a live-cell imaging chamber (Tokai, Tokyo, Japan). Normoxic (5% CO2, 21% O2, and 74% N2) or hypoxic (5% CO2, 0.5% O2, and 94.5% N2) gas was supplied to chamber for 48 h. Differential interference contrast images and red fluorescence protein images were acquired over 72 h at 6 h intervals with an Olympus IX81-ZDC zero drift microscope and a Cascade 512 B camera (Roper Scientific, Tucson, AZ, USA). The number of PI-positive cells was determined with the ImageJ software.
Lee H.J., Jung Y.H., Oh J.Y., Choi G.E., Chae C.W., Kim J.S., Lim J.R., Kim S.Y., Lee S.J., Seong J.K, & Han H.J. (2018). BICD1 mediates HIF1α nuclear translocation in mesenchymal stem cells during hypoxia adaptation. Cell Death and Differentiation, 26(9), 1716-1734.
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2

Hypoxia Impacts on Stem Cell Colonies

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After glucosamine or LPA treatment, the culture dish was placed in a temperature and CO2 control chamber (Tokai, Tokyo, Japan). Normoxic or hypoxic gas was supplied for 48 h. Differential interference contrast images were acquired over those 48 h at 10 min intervals by using an Olympus IX81-ZDC zero-drift microscope and a Cascade 512B camera (Roper Scientific, Tucson, AZ, USA). A constant threshold of each image was maintained. Determination of the mESC colony area was performed by using ImageJ software.
Lee H.J., Ryu J.M., Jung Y.H., Lee K.H., Kim D.I, & Han H.J. (2016). Glycerol-3-phosphate acyltransferase-1 upregulation by O-GlcNAcylation of Sp1 protects against hypoxia-induced mouse embryonic stem cell apoptosis via mTOR activation. Cell Death & Disease, 7(3), e2158-.
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