After rehydration and antigenic retrieval in citrate buffer (10 mM , pH 6.1), the tissue sections were stained for ER (clone 6F11, Novocastra, Leica Biosystems, Newcastle, UK; 1/200) and PR (clone 1A6, Novocastra, 1/200). Revelation of staining was performed using the Vectastain Elite ABC peroxidase mouse IgG kit (Vector, Burlingame, CA, USA) and diaminobenzidine (Dako A/S, Glostrup, Denmark) as chromogen. Positive and negative controls were included in each slide run. Cases were considered positive for ER and PR according to the standardised guidelines using a cutoff of ⩾10% stained tumour nuclei (Balaton et al, 1995 (link), 1996 (link)).
Er clone 6f11
Manufactured by Leica
Sourced in United Kingdom
ER (clone 6F11) is a laboratory equipment product from Leica. It is used for the detection of estrogen receptor (ER) proteins. The core function of this product is to enable the immunohistochemical identification and localization of ER proteins in tissue samples.
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Lab products found in correlation
2 protocols using er clone 6f11
1
Immunohistochemical Analysis of ER and PR
2
Immunohistochemical Evaluation of ER and PR
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After rehydration and antigenic retrieval in citrate buffer (10 mM, pH 6.1), the tissue sections were stained for ER (ER, clone 6F11, Novocastra, 1/200), and PR (PR, clone 1A6, Novocastra, 1/200). Revelation of staining was performed using the Vectastain Elite ABC peroxidase mouse IgG kit (Vector Burlingame, CA) and diaminobenzidine (Dako A/S, Glostrup, Denmark) as chromogen. Positive and negative controls were included in each slide run. Cases were considered positive for ER and PR according to standardized guidelines using a cut-off of ≥10% stained tumour nuclei.
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