Mouse monoclonal anti irp1

Unless otherwise stated, all chemicals, including aspirin (ASA), LPS (Escherichia coli 055:B5), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), and mouse monoclonal anti-β-actin, were obtained from Sigma Chemical Co., St. Louis, MO, USA. A BCA protein assay kit and a Revert Aid First Strand cDNA Synthesis Kit were purchased from Thermo Scientific, Waltham, MA, USA, and TRIZOL Reagent from Life technologies, Carlsbad, CA, USA. Rabbit monoclonal anti-phospho-JAK2, rabbit monoclonal anti-JAK2, rabbit polyclonal anti-phospho-STAT3, mouse monoclonal anti-STAT3, rabbit monoclonal anti-phospho-P65, and rabbit monoclonal anti-P65 antibodies were supplied by Cell Signaling Technology, Inc., Danvers, MA, USA, and mouse monoclonal anti-IRP1 was from Abcam, San Francisco, CA, USA. Goat anti-rabbit and anti-mouse IRDye 800CW secondary antibodies were bought from LI-COR bio sciences, Lincoln, NE, USA. The Health Department of Hong Kong and Shanghai Government and the Animal Research Ethics Committee of The Chinese University of Hong Kong (the project identification code: GRF14106914, 1 January 2015) and Fudan University (the project identification code: NSFC31271132, 1 January 2013; NSFC31330035, 1 January 2014) approved the experimental procedures of this study.