Illuminator hxp 200c

Manufactured by Zeiss

The Illuminator HXP 200C is a compact, high-intensity light source designed for fluorescence microscopy applications. It features a 200-watt mercury vapor lamp that provides powerful, broad-spectrum illumination for various fluorescent dyes and probes. The illuminator is designed for easy integration into microscope systems, offering convenient and reliable performance for a wide range of research and imaging needs.

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Lab products found in correlation

Axio zoom v16, by Zeiss (8 mentions) Axio observer z1, by Zeiss (6 mentions) Axio examiner d1 microscope, by Zeiss (5 mentions) Andor ixon ultra, by Oxford Instruments (5 mentions) Andor solis software, by Oxford Instruments (5 mentions) Lsm 880, by Zeiss (4 mentions) Epiplan neo uar 50x 0.55 dic, by Zeiss (3 mentions) Fl filter set 38 he gfp, by Zeiss (3 mentions) Fl filter set 38 he gfp shift free, by Zeiss (2 mentions) Orca flash4.0 lt, by Zeiss (2 mentions) Colibri 2 led light, by Zeiss (2 mentions) Zen system, by Zeiss (2 mentions) Plan apochromat objective, by Zeiss (2 mentions) Thermo plate, by Tokai Hit (2 mentions) Ec epiplan neofluar 10x 0, by Zeiss (1 mentions) Nis elements advanced research software, by Nikon (1 mentions) Axiocam 506 mono camera, by Zeiss (1 mentions) Zen software, by Zeiss (1 mentions) Axiocam 506 monochrome camera, by Zeiss (1 mentions)

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HXP 200C (10 citations)

9 protocols using illuminator hxp 200c

High-Resolution Live-Cell Imaging

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Imaging was performed through a 50× objective (LD EC Epiplan-Neofluar 50×/0.55 DIC) mounted on a Zeiss Axio Examiner D1 microscope. A Zeiss Illuminator HXP 200C light source and an eGFP filter cube (FL Filter Set 38 HE GFP shift free) were used for fluorescence.
An EMCCD camera (Andor iXon Ultra, Oxford Instruments) using Andor Solis software (Oxford Instruments) was used to record videos of 20 s at 512 × 512 pixel resolution. The exposure time was 200 ms (5 Hz).

Afify A, & Potter C.J. (2020). Insect repellents mediate species-specific olfactory behaviours in mosquitoes. Malaria Journal, 19, 127.

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Anesthetized Pup Head-Fixed Imaging

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After 1 hr of post-surgical recovery from anesthesia, pups were moved into a swaddling 15 mL conical centrifuge tube. The top half of this tube was removed to allow access to the headbar and visualization of the midbrain. Pups were head-fixed and maintained at 37°C using a heating pad and temperature controller (TC-1000; CWE). During the experiments, pups were generally immobile; however, occasional limb and tail twitching did occur.
For wide field epifluorescence imaging, images were captured at 10 Hz using a Hamamatsu ORCA-Flash4.0 LT digital CMOS camera attached to a Zeiss Axio Zoom.V16 stereo zoom microscope. A 4 × 4 mm field of view was illuminated continuously with a metal halide lamp (Zeiss Illuminator HXP 200C) and visualized through a 1X PlanNeoFluar Z 1.0x objective at 17x zoom. Images were captured at a resolution of 512 × 512 pixels (16-bit pixel depth) after 2 × 2 binning to increase sensitivity. Each recording consisted of uninterrupted acquisition over 10 min or 20 min if injected with pharmacological agents.

Babola T.A., Kersbergen C.J., Wang H.C, & Bergles D.E. (2020). Purinergic signaling in cochlear supporting cells reduces hair cell excitability by increasing the extracellular space. eLife, 9, e52160.

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Neonatal Rodent Imaging of Midbrain

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After 1 hour of post-surgical recovery from anesthesia, pups were moved into a swaddling 15 mL conical centrifuge tube. The top half of this tube was removed to allow access to the headbar and visualization of the midbrain or midbrain and caudal part of the cortex. Pups were head-fixed and maintained at 37°C using a heating pad and temperature controller (TC-1000; CWE). During the experiments, pups were generally immobile; however, occasional limb and tail twitching did occur.
For wide field epifluorescence imaging, images were captured at 10 Hz using a Hamamatsu ORCA-Flash4.0 LT digital CMOS camera attached to a Zeiss Axio Zoom.V16 stereo zoom microscope. A 4 x 4 mm field of view was illuminated continuously with a mercury lamp (Zeiss Illuminator HXP 200C) and visualized through a 1X PlanNeoFluar Z 1.0x objective at 17x zoom. Images were captured at a resolution of 512 x 512 pixels (16-bit pixel depth) after 2 x 2 binning to increase sensitivity. Each recording consisted of uninterrupted acquisition over 30 minutes or 40 minutes if injected with pharmacological agents.

Babola T.A., Li S., Wang Z., Kersbergen C.J., Elgoyhen A.B., Coate T.M., & Bergles D.E. (2020). Purinergic signaling controls spontaneous activity in the auditory system throughout early development.

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Live-Cell Fluorescence Microscopy

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Imaging was performed through a 50x objective (LD EC Epiplan-Neo uar 50x/0.55 DIC) mounted on a Zeiss Axio Examiner D1 microscope. A Zeiss Illuminator HXP 200C light source and an eGFP lter cube (FL Filter Set 38 HE GFP shift free) were used for uorescence.
An EMCCD camera (Andor iXon Ultra, Oxford Instruments) using Andor Solis software (Oxford Instruments) was used to record videos of 20 seconds at 512x512 pixel resolution. The exposure time was 200 ms (5 Hz).

Afify A., & Potter C.J. (2020). Insect Repellents Mediate Species-Specific Olfactory Behaviors in Mosquitoes.

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Live-Cell Fluorescence Microscopy

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Afify A., & Potter C.J. (2020). Insect Repellents Mediate Species-Specific Olfactory Behaviours in Mosquitoes.

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Live-Cell Fluorescence Microscopy

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Afify A., & Potter C.J. (2020). Insect Repellents Mediate Species-Specific Olfactory Behaviors in Mosquitoes.

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Fluorescent Antennae Imaging Protocol

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Antennae were imaged through a 10x (Zeiss EC Epiplan-Neofluar 10x/0.25) or a 50x (LD EC Epiplan-Neofluar 50x/0.55 DIC) objective mounted on a Zeiss Axio Examiner D1 microscope. For fluorescence, a light source (Zeiss Illuminator HXP 200C) and eGFP filter cube (FL Filter Set 38 HE GFP shift free) were used.
For image acquisition, an EMCCD camera (Andor iXon Ultra, Oxford Instruments), NIS Elements Advanced Research software (Nikon instruments), and Andor Solis software (Oxford Instruments) were used. Recordings were for 20 seconds, at a resolution of 512×512 pixels, and an exposure time of 200 ms (5 Hz).

Afify A., Betz J.F., Riabinina O., Lahondère C, & Potter C.J. (2019). Commonly used insect repellents hide human odors from Anopheles mosquitoes. Current biology : CB, 29(21), 3669-3680.e5.

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Confocal Microscopy Imaging Techniques

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The confocal laser scanning microscope (CLSM) LSM880 (Carl Zeiss, Jena, Germany) equipped with a 63×/0.9 numerical aperture Plan-Apochromat objective was used to acquire confocal microscopic images. We visualize fungal and rice cells by CLSM and confocal reflection microscopy. Fungal nuclei and rice cells were visualized with 488 and 400-nm lasers, respectively. Acquired confocal images were analyzed using ZEN Software (Version 3.5, Carl Zeiss) and ImageJ software. Another CLSM TCS SP5 (Leica, Mannheim, Germany) equipped with HC PL APO 20x/0.75 IMM CORR CS2 objective lens was used to acquire confocal dual-color microscopic images. For epi-fluorescent inverted microscopy, cells were observed using an Axio Observer Z1 (Carl Zeiss) microscope equipped with a Plan-Apochromat 63  ×  1.4 Oil or 10 or 20 times objective lens, an AxioCam 506 mono camera and Colibri.2 LED light (Carl Zeiss). Temperature of the stage was kept at 30 °C by a thermo-plate (TOKAI HIT, Japan). For zoom microscopy, plates were observed by AXIO Zoom V16 and HXP 200C illuminator (Carl Zeiss). Images were collected and analyzed using the Zen system (Carl Zeiss) and ImageJ software.

Yasui M., Oda K., Masuo S., Hosoda S., Katayama T., Maruyama J.I., Takaya N, & Takeshita N. (2020). Invasive growth of Aspergillus oryzae in rice koji and increase of nuclear number. Fungal Biology and Biotechnology, 7, 8.

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Confocal Microscopy for Bacterial and Fungal Imaging

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A confocal laser scanning microscope LSM880 (Carl Zeiss) equipped with a 63×/0.9 numerical aperture Plan-Apochromat objective and a 40×/0.75 numerical aperture IR Achroplan W water immersion objectives (Carl Zeiss), were used to acquire confocal microscopic images. Bacteria and/or fungi were irradiated with 488- and 633-nm lasers to detect the Green fluorescent protein (ZsGreen) and reflected light, respectively. Acquired confocal images were analyzed using ZEN Software (Version 3.5; Carl Zeiss) and ImageJ software. Another confocal laser scanning microscope TCS SP8 Tandem scanner 8 kHz (Leica) equipped with HC PL APO 20×/0.75 IMM CORR CS2 objective lens was used to acquire high-speed confocal dual-color microscopic images. Bacteria and fungi were irradiated with 488- and 522-nm lasers to detect the GFP and mCherry, respectively. Epi-fluorescent inverted microscopy: cells were observed with an Axio Observer Z1 (Carl Zeiss) microscope equipped with a Plan-Apochromat 63 × 1.4 Oil or 10 or 20 times objective lens, an AxioCam 506 monochrome camera and Colibri.2 LED light (Carl Zeiss). Temperature of the stage was kept at 30°C by a thermo-plate (TOKAI HIT). Using zoom microscopy, plates were observed by AXIO Zoom V16 and HXP 200C illuminator (Carl Zeiss). Images were collected and analyzed using the Zen system (Carl Zeiss) and ImageJ software.

Abeysinghe G., Kuchira M., Kudo G., Masuo S., Ninomiya A., Takahashi K., Utada A.S., Hagiwara D., Nomura N., Takaya N., Obana N, & Takeshita N. (2020). Fungal mycelia and bacterial thiamine establish a mutualistic growth mechanism. Life Science Alliance, 3(12), e202000878.

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