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Anti pir pigfr y1162 y1163 ab

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Anti-pIR/pIGFR-Y1162/Y1163 Ab is a laboratory reagent used to detect and quantify phosphorylation of the insulin receptor (IR) and insulin-like growth factor receptor (IGFR) at specific tyrosine residues (Y1162 and Y1163). This antibody can be used in various immunochemical techniques, such as Western blotting, to study the activation and signaling of these receptors.

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Lab products found in correlation

Anti pakt s473, by Cell Signaling Technology (2 mentions) Anti p erk t202 y204, by Cell Signaling Technology (2 mentions) Anti pakt t308, by Cell Signaling Technology (2 mentions) Anti gapdh clone d16h11xp, by Cell Signaling Technology (2 mentions) Anti irβ ab, by Santa Cruz Biotechnology (2 mentions) Hrp conjugated anti rabbit and anti mouse igg abs, by GE Healthcare (2 mentions)

2 protocols using anti pir pigfr y1162 y1163 ab

1

Western Blot Antibody Specifications

Check if the same lab product or an alternative is used in the 5 most similar protocols
The anti-LMPTP antibody (Ab) was described in 23 . The anti-GAPDH (clone D16H11XP; catalog #5174), anti-pAKT-T308 (catalog #9275), anti-pAKT-S473 (catalog #9271) and anti-pERK-T202/Y204 (catalog #9101) Abs were purchased from Cell Signaling Technology. The anti-IRβ Ab was purchased from Santa Cruz Biotechnology (clone C-19; catalog #sc-711) and the anti-pIR/pIGFR-Y1162/Y1163 Ab was purchased from Life Technologies (catalog #44-804G). Primary antibodies were used at 1:1000 dilution for Western blotting. HRP-conjugated anti-rabbit and anti-mouse IgG Abs were purchased from GE Healthcare (catalog #NA934-1ML and NA931-1ML respectively) and used at 1:3000 dilution. Unless otherwise specified, chemicals and all other reagents were purchased from Sigma-Aldrich.
Stanford S.M., Aleshin A.E., Zhang V., Ardecky R.J., Hedrick M.P., Zou J., Ganji S.R., Bliss M.R., Yamamoto F., Bobkov A.A., Kiselar J., Liu Y., Cadwell G.W., Khare S., Yu J., Barquilla A., Chung T.D., Mustelin T., Schenk S., Bankston L.A., Liddington R.C., Pinkerton A.B, & Bottini N. (2017). Diabetes reversal by inhibition of the low molecular weight tyrosine phosphatase. Nature chemical biology, 13(6), 624-632.
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2

Western Blot Antibody Specifications

Check if the same lab product or an alternative is used in the 5 most similar protocols
The anti-LMPTP antibody (Ab) was described in 23 . The anti-GAPDH (clone D16H11XP; catalog #5174), anti-pAKT-T308 (catalog #9275), anti-pAKT-S473 (catalog #9271) and anti-pERK-T202/Y204 (catalog #9101) Abs were purchased from Cell Signaling Technology. The anti-IRβ Ab was purchased from Santa Cruz Biotechnology (clone C-19; catalog #sc-711) and the anti-pIR/pIGFR-Y1162/Y1163 Ab was purchased from Life Technologies (catalog #44-804G). Primary antibodies were used at 1:1000 dilution for Western blotting. HRP-conjugated anti-rabbit and anti-mouse IgG Abs were purchased from GE Healthcare (catalog #NA934-1ML and NA931-1ML respectively) and used at 1:3000 dilution. Unless otherwise specified, chemicals and all other reagents were purchased from Sigma-Aldrich.
Stanford S.M., Aleshin A.E., Zhang V., Ardecky R.J., Hedrick M.P., Zou J., Ganji S.R., Bliss M.R., Yamamoto F., Bobkov A.A., Kiselar J., Liu Y., Cadwell G.W., Khare S., Yu J., Barquilla A., Chung T.D., Mustelin T., Schenk S., Bankston L.A., Liddington R.C., Pinkerton A.B, & Bottini N. (2017). Diabetes reversal by inhibition of the low molecular weight tyrosine phosphatase. Nature chemical biology, 13(6), 624-632.
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