The human hepatoblastoma cell line HepG2 cells were purchased from Summit Pharmaceuticals International (Tokyo, Japan). HepG2 cells were cultured in DMEM (Thermo Fisher Scientific) supplemented with 10% FBS (Thermo Fisher Scientific) and 100-µg/ml gentamicin (Thermo Fisher Scientific). HepG2 cells were seeded onto 12-well plates (Becton Dickinson Labware, NJ). To induce steatosis, HepG2 cells were exposed to 0.5-mM free fatty acid (FFA) mixture (oleic acid/palmitic acid, 2:1) (Sigma-Aldrich, St. Louis, MO) for 24 h. HepG2 cells were divided into the following two groups: 1) FFA-treated group; and 2) FFA and BMSC co-culture group. Then, 5 × 104 BMSCs were seeded onto the 0.4-µm-pore size Cell Culture Insert (Becton Dickinson Labware) and placed into the 12-well plate with the HepG2 cells that were initially seeded. In the transwell system, only secretome from BMSC cultured in the upper compartment could pass through the membrane to contact with HepG2 cells cultured in the lower compartment. After another 48 h, the cells were collected for Oil-red O staining (Sigma-Aldrich) and thereafter, incubated for 5 min in 100% isopropanol (Wako). The Oil-red O staining solution was then extracted from each well, and absorbance at a wavelength of 492 nm was measured using the Infinite M 200Pro (Tecan, Kanagawa, Japan).
Hepg2
Manufactured by BD
Sourced in United States
HepG2 is a cell line derived from human hepatocellular carcinoma. It is commonly used in in vitro studies related to liver physiology and pathology.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Facscalibur, by BD (2 mentions)
Cell culture insert, by BD (1 mentions)
Hepg2 cell, by Thermo Fisher Scientific (1 mentions)
12 well plate, by BD (1 mentions)
Gentamicin, by Thermo Fisher Scientific (1 mentions)
Oil red o staining, by Merck Group (1 mentions)
Hepg2 cells, by Merck Group (1 mentions)
Hepg2, by Merck Group (1 mentions)
Infinite m200 pro, by Tecan (1 mentions)
Rat tail collagen 1, by BD (1 mentions)
Female swiss mice, by Janvier Labs (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
Hb 8065, by American Type Culture Collection (1 mentions)
Hepa1 6 cells, by American Type Culture Collection (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Hepg2, by Thermo Fisher Scientific (1 mentions)
Hepg2, by Miltenyi Biotec (1 mentions)
4 protocols using hepg2
1
Coculture of HepG2 Cells with BMSCs for Steatosis Induction
2
Rodent Malaria Parasite Culture
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We used GFP-expressing P. berghei (PbGFP, ANKA strain) and P. yoelii (PyGFP, 17XNL strain) parasite lines, obtained after integration of a GFP expression cassette at the dispensable p230p locus [19 ]. PbGFP and PyGFP blood stage parasites were propagated in female Swiss mice (6–8 weeks old, from Janvier Labs). Anopheles stephensi mosquitoes were fed on PyGFP or PbGFP-infected mice using standard methods [20 ], and kept at 24°C and 21°C, respectively. PyGFP and PbGFP sporozoites were collected from the salivary glands of infected mosquitoes 14–18 or 21–28 days post-feeding, respectively. HepG2 (ATCC HB-8065), HepG2/CD81 [14 (link)] and Hepa1-6 cells (ATCC CRL-1830) were cultured at 37°C under 5% CO2 in DMEM supplemented with 10% fetal calf serum, L-glutamine and antibiotics (Life Technologies), as described [16 (link)]. HepG2 and HepG2/CD81 were cultured in culture dishes coated with rat tail collagen I (Becton Dickinson, Le Pont de Claix, France).
3
Isolation of CD133+ HCC Cells
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The HCC cell lines HepG2 and Huh7 were purchased from the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (Shanghai, China) and maintained in RPMI-1640 (Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Gibco) at 37°C in a humidified 5% CO2 incubator. For separation between CD133+ HCC cells and CD133- HCC cells, HepG2 and Huh7 cells were incubated with fluorescein isothiocyanate (FITC) conjugated CD133 antibody (Miltenyi Biotec, Germany) for 20 min at room temperature. Subsequently, CD133+ and CD133- HepG2 and Huh7 cells were sorted by FACS vantage (FACSCALIBUR, BD Biosciences, USA).
4
Generating HepG2 Cell Lines for Study
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The human hepatoma cell line HepG2, human embryonic kidney cell line 293T and human normal liver cell line L02 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). The human hepatoma cell line LM3 was kindly provided by Chunping Cui (Beijing Institute of Lifeomics, Beijing, China). The HepG2-4D14 cell lines, derived from HepG2 cells by integrating full-length HBV genome in the cellular genome, were kindly provided by Dongping Xu at the 302 Hospital of People’s Liberation Army of China. The cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Invitrogen, Carlsbad, CA, USA) with 10% (v/v) fetal bovine serum (FBS, Pan Biotech, Adenbach, Germany) and maintained in a 37 °C humidified incubator with 5% CO2. The 293T cells were transfected with pll3.7-LINC01010 and the lentiviral packaging plasmids for 48 h. Then the virus particles in the supernatant were harvested to infect HepG2 cells for 12 h. Stable LINC01010-overexpressing cells were sorted by fluorescence-activated cell sorting (FACS) using the FACS Calibur flow cytometer instrument (BD Biosciences, San Jose, CA, USA).
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