Enhanced chemiluminescence ecl assay kit

Madin-Darby canine kidney cells (MDCK) were obtained from American Type Culture Collection (ATCC CCL-34TM, Rockville, USA). H1N1 influenza virus was isolated from clinical samples of Guangzhou Women and Children’s Medical Center, Guangzhou Medical University (Guangzhou, China). Fetal bovine serum (FBS), pancreatic enzymes, and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Gibco (California, USA). P-ATM, P-ATR, PARP, Cleaved PARP, Caspase-3, Cleaved Caspase-3, and β-actin antibodies were purchased from Cell Signaling Technology (Boston, USA). Cell counting kit-8 (CCK-8 kit), reverse transcription-polymerase chain reaction kit (RT-PCR kit), JC-1 Mitochondrial Membrane Potential Assay Kit, Cell Cycle Assay Kit, Annexin-V-Propidium iodide (PI) Co-staining Kit, and Enhanced Chemiluminescence (ECL) Assay Kit were obtained from Beyotime Biotechnology (Shanghai, China). Tunel, 4′, 6-diamidino-2-phenylindole (DAPI), and 2′, 7′-dichlorofluorescein diacetate (DCF-DA) were purchased from Sigma-Aldrich (St. Louis, USA). The detection of the cells and the cytokine were derived from the BD FACSCanto II flow cytometer (Franklin Lakes, USA). Ebselen was provided by Tianfeng Chen, College of Chemistry and Materials Science, Jinan University (Guangzhou, China).