Fluorescence images were taken of live parasites on a Leica D6B fluorescence microscope equipped with a Leica DFC9000 GT camera and a Leica Plan Apochromat 60× or 100× / 1.4 oil objective. Contrast and intensities were linear adjusted to present clear parasite shapes using Fiji and cropped images were assembled into a composition of cells for Fig. 1 using Adobe Illustrator CC 2021.
D6b fluorescence microscope
Manufactured by Leica
The Leica D6B is a fluorescence microscope designed for high-performance imaging. It features advanced optics and illumination systems to provide clear, detailed images of fluorescently labeled samples. The D6B is capable of capturing high-quality data for a variety of research and analysis applications.
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Lab products found in correlation
Dfc9000 gt camera, by Leica (2 mentions)
Illustrator cc 2021, by Adobe (1 mentions)
Dm2000 led light microscope, by Leica (1 mentions)
Dmc2900 camera, by Leica (1 mentions)
Eclipse ni light microscope, by Nikon (1 mentions)
C11440 camera, by Hamamatsu Photonics (1 mentions)
Dfc9000gt, by Leica (1 mentions)
Cellrox orange, by Thermo Fisher Scientific (1 mentions)
4 protocols using d6b fluorescence microscope
1
Fluorescence Imaging of Live Parasites
2
Quantitative Analysis of Fluo-CQ Uptake
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Giemsa-stained culture smears were imaged on a DM2000 LED light microscope (Leica) equipped with a DMC2900 camera (Leica). Fluorescence microscopy was performed with an Eclipse Ni light microscope (Nikon) fitted with a C11440 camera (Hamamatsu) or with a D6B fluorescence microscope (Leica) equipped with a DFC9000 GT camera (Leica). For detailed microscopy protocols including quantitative analysis of Fluo-CQ uptake, please refer to SI Appendix .
3
Live-cell Nuclei Staining Protocol
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For live-cell microscopy, LCN-Kd parasites were incubated with 1 mg/ml DAPI in culture medium for 15 min at 37 C to stain nuclei. Parasites were then washed once in PBS and imaged in PBS on a Leica D6B fluorescence microscope equipped with a Leica DFC9000 GT camera and a Leica Plan Apochromat 100x/1.4 oil objective. Image processing was performed using ImageJ (Schneider et al., 2012) .
4
Oxidative Stress Measurement in Malaria Parasites
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For measurement of oxidative stress levels, LCN-Kd and LCN-Con ring stage parasites were treated with 2.5 mM glucosamine or were left untreated. At around 30 hpi, trophozoite stage parasites were stained with 5 mM CellROX Orange (Thermo Fisher Scientific) in culture medium for 30 min at 37 C. After three washes with PBS, they were directly imaged on a Leica D6B fluorescence microscope as described above. Mean fluorescence intensities and size (area) of individual parasites were determined using the ROI manager of ImageJ (Schneider et al., 2012) .
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