Tac1cre

Manufactured by Jackson ImmunoResearch

Tac1Cre is a Cre recombinase expressed under the control of the Tac1 promoter. Cre recombinase is an enzyme that catalyzes the site-specific recombination of DNA between loxP sites, allowing for genetic manipulation in transgenic animal models.

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2 protocols using tac1cre

Transgenic Mouse Models for Neuroscience

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Animal studies were conducted following Institutional Animal Care and Use Committee guidelines and protocols. RiboTag, Esr1^Cre, SunTag, and Tac1^Cre mice were purchased from Jackson Labs and bred in our mouse colony. We engineered Esr1^Flpo, PR^Flpo, and Cckar^Cre mice as described below and bred them in our mouse colony. Mice were housed under a reversed 12:12 hr light:dark cycle. Food and water were available ad libitum. Wild-type C57BL6/J males and females used for ISH were purchased from Jackson Laboratories. WT females were purchased from Jax (C57B16/J), and WT males (B6129SF1/J) used as stimulus animals in tests of sexual receptivity were bred in the animal facility or purchased from Jackson Laboratories. All mice were group housed by sex after weaning. Group housed mice 10–12 weeks of age were used for deep sequencing and ISH studies. For behavioral studies, mice were 12–16 weeks of age at the time of testing. Male mice used for behavioral testing were single housed for ≥1 week prior to testing. Female mice used for mating assays were group-housed.

Knoedler J.R., Inoue S., Bayless D.W., Yang T., Tantry A., Davis C.H., Leung N.Y., Parthasarathy S., Wang G., Alvarado M., Rizvi A.H., Fenno L.E., Ramakrishnan C., Deisseroth K, & Shah N.M. (2022). A functional cellular framework for sex and estrous cycle-dependent gene expression and behavior. Cell, 185(4), 654-671.e22.

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Genetic mouse models for neuroscience

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Animal experiments were approved by The Rockefeller University Animal Care and Use Committee and were carried out in accordance with the National Institutes of Health guidelines. All experiments were conducted on male mice older than 8 weeks of age housed in a 12-h light/12-h dark cycle at 22 °C and 30–60% humidity. We used the following genotypes of mice: C57BL/6J (wild type; number 000664, The Jackson Laboratory), FOS^2A-iCreER (TRAP2) mice (The Jackson Laboratory, stock number 030323), CAG–Sun1/sfGFP (INTACT) mice (The Jackson Laboratory, stock number 021039), VGLUT2–IRES–Cre mice (The Jackson Laboratory, stock no. 028863), VGAT–IRES–Cre mice (The Jackson Laboratory, stock no. 028862), ADCYAP1–2A–Cre mice (The Jackson Laboratory, stock number 030155), PHOX2B–Cre (The Jackson Laboratory, Stock 016223), DBH–Cre (The Jackson Laboratory, stock number 033951), TH–Cre (The Jackson Laboratory, stock number 008601), TAC1–Cre (The Jackson Laboratory, stock number 021877), PDYN–Cre (The Jackson Laboratory, stock number 027958), CCK–Cre (The Jackson Laboratory, stock number 012706), NTS–Cre (The Jackson Laboratory, stock number 017525). For all Cre mouse line experiments, only heterozygous animals were used. Sample sizes were decided based on experiments from similar studies. Animals were randomly assigned to groups when possible.

Ilanges A., Shiao R., Shaked J., Luo J.D., Yu X, & Friedman J.M. (2022). Brainstem ADCYAP1+ neurons control multiple aspects of sickness behaviour. Nature, 609(7928), 761-771.

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